A5013298312- Lipid metabolism and biosynthesis
- Dialysis and Renal Disease Management
- Therapeutic Uses of Natural Elements
- Cardiovascular Function and Risk Factors
- Cardiac electrophysiology and arrhythmias
- Plant Surface Properties and Treatments
- Plant nutrient uptake and metabolism
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Soybean genetics and cultivation
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Retinal Development and Disorders
- Parathyroid Disorders and Treatments
- Nitric Oxide and Endothelin Effects
- Neurological disorders and treatments
- Plant biochemistry and biosynthesis
- Pancreatitis Pathology and Treatment
- Potato Plant Research
- Blood Pressure and Hypertension Studies
- Thyroid Disorders and Treatments
- Retinal Diseases and Treatments
- Metabolism and Genetic Disorders
- Galectins and Cancer Biology
- Neurological and metabolic disorders
- Potassium and Related Disorders
Case Western Reserve University
2019-2023
University School
2019-2023
Seqirus (United States)
2023
University Hospitals of Cleveland
2021-2022
Central Institute of Medicinal and Aromatic Plants
2012
Indian Institute of Science Bangalore
2010-2012
Council of Scientific and Industrial Research
2012
A key step in the triacylglycerol (TAG) biosynthetic pathway is final acylation of diacylglycerol (DAG) by DAG acyltransferase. In silico analysis has revealed that DCR (defective cuticular ridges) (At5g23940) gene a typical HX4D acyltransferase motif at N-terminal end and lipid binding VX2GF middle sequence. To understand biochemical function, was overexpressed Escherichia coli, purified recombinant protein found to acylate specifically an acyl-CoA-dependent manner. Overexpression At5g23940...
<h3>Rationale & Objective</h3> Cardiovascular disease (CVD) is a major cause of mortality among people with diabetic kidney (DKD). The pathophysiology inadequately explained by traditional CVD risk factors. uremic solutes trimethylamine-<i>N</i>-oxide (TMAO) and asymmetric symmetric dimethylarginine (ADMA, SDMA) have been linked to in failure replacement therapy (KFRT), but data are limited populations diabetes less severe disease. <h3>Study Design</h3> Observational cohort. <h3>Settings...
ABSTRACT Background Chronic kidney disease (CKD) is associated with atherosclerotic cardiovascular (ASCVD) risk, especially among those diabetes. Altered metabolism of solutes that accumulate in CKD [asymmetric dimethylarginine (ADMA), symmetric (SDMA) and trimethylamine N-oxide (TMAO)] may reflect pathways linking ASCVD. Methods This case–cohort study included Renal Insufficiency Cohort participants baseline diabetes, estimated glomerular filtration rate &lt;60 mL/min/1.73 m2, without...
The biosynthesis of triacylglycerol (TAG) occurs in the microsomal membranes eukaryotes. Here, we report identification and functional characterization diacylglycerol acyltransferase (DGAT), a member 10 S cytosolic TAG biosynthetic complex (TBC) Rhodotorula glutinis. Both full-length an N-terminally truncated cDNA clone single gene were isolated from R. DGAT activity protein encoded by RgDGAT was confirmed vivo heterologous expression Saccharomyces cerevisiae quadruple mutant (H1246) that is...
Abstract Monoacylglycerol acyltransferase (MGAT) catalyzes the synthesis of diacylglycerol, precursor triacylglycerol biosynthesis and an important signaling molecule. Here, we describe isolation characterization peanut (Arachis hypogaea) MGAT gene. The soluble enzyme utilizes invariant histidine-62 aspartate-67 residues motif for its activity. A sequence analysis revealed presence a hydrolase (GXSXG) motif, assays monoacylglycerol (MAG) lysophosphatidylcholine (LPC) hydrolytic activities,...
Purpose: Clinical investigations associate hypothyroidism with an increased risk for microvascular complications, yet the mechanism by which thyroid hormone regulates development of diabetic retinopathy is not clearly understood. We investigated role iodothyronine deiodinase 2 (DIO2) in pathogenesis retinopathy. Methods: Retinas from streptozotocin-induced and nondiabetic mice were evaluated RNA sequencing, RT-PCR, immunostaining. Media cell lysates mouse retinal endothelial cells astrocytes...
Abstract Chronic kidney disease (CKD) affects more than 20 million people in the US, and it is associated with a significantly increased risk of sudden cardiac death (SCD). Despite significance, mechanistic relationship between SCD CKD not clear there are few effective therapies. Using optical mapping techniques, we tested hypothesis that mouse models progressive diabetic (DKD) exhibit enhanced ventricular arrhythmia incidence underlying substrates. Compared to wild-type mice, both Lepr...
Abstract Background and Aims Circulating cardiac biomarkers implicated in the pathogenesis of heart disease are a non-invasive platform to assess cardiovascular (CVD) burden individuals with chronic kidney (CKD). Galectin 3 is 26 kDa β-galactoside-binding lectin that has graded positive association CKD stages. In animal models, inhibition galectin-3 prevents myocardial fibrosis. These pre-clinical findings have not been replicated randomized controlled studies humans. Metabolic acidosis...
Journal of the American Society Nephrology 32(10S):p 47, October 2021. | DOI: 10.1681/ASN.20213210S147b