A5052070727- CRISPR and Genetic Engineering
- RNA Research and Splicing
- RNA modifications and cancer
- Genetic Neurodegenerative Diseases
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- COVID-19 Clinical Research Studies
- Cancer-related gene regulation
- Data-Driven Disease Surveillance
- RNA regulation and disease
- Pancreatic function and diabetes
- Genomics and Chromatin Dynamics
- Long-Term Effects of COVID-19
- Pluripotent Stem Cells Research
- Protein Degradation and Inhibitors
- Muscle Physiology and Disorders
- COVID-19 and healthcare impacts
- COVID-19 epidemiological studies
- Mitochondrial Function and Pathology
- Dermatological and COVID-19 studies
- SARS-CoV-2 detection and testing
- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- Autophagy in Disease and Therapy
- Chromosomal and Genetic Variations
Harvard University
2023-2024
Rockefeller University
2024
Max Planck Institute for Biology of Ageing
2024
GlaxoSmithKline (India)
2024
University of California, San Diego
2015-2022
Dermatology Specialists
2021
University of California San Diego Medical Center
2021
Sanford Consortium for Regenerative Medicine
2021
National Cancer Institute
2015
Center for Cancer Research
2015
Abstract Huntington’s disease (HD) is a fatal, dominantly inherited neurodegenerative disorder caused by CAG trinucleotide expansion in exon 1 of the huntingtin ( HTT ) gene. Since reduction pathogenic mutant messenger RNA therapeutic, we developed allele-sensitive EX RNA-targeting CRISPR–Cas13d system (Cas13d–CAG that eliminates toxic fibroblasts derived from patients with HD and induced pluripotent stem cell-derived neurons. We show intrastriatal delivery Cas13d–CAG via an adeno-associated...
Site-directed RNA editing approaches offer great potential to correct genetic mutations in somatic cells while avoiding permanent off-target genomic edits. Nuclease-dead RNA-targeting CRISPR-Cas systems recruit functional effectors molecules a programmable fashion. Here, we demonstrate Streptococcus pyogenes Cas9-ADAR2 fusion system that uses 3′ modified guide (gRNA) enable adenosine-to-inosine (A-to-I) of specific bases on reporter and endogenously expressed mRNAs. Due the sufficient nature...
The twenty-three Fanconi anemia (FA) proteins cooperate in the FA/BRCA pathway to repair DNA interstrand cross-links (ICLs). cell division cycle and apoptosis regulator 1 (CCAR1) protein is also a of ICL repair, though its possible function remains unknown. Here, we demonstrate that CCAR1 plays unique upstream role required for FANCA expression human cells. Interestingly, co-immunoprecipitates with pre-mRNA mRNA processing. Loss results retention poison exon transcript, thereby leading...
Abstract RNA binding proteins (RBPs) are critical regulators of gene expression and processing that required for function. Yet, the dynamics RBP regulation in single cells is unknown. To address this gap understanding, we developed STAMP (Surveying Targets by APOBEC Mediated Profiling), which efficiently detects RBP-RNA interactions. does not rely on UV-crosslinking or immunoprecipitation and, when coupled with single-cell capture, can identify RBP- cell type-specific RNA-protein...
Abstract Huntington’s disease (HD) is a fatal, dominantly inherited neurodegenerative disorder caused by CAG trinucleotide expansion in exon 1 of the huntingtin ( HTT ) gene. Although pathogenesis HD remains complex, CAG-expanded (CAG EX mRNA and protein ultimately causes through toxic gain-of-function mechanism. As reduction pathogenic mutant beneficial as treatment, we developed RNA-eliminating CRISPR-Cas13d system (Cas13d/CAG that binds eliminates RNA patient iPSC-derived striatal...
Nutrient deficiency during pregnancy in numerous animal species can induce the state of embryonic diapause. Diapause is characterized by changes protein and gene expression that minimize organism's reliance on external energy sources ensure survival. Remarkably, systematic associated with diapause appear to spare program supports cells' maintenance pluripotent state. The phenomenon differentiation "freeze" be reproduced
Abstract Background Successful containment strategies for SARS-CoV-2, the causative virus of COVID-19 pandemic, have involved widespread population testing that identifies infections early and enables rapid contact tracing. In this study, we developed a inexpensive RT- qPCR pipeline population-level SARS-CoV-2 detection, used to establish clinical laboratory dedicated at University California San Diego (UCSD) with processing capacity 6,000 samples per day next-day result turnaround times....
Background: Successful containment strategies for SARS-CoV-2, the causative virus of COVID-19 pandemic, have involved widespread population testing that identifies infections early and enables rapid contact tracing.In this study, we developed a inexpensive RT-qPCR pipeline population-level SARS-CoV-2 detection, used to establish clinical laboratory dedicated at University California San Diego (UCSD) with processing capacity 6,000 samples per day next-day result turnaround times.Methods...
Abstract Exposure of unicellular or multicellular organisms to adverse environmental conditions, including nutrient deprivation, may induce a state suspended animation diapause. The diapause minimizes the organism’s reliance on external energy sources and ensures survival. Among different forms diapause, embryonic caused by limited supply nutrients growing embryos, is particularly challenging for organism. Diapause embryos stop developing at peak pluripotent cell differentiation maintain...