A5033652132- RNA Research and Splicing
- melanin and skin pigmentation
- Genomics and Chromatin Dynamics
- Chronic Kidney Disease and Diabetes
- Connective Tissue Growth Factor Research
- Renal Diseases and Glomerulopathies
- Developmental Biology and Gene Regulation
- Diabetes Treatment and Management
- RNA modifications and cancer
- Skin Protection and Aging
- Cannabis and Cannabinoid Research
- Psychosocial Factors Impacting Youth
- Biochemical Analysis and Sensing Techniques
- Extracellular vesicles in disease
- Pluripotent Stem Cells Research
- Metabolism, Diabetes, and Cancer
- 3D Printing in Biomedical Research
- Renal Transplantation Outcomes and Treatments
- COVID-19 and healthcare impacts
- Health and Wellbeing Research
- Pharmacy and Medical Practices
- Cytomegalovirus and herpesvirus research
- Molecular Biology Techniques and Applications
- Job Satisfaction and Organizational Behavior
- Phytochemicals and Antioxidant Activities
Seoul National University
2023-2024
Kyung Hee University
2023-2024
Seoul National University Bundang Hospital
2022-2024
Montreal Clinical Research Institute
2020-2024
Arizona State University
2021-2024
Dankook University Hospital
2023
Harvard University
2007-2018
Massachusetts General Hospital
2007-2018
Jeju National University
2015-2017
Dong-Eui University
2013-2016
The inefficient clearance of dying cells can lead to abnormal immune responses, such as unresolved inflammation and autoimmune conditions. We show that tumor suppressor p53 controls signaling-mediated phagocytosis apoptotic through its target, Death Domain1α (DD1α), which suggests promotes both the proapoptotic pathway postapoptotic events. DD1α appears function an engulfment ligand or receptor engages in homophilic intermolecular interaction at intercellular junctions macrophages, unlike...
Context: Dipeptidyl peptidase 4 (CD26/DPP4) is expressed on blood T cells and also circulates in a soluble form (sCD26/DPP4). Objective: We aimed to evaluate cell circulating CD26/DPP4 its association with metabolic parameters patients type 2 diabetes mellitus (T2DM). Designs: measured expression (percentage of CD26+ using flow cytometry) CD4+ CD8+ cells, serum level activity, various T2DM not DPP4 inhibitor therapy (n = 148). Nondiabetic subjects 50) were included as control group. Results:...
Abstract The integrity of stratified epithelia depends on the ability progenitor cells to maintain a balance between proliferation and differentiation. While much is known about transcriptional pathways underlying cells’ behavior in epidermis, role posttranscriptional regulation by mRNA binding proteins—a rate-limiting step sculpting proteome—remains poorly understood. Here we report that RNA protein YBX1 (Y-box protein-1) critical effector progenitors’ function epidermis. expression...
Abstract Metformin is the primary treatment for type 2 diabetes mellitus (T2DM) due to its effectiveness in improving clinical outcomes patients with preserved renal function, however, evidence on of metformin various functions lacking. We performed a retrospective, multicenter, observational study used data T2DM obtained from three tertiary hospitals’ databases. Patients given within run-in periods and at least one additional prescription formed cohort. A control cohort comprised those...
L. has been widely used by humans for centuries various purposes, such as industrial, ceremonial, medicinal, and food. The bioactive components of
Highlights•The cen and ik2 cis-NAT mRNAs co-localize to centrosomes in Drosophila embryos•Loss of disrupts centrosomal localization, revealing a targeting co-dependency•Centrosomal co-localization requires interactions between the 3′ UTRs ik2•Subcellular fractionation data reveal that cis-NATs often localize similar sitesSummaryOverlapping genes are prevalent most genomes, but extent which this organization influences regulatory events operating at post-transcriptional level remains unclear....
Recently, cannabidiol (CBD), one of the major components Cannabis species, has been a focus in cannabis industry due to its various pharmacological effects. Interestingly, CBD can be converted into several psychoactive cannabinoids, such as 9-tetrahydrocannabinol (Δ9-THC) and structural isomers, under acidic reaction conditions. In this study, chemical transformation ethanol solution was conducted with variation pH at 2.0, 3.5, 5.0 by addition 0.1 M hydrochloric acid (HCl). These resulting...
Coronavirus disease 2019 (COVID-19) has had a major impact on global communities and healthcare.Paxlovid (nirmatrelvir/ritonavir) is an oral drug for the treatment of COVID-19 that been approved by U.S. Food Drug Administration.In Korea, Paxlovid first-line patients who are >60 years old or using immunosuppressants, those >40 with chronic diseases such as obesity, diabetes, kidney disease, hypertension.One caveat potential risk interaction it strong cytochrome P450 (CYP) 3A P-glycoprotein...
While numerous studies have described the transcriptomes of extracellular vesicles (EVs) in different cellular contexts, these efforts typically relied on sequencing methods requiring RNA fragmentation, which limits interpretations integrity and isoform diversity EV-targeted populations. It has been assumed that mRNA signatures EVs are likely to be fragmentation products material, extent full-length mRNAs present within remains clarified.Using long-read nanopore sequencing, we sought...
The Drosophila raf (D-raf) gene promoter contains a recognition consensus sequence for STAT (D-STAT). By band mobility shift assay, we detected factor binding to the D-STAT-recognition in extracts of cultured cells treated with vanadate peroxide. UV-cross-linking analyses suggested size be almost same as that D-STAT. Furthermore, activity was increased cotransfected HOP and D-STAT expression plasmids. These results strongly suggest binds D-raf promoter. Transient luciferase assay using...
The DRE/DREF system plays an important role in transcription of DNA replication genes such as those encoding the 180 and 73 kDa subunits polymerase α well that for PCNA.In this study, we found two sequences homologous to DRE (5′-TATCGATA-3′) 5′-flanking region (-370 -357 with respect initiation site) D-raf gene confirmed transcriptional activity through gel mobility shift assays, transient CAT spatial patterns lacZ expression transgenic larval tissues carrying fusion genes.Further,...
dREAM complexes represent the predominant form of E2F/RBF repressor in Drosophila.dREAM associates with thousands sites fly genome but its mechanism action is unknown.To understand genomic context which acts we examined distribution and localization Drosophila E2F proteins.Here report a striking unexpected overlap between dE2F2/dREAM binding for insulator-binding proteins CP190 Beaf-32.Genetic assays show that these components functionally co-operate chromatin immunoprecipitation experiments...
Lipopolysaccharide (LPS)-induced neuroinflammation is a well-established model for studying depression-like behavior, driven by pro-inflammatory cytokines such as TNF-α and IL-1β. Mast cells (MCs) contribute to releasing mediators that exacerbate depressive-like symptoms. This study evaluates the antidepressant-like anti-inflammatory effects of
Interleukin 6 (IL6) is a pleiotropic cytokine that not only affects the immune system, but also plays an active role in many physiological events various organs. Notably, 35% of systemic IL6 originates from adipose tissues under noninflammatory conditions. Here, we describe previously unknown function melanocortins regulating Il6 gene expression and production 3T3-L1 adipocytes through membrane receptors which are called melanocortin (MCRs). Of five MCRs have been cloned, MC2R MC5R expressed...
The DNA replication‐related element binding factor (DREF) has been suggested as being involved in regulation of replication‐ and proliferation‐related genes Drosophila . Recently, by searching the genome database, we also found DRE‐like sequences 5′‐flanking regions many with other functions. In addition, immunostaining polytene chromosomes an anti‐DREF monoclonal antibody revealed that DREF can bind to a hundred chromosomes, suggesting multiple roles vivo When over‐expressed protein or...
The gene promoter of Drosophila proliferating cell nuclear antigen ( dPCNA ) contains several transcriptional regulatory elements, such as upstream element (URE), DNA replication‐related (DRE, 5′‐TATCGATA), and E2F recognition sites. In the present study, a yeast one‐hybrid screen using three tandem repeats DRE in bait allowed isolation cDNA encoding Cut, homolog mammalian CCAAT‐displacement protein (CDP)/Cux. Electrophoretic mobility shift assays showed that Cut bound to both sequence...
Aberrations of Notch signaling have been implicated in a variety human cancers. Oncogenic mutations NOTCH1 are common T-cell leukemia and lymphomas. However, loss-of-function somatic arising solid tumors imply tumor suppressor function, which highlights the need to understand more completely. Here, we describe small GTPase RhoE/Rnd3 as downstream mediator squamous cell carcinomas (SCC) that arise skin epithelia. RhoE is transcriptional target activated Notch1, attenuated broadly SCC cells....
Peroxisome proliferator-activated receptor gamma (PPARγ) agonists have anti-inflammatory properties that reduce inflammatory cytokine production in rheumatoid arthritis (RA). Cysteine-rich angiogenic inducer 61 (Cyr61) is associated with diseases related to chronic inflammation. The aim of this study was investigate the mechanisms underlying effects PPARγ on tumor necrosis factor (TNF)-α-induced fibroblast-like synoviocyte (FLS) invasion and migration, as well Cyr61 production, RA-FLS. FLS...
Immunoglobulin M (IgM) nephropathy (IgMN) is characterized by the IgM deposition in kidney's mesangium. We assessed impact of electron-dense deposits (EDDs) on IgMN and compared it to other kidney diseases. enrolled 63 adult patients with who underwent renal biopsy from May 2003 June 2017. clinicopathological features based EDD presence; characteristics 91 minimal change disease (MCD), 103 focal segmental glomerulosclerosis (FSGS), 469 immunoglobulin A (IgAN) patients. Renal events were...
We developed a 3D glomeruli tissue chip for glomerulonephritis (GN) testing, featuring gravity-driven glomerular filtration barrier (GFB) with human podocytes and endothelial cells bidirectional flow in the bottom channel. Using puromycin-induced GN, we observed decreased cell viability, increased albumin permeability, reduced WT1 nephrin compared to normal GFB. Tacrolimus restored expression. serum from five membranous nephropathy (MN) patients, created MN models using GFB-mimicking chip. A...
Glomerulonephritis (GN) is characterized by podocyte injury or glomerular filtration dysfunction, which results in proteinuria and eventual loss of kidney function. Progress studying the mechanism GN, developing an effective therapy, has been limited absence suitable vitro models that can closely recapitulate human physiological responses. We developed a microfluidic glomerulus-on-a-chip device environment to construct functional barrier, with we investigated biological changes podocytes...