A5084756322- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Gender Diversity and Inequality
- RNA and protein synthesis mechanisms
- Diversity and Career in Medicine
- Advanced Data Storage Technologies
- COVID-19 and healthcare impacts
- COVID-19 diagnosis using AI
- Data-Driven Disease Surveillance
- Career Development and Diversity
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- Cell Image Analysis Techniques
- Evolution and Genetic Dynamics
- Electrowetting and Microfluidic Technologies
- SARS-CoV-2 detection and testing
- Sex and Gender in Healthcare
- Microfluidic and Capillary Electrophoresis Applications
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- CRISPR and Genetic Engineering
University of California, San Diego
2020-2024
Sanford Consortium for Regenerative Medicine
2021
QB3
2016-2017
University of California, San Francisco
2016-2017
Quantitative BioSciences
2017
University of California System
2017
Abstract The spatial organization of molecules in a cell is essential for their functions. While current methods focus on discerning tissue architecture, cell–cell interactions, and expression patterns, they are limited to the multicellular scale. We present Bento, Python toolkit that takes advantage single-molecule information enable analysis at subcellular Bento ingests molecular coordinates segmentation boundaries perform three analyses: defining domains, annotating localization...
Abstract The spatial organization of molecules in a cell is essential for performing their functions. Spatial transcriptomics technologies have opened the door to characterization cellular and subcellular organization. While current computational methods focus on discerning tissue architecture, cell-cell interactions expression patterns, these approaches are limited investigating variation at multicellular scale. We present Bento, Python toolkit that fully takes advantage single-molecule...
We describe a simple bulk method to prepare double emulsions for high-throughput analysis and sorting with flow cytometers.
Microfluidic devices are valuable for a variety of biotechnology applications, such as synthesizing biochemical libraries, screening enzymes, and analyzing single cells. However, normally, the controlled using specialized pumps, which require expert knowledge to operate. Here, we demonstrate operation poly(dimethylsiloxane) without pumps. We build scaffold that holds device reagents be infused in format can inserted into 50 mL falcon tube spun common lab centrifuge. By controlling design...
A specimen to data tracking tool for SEARCH SARS-CoV-2 tests. The application is used by technicians track samples as they proceed through each step within the RT-qPCR testing workflow. app currently hosted here: http://inspect-covid.com/qpcr_records/
Abstract Background Successful containment strategies for SARS-CoV-2, the causative virus of COVID-19 pandemic, have involved widespread population testing that identifies infections early and enables rapid contact tracing. In this study, we developed a inexpensive RT- qPCR pipeline population-level SARS-CoV-2 detection, used to establish clinical laboratory dedicated at University California San Diego (UCSD) with processing capacity 6,000 samples per day next-day result turnaround times....
Background: Successful containment strategies for SARS-CoV-2, the causative virus of COVID-19 pandemic, have involved widespread population testing that identifies infections early and enables rapid contact tracing.In this study, we developed a inexpensive RT-qPCR pipeline population-level SARS-CoV-2 detection, used to establish clinical laboratory dedicated at University California San Diego (UCSD) with processing capacity 6,000 samples per day next-day result turnaround times.Methods...
Disparities for women and minorities in science, technology, engineering, math (STEM) careers have continued even amidst mounting evidence the superior performance of diverse workforces. In response, we launched Diversity Science Lecture series, a cross-institutional platform where junior life scientists present their research comment on diversity, equity, inclusion STEM. We characterize speaker representation from 79 profiles investigate topic noteworthiness via quantitative content...
Abstract We present Bento, a Python toolkit for subcellular spatial analysis of RNA, implementing tools ingesting highly-multiplexed transcriptomics data, visualization, detection RNA localization patterns, identification cell states, and statistics. applied our methods to analyze multiple datasets from variety platforms, including seqFISH+ (10k in cultured mouse fibroblast cells) MERFISH (130 U2-OS cells), the Spatial Molecular Imager (980 FFPE NSCLC breast cancer tumors) show how can be...
Abstract Disparities for women and minorities in science, technology, engineering, math (STEM) careers have continued even amidst mounting evidence the superior performance of diverse workforces. In response, we launched Diversity Science Lecture series, a cross-institutional platform where junior life scientists present their research comment on equity, diversity, inclusion STEM. We characterize speaker representation from 79 profiles investigate topic noteworthiness via quantitative...