A5029828751- Optical Imaging and Spectroscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Vestibular and auditory disorders
- Medical Imaging Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bat Biology and Ecology Studies
- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- Oral microbiology and periodontitis research
- Photoreceptor and optogenetics research
- Anesthesia and Neurotoxicity Research
- Lipid metabolism and biosynthesis
- Congenital Heart Disease Studies
- Dental Implant Techniques and Outcomes
- Collagen: Extraction and Characterization
- Optical measurement and interference techniques
- Congenital heart defects research
- Metabolomics and Mass Spectrometry Studies
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Angiogenesis and VEGF in Cancer
- Endodontics and Root Canal Treatments
- 3D Printing in Biomedical Research
Laser Zentrum Hannover
2016-2023
Hearing4all
2019
Bielefeld University
2013-2016
Abstract Organoid models of early tissue development have been produced for the intestine, brain, kidney and other organs, but similar approaches heart lacking. Here we generate complex, highly structured, three-dimensional heart-forming organoids (HFOs) by embedding human pluripotent stem cell aggregates in Matrigel followed directed cardiac differentiation via biphasic WNT pathway modulation with small molecules. HFOs are composed a myocardial layer lined endocardial-like cells surrounded...
Cellular lipid droplets are the least studied and understood cellular organelles in eukaryotic prokaryotic cells. Despite a significant body of research studying physiology it has not yet been possible to fully determine composition individual droplets. In this paper we use Raman spectroscopy on single least-squares fitting pure fatty acid spectra cells after treatment with different ratios oleic palmitic acid. We validate results spectroscopy-based droplet analysis obtained by gas...
Tissue engineering by stem cell differentiation is a novel treatment option for bone regeneration. Most approaches the detection of osteogenic are invasive or destructive and not compatible with live analysis. Here, non-destructive label-free Raman spectroscopy, coherent anti-Stokes scattering (CARS) second harmonic generation (SHG) microscopy were used to detect image human neural crest-derived inferior turbinate cells (ITSCs). Combined CARS SHG was able markers osteogenesis within 14 days...
The mammalian cochlea is a complex macroscopic structure due to its helical shape and the microscopic arrangements of individual layers cells. To improve outcomes hearing restoration in deaf patients, it important understand anatomic composition ex vivo. Hitherto, only one histological technique based on confocal laser scanning microscopy optical clearing has been developed for toto imaging murine cochlea. However, with growing size specimen, e.g., human cochlea, this reaches limitations....
The present study focuses on the application of scanning laser optical tomography (SLOT) for visualization anatomical structures inside human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique which allows tomographic imaging internal structure transparent specimens. Thus, in field otology this best convenient an vivo inner ear anatomy. For purpose, preparation before comprises decalcification, dehydration as well clearing samples toto. Here, we demonstrate results...
Volumetric imaging of connective tissue provides insights into the structure biological tissue. Second harmonic generation (SHG) microscopy has become a standard method to image collagen rich like skin or cornea. Due non-centrosymmetric architecture, no additional label is needed and can be visualized noninvasively. Thus, SHG enables investigation associated diseases, providing high resolution images field view several hundreds μm. However, in toto visualization larger samples limited...
The triple-negative breast cancer is an aggressive subtype that has a high rate of relapse and poor five-year survival rate. Tumor Microenviroment influences the behaviors such as proliferation, migration formation metastasis. We present complementary imaging subsequent analysis using Lightsheet Microscopy, Scanning Laser Optical Tomography TPEF. samples were imaged in different size resolution scales three-dimensional information obtained SHG-signal collagen fluorescence labeled key...
Abstract Developmental neurotoxic compounds impair the developing human nervous system at lower doses than those affecting adults. Standardized test methods for assessing developmental neurotoxicity (DNT) require use of high numbers laboratory animals. Here, we a novel assay that is based on development an intact insect embryo in serum-free culture. Neural pathways leg embryonic locusts are established by pair afferent pioneer neurons, extending axons along well-defined pathway to central...
The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization anatomical structures inside human cochlea <i>ex vivo</i>. SLOT is a laser-based highly efficient microscopy technique, which allows tomographic imaging internal structure transparent large-scale specimens (up to 1 cm<sup>3</sup>). Thus, in field otology this technique best convenient an vivo</i> inner ear anatomy. For purpose, preparation before comprises mechanically...
Scanning laser optical tomography (SLOT) is a 3D imaging technique, based on the principle of computed to visualize samples up magnitude several centimeters. Intrinsic contrast mechanisms as absorption, scattering and autofluorescence provide information about architecture composition sample. Another valuable intrinsic mechanism second harmonic generation (SHG), which generated in noncentrosymmetric materials commonly used image collagen biological samples. The angular dependence SHG signal,...
It is estimated that two million new dental implants are inserted worldwide each year. Innovative implant materials developed in order to minimize the risk of peri-implant inflammations. The broad range material testing conducted using standard 2D, terminal, and invasive methods. methods have been applied not sufficient monitor whole surface temporal progress. Therefore, we built a 3D model cylindrical colonized by human gingival fibroblasts. In cell response over time, non-toxic LIVE/DEAD...
The triple-negative breast cancer (tnbc) is an aggressive subtype linked to a poor outcome of established therapy. Increasing evidence points the role tumor's extracellular matrix (ECM) as determinant its aggressiveness well effectiveness chemical therapeutics. Three-dimensional imaging techniques can be used unravel ECM architecture. Label-free contrast mechanisms such second harmonic generation (SHG) avoid falsification and artifacts introduced by labeling process. Here, we present...
The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization anatomical structures inside human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique, which allows tomographic imaging internal structure transparent large-scale specimens (up to 1 cm3). Thus, in field otology this technique best convenient an vivo inner ear anatomy. For purpose, preparation before comprises mechanically assisted...
Triple-negative breast cancer is an aggressive subtype of that has a poor five-year survival rate. The tumor’s extracellular matrix major compartment its microenvironment and influences the proliferation, migration formation metastases. study such dependencies requires methods to analyze tumor in native form. In this work, limits SHG-microscopy, namely limited penetration depth, sample size specificity, are addressed by correlative three-dimensional imaging. We present combination scanning...
The extracellular matrix plays a crucial role in tumor development and efficacy of chemotherapy. We present combined imaging approach to investigate quantify the collagen architecture on mm μm scale.