A5101404481- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Reproductive tract infections research
- Antibiotic Use and Resistance
- Pneumonia and Respiratory Infections
- Metabolism and Genetic Disorders
- Streptococcal Infections and Treatments
- Bacterial Infections and Vaccines
- Mycobacterium research and diagnosis
- Diphtheria, Corynebacterium, and Tetanus
- Urinary Tract Infections Management
- Infective Endocarditis Diagnosis and Management
- Metabolomics and Mass Spectrometry Studies
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Infections and bacterial resistance
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
University College London
2014-2024
Institute of Structural and Molecular Biology
2014-2024
Queen Mary University of London
2013-2023
MCPHS University
2021
University of London
1987-2020
University of Greenwich
2020
Canterbury Health Laboratories
2016
Keck Graduate Institute
2012
Weatherford College
2010
Institute of Geography of the Slovak Academy of Sciences
2007-2009
A nomenclature for the P450 gene superfamily is proposed based on evolution. Recommendations include Roman numerals distinct families, capital letters subfamilies, and Arabic individual genes. An updating of this list, which presently includes 65 entries, will be required every 1–2 years. Assignment orthologous genes uncertain in some cases—between widely diverged species especially P450II family due to large number As more known, it might become necessary change assignments that are our...
Seventeen per cent of the staff an intensive care ward were found to have Klebsiella spp contaminating their hands, and these strains could be related serotypes infecting or colonising patients in on same day. We identified some simple procedures that resulted contamination nurses' hands with 100-1000 klebsiellae hand. Klebsiellae survived artifically inoculated for up 150 minutes. Handwashing chlorhexidine hand cleanser reliably gave 98-100% reduction counts, introduction routine...
Twenty-six nurses were repeatedly screened for carriage of epidemic methicillin-resistant Staphylococcus aureus (EMRSA) immediately before and after duty periods in which they solely attended six patients widely colonized with two EMRSA strains distinguishable by plasmid analysis. was detected 13 nurses. Three patterns emerged: transient 12 nurses, when the isolated from noses or fingers but gone their next day's duty; short-term nasal carriage, seen on occasion 4 these consecutive screens;...
The cloning and expression of the full-length tissue inhibitor metalloproteinase 2 (TIMP-2), delta 187-194TIMP-2, 128-194TIMP-2 purification these inhibitors a cleaved version TIMP-2 lacking nine C-terminal amino acids (delta 186-194TIMP-2) are described. mechanism inhibition gelatinase A by TIMPs was investigated comparing kinetics association TIMP-1, TIMP-2, deletions, mutants both which consisted N-terminal domain only. inhibited rapidly with constants 3.2 x 10(6) M-1 s-1 for TIMP-1 2.1...
Three hundred and twenty five episodes of pneumococcal bacteraemia occurred at St Thomas9s Hospital during 1970-84, accounting for 13.3% all bacteraemia. Twice as many cases in male female patients, common predisposing factors included chronic chest disease, alcoholism, haematological malignancies, cirrhosis, sickle cell anaemia. Mortality was 28.6% overall but only 11.8% among patients who received antibiotic treatment least 24 hours. Most (261) had pneumonia, 26 meningitis, eight were...
The API ZYM system of detection enzymes has been applied to 81 bacteria belonging several species. It was found be easy use and produced results that may useful in the identification a variety bacteria.
The 4-quinolones ciprofloxacin, difloxacin, enoxacin, norfloxacin, ofloxacin, and nalidixic acid were found to induce the SOS response in qualitative quantitative tests on Escherichia coli K12 containing sfiA::lacZ gene fusion. Maximum induction of SOS-response was observed with quinolone concentrations that produced most killing. There also a modest increase rate mutation lactose galactose operons GalE- background, provided there functioning system.
Journal Article Epidemic methicillin-resistant Staphylococcus aureus Get access B. D. Cookson, Cookson Department of Microbiology, UMDS, St Thomas' HospitalLondon SE1 7EH, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar I. Phillips Antimicrobial Chemotherapy, Volume 21, Issue suppl_C, 1988, Pages 57–65, https://doi.org/10.1093/jac/21.suppl_C.57 Published: 01 September 1988
Objectives To date, six flavin-containing monooxygenase (FMO) genes have been identified in humans, FMOs 1, 2, 3, 4 and 6, which are located within a cluster on chromosome FMO5, is outside the cluster. The objectives were to review update current knowledge of structure expression profiles these their mouse counterparts determine, via bioinformatics approach, whether other FMO present human genomes. Results conclusions We identified, for first time, Fmo6 gene. In addition, we describe novel...
We have previously described the isolation and, sequencing of cDNA clones encoding thin‐containing monooxygenases (FMOs) 1 and 4 man [Dolphin, C., Shephard, E. A., Povey. S., Palmer, C. N. Ziegler, D. M., Ayesh, R., Smith, R. L. & Phillips, I. (1991) J. Biol. Chem. 266 , 12379–12385; Dolphin, Povey, (1992) Biochem. 287 261–267]. present here a for FMO3 man. The sequence this atnino acid deduced from it differ substantially those reported member FMO family In addition, we investigated, by...
Among Escherichia coli organisms isolated at St. Thomas's Hospital during the years 1990 to 1994, frequency of resistance amoxicillin-clavulanic acid (tested by disk diffusion in a ratio 2:1) remained constant about 5% patient isolates (10 15% 41 45% that were amoxicillin resistant). Mechanisms increased determined for 72 consecutively collected such acid-resistant isolates. MICs combination 16-8 micrograms/ml 51 (71%) these and > or = 32-16 remainder. The predominant mechanism was...
Escherichia coli accounted for 861 (23.9%) of 3,605 episodes bacteremia in an 18-year prospective survey at St. Thomas' Hospital, a proportion that changed little during the survey. The most common focus infection leading to nosocomial and community-acquired due E. was urinary tract. Twenty-six percent adult female patients with resulting from tract were diabetic. O antigen serotypes identified often O6, O2, O1, O4, O15, O75; multiply resistant O15 serotype implicated community outbreak...
Flavin-containing monooxygenases (FMOs) are NADPH-dependent flavoenzymes that catalyze the oxidation of heteroatom centers in numerous drugs and xenobiotics. FMO2, or "pulmonary" FMO, one five forms enzyme identified mammals, is expressed predominantly lung differs from other FMOs it can <i>N</i>-oxidation certain primary alkylamines. We describe here isolation characterization cDNAs for human FMO2. Analysis sequence a section corresponding gene revealed major <i>FMO2</i> allele humans...