A5068303871- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Optical Coherence Tomography Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Bee Products Chemical Analysis
- Lipid metabolism and biosynthesis
- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Identification and Quantification in Food
- Molecular Biology Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Ocular and Laser Science Research
- Metabolomics and Mass Spectrometry Studies
- Plasmonic and Surface Plasmon Research
- Bacterial Identification and Susceptibility Testing
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Laser-induced spectroscopy and plasma
- Water Quality Monitoring and Analysis
- T-cell and B-cell Immunology
- Smart Agriculture and AI
- Genetics, Aging, and Longevity in Model Organisms
- Algal biology and biofuel production
- Electrowetting and Microfluidic Technologies
- Near-Field Optical Microscopy
Leibniz Institute of Photonic Technology
2015-2024
University of Duisburg-Essen
2024
Carl Zeiss (Germany)
2019-2024
Ernst Abbe University of Applied Sciences Jena
2019-2023
Frankfurt University of Applied Sciences
2021
Medical University of Vienna
2021
Gentofte Hospital
2021
University of Vienna
2021
Institute of Physical Chemistry
2019
Friedrich Schiller University Jena
2016-2019
We present a high-throughput screening Raman spectroscopy (HTS-RS) platform for rapid and label-free macromolecular fingerprinting of tens thousands eukaryotic cells. The newly proposed HTS-RS combines automated imaging microscopy with to enable cells can be applied large number biomedical clinical applications. potential the new approach is illustrated by two (1) HTS-RS-based differential white blood cell count. A classification model was trained using spectra 52 218 lymphocytes, 48 220...
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTRaman Spectroscopy and Imaging in BioanalyticsDana Cialla-MayDana Cialla-MayLeibniz-Institute of Photonic Technology, Member the Leibniz Research Alliance − Health Technologies, Albert-Einstein-Str. 9, 07745 Jena, GermanyInstitute Physical Chemistry Abbe Center Photonics, Friedrich Schiller University, Helmholtzweg 4, 07743 GermanyInfectoGnostics Campus Applied Research, Philosophenweg 7, GermanyMore by Dana Cialla-MayView Biography, Christoph...
The steady progress in medical diagnosis and treatment of diseases largely hinges on the development improvement modern imaging modalities. Raman spectroscopy has attracted increasing attention for clinical applications as it is label-free, non-invasive, delivers molecular fingerprinting information a sample. In combination with fiber optic probes, also allows easy access to different body parts patient. However, image acquisition probes currently not possible. Here, we introduce probe-based...
Raman spectroscopy is a powerful tool for label-free, single cell characterization. In many reported studies, spectrum acquired from fraction of the volume and used as representative signature whole to identify discriminate between populations. It has remained an open question whether this most suitable approach since spectra may not truly represent critical biochemical information could therefore be lost. To address question, we developed line-scan microscope acquire images lymphocytes...
Cellular lipid droplets are the least studied and understood cellular organelles in eukaryotic prokaryotic cells. Despite a significant body of research studying physiology it has not yet been possible to fully determine composition individual droplets. In this paper we use Raman spectroscopy on single least-squares fitting pure fatty acid spectra cells after treatment with different ratios oleic palmitic acid. We validate results spectroscopy-based droplet analysis obtained by gas...
Raman spectroscopy provides label-free biochemical information from tissue samples without complicated sample preparation. The clinical capability of has been demonstrated in a wide range vitro and vivo applications. However, challenge for applications is the simultaneous excitation auto-fluorescence majority tissues interest, such as liver, bladder, brain, others. bands are then superimposed on fluorescence background, which can be several orders magnitude larger than signal. To eliminate...
Neutrophils are important cells of the innate immune system and major leukocyte subpopulation in blood. They responsible for recognizing neutralizing invading pathogens, such as bacteria or fungi. For this, neutrophils well equipped with pathogen receptors, cytokines, effector molecules, granules filled reactive oxygen species (ROS)-producing enzymes. Depending on type, different reactions triggered, which result specific activation states neutrophils. Here, we aim to establish a label-free...
Integrated acquisition of Raman spectra gives better results for classification tumor and non-tumor cells.
We present a combination of optical coherence tomography (OCT) and Raman spectroscopy (RS) for improved diagnosis discrimination different stages grades bladder cancer ex vivo by linking the complementary information provided these two techniques. Bladder samples were obtained from biopsies dissected via transurethral resection tumor (TURBT). As OCT provides structural rapidly, it was used as red-flag technology to scan wall suspicious lesions with ability discriminate malignant tissue...
Existing approaches for early-stage bladder tumor diagnosis largely depend on invasive and time-consuming procedures, resulting in hospitalization, bleeding, perforation, infection other health risks the patient. The reduction of current risk factors, while maintaining or even improving diagnostic precision, is an underlying factor clinical instrumentation research. For example, clinic surveillance patients with a history noninvasive tumors real-time can enable immediate laser-based removal...
Non-muscle-invasive bladder cancer affects millions of people worldwide, resulting in significant discomfort to the patient and potential death. Today, cystoscopy is gold standard for assessment, using white light endoscopy detect tumor suspected lesion areas, followed by resection these areas subsequent histopathological evaluation. Not only does pathological examination take days, but due invasive nature, performed biopsy can result harm patient. Nowadays, optical modalities, such as...
Abstract The increased accumulation of intracellular lipid droplets within hepatocytes is a pathologic hallmark liver injury various etiologies, especially non‐alcoholic steatohepatitis (NASH). dynamics, subcellular origin, and chemical composition under pathophysiologic conditions, however, remain poorly understood. We used coherent Raman microscopy spontaneous spectroscopy to monitor analyze the formation in living primary rat exposed triglyceride‐rich lipoprotein (TGRL) lipolysis...
The throughput of spontaneous Raman spectroscopy for cell identification applications is limited to the range one per second because relatively low sensitivity. Surface-enhanced scattering (SERS) a widespread way amplify intensity signals by several orders magnitude and, consequently, improve sensitivity and throughput. SERS protocols using immuno-functionalized nanoparticles turned out be challenging they require complex preparation procedures. Here, new strategy presented classification...
Abstract Shifted excitation Raman difference spectroscopy (SERDS) is a background correction method for spectroscopy. Here, the spectra were directly used as input SERDS-based classification after an optimization procedure to correct photobleaching of autofluorescence. Further processing included principal component analysis compensate reduced signal noise ratio and subsequent by linear discriminant analysis. As case study 6,028 single pollen originating from plants eight different genera...
We present a novel scheme to simultaneously detect coherent anti-Stokes Raman scattering (CARS) microscopy signals in the forward (F) and backward (epi - E) direction with single avalanche photodiode (APD) detector using time-correlated photon counting (TCSPC). By installing mirror at well-defined distance above sample forward-scattered F-CARS signal is reflected back into microscope objective leading spatial overlap of F E-CARS signals. Due traveling an additional time delayed relative...
We demonstrate that doubly-resonant coherent anti-Stokes Raman scattering can provide enhanced and highly specific contrast for molecules containing unique Raman-active small molecular groups. This combination provides otherwise be difficult to discriminate by spectroscopy. Here, human monocytes were incubated with either deuterated oleic acid or 17-octadecynoic (a fatty an end terminal acetylene group). The carbon-deuterium stretching vibration of the acid, as well alkyne alkyne-containing...
Typical commercial Raman micro-spectroscopic systems do not offer much flexibility to the end user, thus limiting potential research applications.We present a design of simple, highly flexible and portable confocal microscope with detailed list parts.The system can perform spectral acquisition in different modes: single-point spectroscopy, hyperspectral point mapping or line mapping.Moreover, be easily converted between inverted upright configurations, which beneficial for specific...