A5077164595- Angiogenesis and VEGF in Cancer
- Renal Transplantation Outcomes and Treatments
- Mesenchymal stem cell research
- Lymphatic System and Diseases
- Hematopoietic Stem Cell Transplantation
- Organ Transplantation Techniques and Outcomes
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Cancer, Hypoxia, and Metabolism
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- Neonatal Respiratory Health Research
- Nitric Oxide and Endothelin Effects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Organ Donation and Transplantation
- Erythrocyte Function and Pathophysiology
- SARS-CoV-2 detection and testing
- Neurofibromatosis and Schwannoma Cases
- Congenital Diaphragmatic Hernia Studies
- SARS-CoV-2 and COVID-19 Research
- Zebrafish Biomedical Research Applications
- Dietary Effects on Health
- Inflammatory Biomarkers in Disease Prognosis
Scripps Health
2020-2022
Scripps Clinic
2017-2022
Scripps Green Hospital
2017-2021
Scripps Clinic Medical Group
2015-2020
Indiana University Health
2010-2018
Indiana University – Purdue University Indianapolis
2009-2018
Scripps (United States)
2018
Scripps Institution of Oceanography
2018
Indiana University School of Medicine
2008-2017
Indiana University
2008-2016
Objective— We sought to identify and characterize 2 distinct populations of bona fide circulating endothelial cells, including the colony-forming cell (ECFC), by polychromatic flow cytometry (PFC), colony assays, immunomagnetic selection, electron microscopy. Methods Results— Mononuclear cells from human umbilical cord blood peripheral were analyzed using our recently published PFC protocol. A population containing both ECFCs mature was determined varying expressions CD34, CD31, CD146 but...
An assay for circulating cell subsets in human peripheral blood by flow cytometry is used as a biomarker to determine cardiovascular disease risk and tumor responsiveness chemotherapy since endothelial progenitor cells (EPCs) function vasculogenesis angiogenesis. Despite analytical advances polychromatic (PFC), conventional approaches are routinely utilized enumerate isolate EPCs, which has led varied results clinical studies, potential cellular misidentification, thus lack of plausible...
Defining whether human circulating proangiogenic cells represent a subset of the hematopoietic system and express CD45 or are derivatives that do not (and called endothelial progenitor cells) remains controversial. We have previously developed polychromatic flow cytometry (PFC) protocol to isolate subsets we now identify pool CD34(+)CD45(dim) representing functional stem (CHSPCs) can be separated on basis AC133 expression report AC133(+) CHSPCs enhances growth tumor blood vessels in vivo...
Electroacupuncture (EA) performed in rats and humans using limb acupuncture sites, LI-4 LI-11, GV-14 GV-20 (humans) Bai-hui (rats) increased functional connectivity between the anterior hypothalamus amygdala mobilized mesenchymal stem cells (MSCs) into systemic circulation. In human subjects, source of MSC was found to be primarily adipose tissue, whereas rodents tissue sources were considered more heterogeneous. Pharmacological disinhibition rat enhanced sympathetic nervous system (SNS)...
Since the initial identification of novel coronavirus SARS-CoV-2 in December 2019, researchers have raced to understand its pathogenesis and begun devising vaccine treatment strategies. An accurate understanding body’s temporal immune response against is paramount successful development disease progression monitoring. To provide insight into antibody SARS-CoV-2, plasma samples from 181 PCR-confirmed COVID-19 patients collected at various timepoints post-symptom onset (PSO) were tested for...
Preventing pathological ocular angiogenesis is key to treating retinopathy of prematurity, diabetic and age-related macular degeneration. At present there no small molecule drug on the market target this process hence a pressing need for developing novel molecules that can replace or complement surgical biologic therapies these neovascular eye diseases. Previously, an antiangiogenic homoisoflavanone was isolated from bulb medicinal orchid, Cremastra appendiculata. In study, we synthesis...
A bstract : Pluripotent stem cells (PSCs) with transdifferentiation capacity may provide useful therapeutic modalities in the areas of cellular restoration and regenerative medicine. The utility PSCs depends on their ability to respond different stimuli adapt tissue‐specific differentiation conditions. Given that a number possessing characteristics have been identified isolated from several adult murine tissues, we hypothesized common PSC exist multiple tissues these either reside...
Background— Neurofibromatosis type 1 (NF1) is a genetic disorder resulting from mutations in the NF1 tumor suppressor gene. Neurofibromin, protein product of , functions as negative regulator Ras activity circulating hematopoietic and vascular wall cells, which are critical for maintaining vessel homeostasis. patients have evidence chronic inflammation development premature cardiovascular disease, including arterial aneurysms, may manifest sudden death. However, molecular pathogenesis...
Transplantation of liver grafts from donation after cardiac death (DCD) is limited. To identify barriers DCD utilization, all active US transplant centers (n = 138) were surveyed, and the responses compared with United Network for Organ Sharing (UNOS) data. In total, 74 (54%) responded, diversity in attitudes was observed, many not using organ and/or recipient prognostic variables defined prior studies UNOS data analysis. Most (74%) believed lack a system allowing timely retransplant barrier...
Rationale: Transmembrane tumor necrosis factor-α (tmTNF-α) is the prime ligand for TNF receptor 2, which has been shown to mediate angiogenic and blood vessel repair activities in mice. We have previously reported that potential of highly proliferative endothelial colony–forming cells (ECFCs) can be explained by absence senescent cells, mature occupy >30% population, exposure a chronic inflammatory environment induced premature, telomere-independent senescence ECFCs. Objective: The goal...
The purpose of this study was to determine the effects in vivo estrogen manipulations on mechanisms endothelium-dependent vasodilation. Ovary-intact, ovariectomized (OVX), or OVX with replacement (OVX + E(2)) female Sprague-Dawley rats were studied (n = 8). Mesenteric arteries (approximately 300 microm) isolated, cannulated, and pressurized 60 mm Hg an arteriograph containing bicarbonate buffer vessel diameter monitored. Concentration-response curves histamine H(1) agonist...