A5091015065- Oral microbiology and periodontitis research
- Dental Implant Techniques and Outcomes
- Laser-Ablation Synthesis of Nanoparticles
- Bone Tissue Engineering Materials
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- 3D Printing in Biomedical Research
- Dental materials and restorations
- Endodontics and Root Canal Treatments
- Health Systems, Economic Evaluations, Quality of Life
- Nanoparticles: synthesis and applications
- Herpesvirus Infections and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hemoglobinopathies and Related Disorders
- Blood disorders and treatments
- Reconstructive Surgery and Microvascular Techniques
- Cytomegalovirus and herpesvirus research
- Pharmaceutical Economics and Policy
- Orthopedic Infections and Treatments
- Graphene and Nanomaterials Applications
- Laser Applications in Dentistry and Medicine
- Iron Metabolism and Disorders
- Pharmaceutical studies and practices
Medizinische Hochschule Hannover
2015-2024
Aristotle University of Thessaloniki
2010-2023
Abstract Staphylococcus aureus biofilm-associated infections are a common complication in modern medicine. Due to inherent resilience of biofilms antibiotics and the rising number antibiotic-resistant bacterial strains, new treatment options required. For this purpose, ultrapure, spherical silver–gold-alloy nanoparticles with homogenous elemental distribution were synthesized by laser ablation liquids analyzed for their antibacterial activity on different stages S. biofilm formation as well...
The impact of oral commensal and pathogenic bacteria on peri-implant mucosa is not well understood, despite the high prevalence infections. Hence, we investigated responses to Streptococcus oralis or Aggregatibacter actinomycetemcomitans biofilms using a novel in vitro mucosa-biofilm model. Our 3D model combined three components, organotypic mucosa, implant material, biofilm, with structural assembly close native situation. S. induced protective stress response through upregulation heat...
Abstract The bactericidal effects of silver nanoparticles (Ag NPs) against infectious strains multiresistant bacteria is a well‐studied phenomenon, highly relevant for many researchers and clinicians battling bacterial infections. However, little known about the uptake Ag NPs into bacteria, related mechanisms, how they are connected to antimicrobial activity. Even less information available on AgAu alloy uptake. In this work, interactions between colloidal silver–gold (AgAu Staphylococcus...
The host-microbe relationship is pivotal for oral health as well peri-implant diseases. Peri-implant mucosa and commensal biofilm play important roles in the maintenance of homeostasis, but little known about how they interact. We have therefore investigated early interaction between multispecies (Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar, Porphyromonas gingivalis) organotypic using our three-dimensional model. After 24 hr, biofilms induced weak inflammatory reaction...
Human gingival epithelial cells (HGEps) and fibroblasts (HGFs) are the main cell types in peri-implant soft tissue. HGEps constantly exposed to bacteria, but HGFs protected by connective tissue as long mucosa-implant seal is intact. Streptococcus oralis one of commensal highly abundant at healthy implant sites, might modulate cells-as has been described for other streptococci. We have therefore investigated effects S. biofilm on HGFs. or were grown separately titanium disks responded...
Human cytomegalovirus (HCMV) harmfully impacts survival after peripheral blood hematopoietic stem cell transplantation (PB-HSCT). Delayed immune reconstitution cord (CB)-HSCT leads to even higher HCMV-related morbidity and mortality. Towards a feasible dendritic therapy accelerate de novo immunity against HCMV, we validated tricistronic integrase-defective lentiviral vector (coexpressing GM-CSF, IFN-α, HCMV pp65 antigen) capable directly induce self-differentiation of PB CB monocytes into...
Cytokine profiles are often perturbed after infections of medical implants. With a non-invasive in vivo imaging system, we report mouse model that interferon expression infection subcutaneous implants with Streptococcus oralis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola (alone or as combination) was species-specific, persisted longer the presence implants, notably decreased upon dual species infections. This type I disappeared within two weeks;...
Oral innate immunity is led by neutrophils. It still unclear how their main antimicrobial mechanisms against different biofilms may contribute to balance or dysregulation in the oral cavity. We investigated capacity of commensal (Streptococcus oralis) and pathogenic (Porphyromonas gingivalis Aggregatibacter actinomycetemcomitans) monospecies induce inhibit selected S. oralis induced neutrophil extracellular traps (NETs) formation, reactive oxygen species (ROS) production, matrix...
Protein replacement therapy (PRT) has been applied to treat severe monogenetic/metabolic disorders characterized by a protein deficiency. In where an intracellular is missing, PRT not easily feasible due the inability of proteins cross cell membrane. Instead, gene applied, although still with limited success. β-Thalassemias are congenital hemoglobinopathies, deficiency or reduced production adult β-globin chain. The resulting imbalance α-/β-globin chains hemoglobin (α2β2) leads precipitation...
Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration an in-depth understanding interaction between native tissue surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary lines fibroblasts osteoblasts included quantification area, aspect ratio, focal adhesions. Furthermore, we examined...
Abstract Staphylococcus aureus biofilm-associated infections are a common complication in modern medicine. Due to inherent resilience of biofilms antibiotics and the rising number antibiotic-resistant bacterial strains, new treatment options required. For this purpose, ultrapure, spherical silver-gold-alloy nanoparticles with homogenous elemental distribution were synthesized by laser ablation liquids analyzed for their antibacterial activity on different stages S. biofilm formation as well...
Additive manufacturing technologies such as laser powder bed fusion (L-PBF) offer the versatility in material design required to produce parts suitable for aeronautic and medical applications. However, library of materials processable by L-PBF is limited, reducing possibility with custom functionalities. To address this limitation, incorporation nanoparticles base has been proven widen L-PBF-processable (i.e., TPU) provide additional functionalities already employed polyamide 12 (PA12). In...
It is estimated that two million new dental implants are inserted worldwide each year. Innovative implant materials developed in order to minimize the risk of peri-implant inflammations. The broad range material testing conducted using standard 2D, terminal, and invasive methods. methods have been applied not sufficient monitor whole surface temporal progress. Therefore, we built a 3D model cylindrical colonized by human gingival fibroblasts. In cell response over time, non-toxic LIVE/DEAD...
A symbiotic microbial community of commensals exists during oral health.Pathogenic bacteria are able to induce dysregulation hostmicrobiota homeostasis and a dysbiotic biofilm formation can cause peri-implantitis, chronic infection.Little is known about the role commensal pathogenic in regulation balance between host tolerance inflammation.Previous studies have indicated that related disease prevention, but details modulatory effects still missing.Moreover, knowledge influence implant...