A5042875909- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Muscle Physiology and Disorders
- Alzheimer's disease research and treatments
- Tryptophan and brain disorders
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Genetic Neurodegenerative Diseases
- Aquaculture disease management and microbiota
- Viral Infections and Immunology Research
- Critical Race Theory in Education
- Dementia and Cognitive Impairment Research
- Circular RNAs in diseases
- Bioinformatics and Genomic Networks
- Racial and Ethnic Identity Research
- Ubiquitin and proteasome pathways
- Climate Change Communication and Perception
- Biomedical Text Mining and Ontologies
- Protein Structure and Dynamics
- Developmental Biology and Gene Regulation
- Microbial Metabolic Engineering and Bioproduction
- Contact Dermatitis and Allergies
- Occupational exposure and asthma
University of California, San Diego
2021-2025
National Institutes of Health
2019-2020
National Center for Advancing Translational Sciences
2019-2020
The Ohio State University
2018
RNA-binding proteins (RBPs) modulate alternative splicing outcomes to determine isoform expression and cellular survival. To identify RBPs that directly drive exon inclusion, we developed tethered function luciferase-based reporters provide rapid, scalable robust readouts of inclusion changes used these evaluate 718 human RBPs. We performed enhanced cross-linking immunoprecipitation, RNA sequencing affinity purification-mass spectrometry investigate a subset candidates with no prior...
Abstract Huntington’s disease (HD) is a fatal, dominantly inherited neurodegenerative disorder caused by CAG trinucleotide expansion in exon 1 of the huntingtin ( HTT ) gene. Since reduction pathogenic mutant messenger RNA therapeutic, we developed allele-sensitive EX RNA-targeting CRISPR–Cas13d system (Cas13d–CAG that eliminates toxic fibroblasts derived from patients with HD and induced pluripotent stem cell-derived neurons. We show intrastriatal delivery Cas13d–CAG via an adeno-associated...
RNA-binding proteins (RBPs) control RNA metabolism to orchestrate gene expression and, when dysfunctional, underlie human diseases. Proteome-wide discovery efforts predict thousands of RBP candidates, many which lack canonical domains (RBDs). Here, we present a hybrid ensemble classifier (HydRA), leverages information from both intermolecular protein interactions and internal sequence patterns capacity with unparalleled specificity sensitivity using support vector machines (SVMs),...
Skeletal muscle atrophy is a morbidity and mortality risk factor that happens with disuse, chronic disease, aging. The tissue remodeling during recovery from or injury involves changes in different cell types such as fibers, satellite immune cells. Here, we show the previously uncharacterized gene protein Zfp697 damage-induced regulator of remodeling. Zfp697/ZNF697 expression transiently elevated mice humans. Sustained mouse leads to signature chemokine secretion, recruitment, extracellular...
Chemical-peptide conjugation is the molecular initiating event in skin sensitization. The OECD test guideline uses a high-performance liquid chromatography/ultraviolet (HPLC/UV) detection method to quantify chemical-peptide direct peptide reactivity assay (DPRA), which measures depletion of two synthetic peptides containing lysine or cysteine residues. To improve throughput, sensitivity and accuracy, an automated 384-well plate-based RapidFire solid-phase extraction (SPE) system coupled with...
Quality control monitoring of cell lines utilized in biomedical research is utmost importance and critical for the reproducibility data. Two key pitfalls tissue culture are 1) line authenticity 2)
Muscular atrophy is a mortality risk factor that happens with disuse, chronic disease, and aging. Recovery from requires changes in several cell types including muscle fibers, satellite immune cells. Here we show Zfp697/ZNF697 damage-induced regulator of regeneration, during which its expression transiently elevated. Conversely, sustained Zfp697 mouse leads to gene signature chemokine secretion, recruitment, extracellular matrix remodeling. Myofiber-specific ablation hinders the inflammatory...
Abstract Huntington’s disease (HD) is a fatal, dominantly inherited neurodegenerative disorder caused by CAG trinucleotide expansion in exon 1 of the huntingtin ( HTT ) gene. Although pathogenesis HD remains complex, CAG-expanded (CAG EX mRNA and protein ultimately causes through toxic gain-of-function mechanism. As reduction pathogenic mutant beneficial as treatment, we developed RNA-eliminating CRISPR-Cas13d system (Cas13d/CAG that binds eliminates RNA patient iPSC-derived striatal...
RNA-binding proteins (RBPs) control RNA metabolism to orchestrate gene expression, and dysfunctional RBPs underlie many human diseases. Proteome-wide discovery efforts predict thousands of novel RBPs, which lack canonical domains. Here, we present a hybrid ensemble RBP classifier (HydRA) that leverages information from both intermolecular protein interactions internal sequence patterns capacity with unparalleled specificity sensitivity using support vector machine, convolutional neural...
Alzheimer's disease (AD) redefined: For the past century, AD has been defined as a of progressive cognitive decline paired with burden amyloid-β (Aβ) plaques and pathologic tau tangles in hippocampus forebrain. However, recent Framework paper jointly sponsored by National Institute on Aging Association (Jack et al., 2018) proposes new classification guidelines for AD, which, if adopted, will have profound consequences future management AD. The redefine terms brain's burdens Aβ to lesser...
Abstract Quality control monitoring of cell lines utilized in biomedical research is critical importance, and for reproducibility data. Two key pitfalls tissue culture are 1) line authenticity, 2) mycoplasma contamination. As a collaborative institute, the National Center Advancing Translational Sciences receives from range commercial academic sources, that adapted high-throughput screening. Here, we describe implementation routine NCATS-wide testing short-tandem repeat (STR) lines. Initial...
Abstract RNA-binding proteins (RBPs) control RNA metabolism to orchestrate gene expression, and dysfunctional RBPs underlie many human diseases. Proteome-wide discovery efforts predict thousands of novel RBPs, which lack canonical domains. Here, we present a hybrid ensemble RBP classifier (HydRA) that leverages information from both intermolecular protein interactions internal sequence patterns capacity with unparalleled specificity sensitivity using support vector machine, convolutional...