A5013598446- Gut microbiota and health
- Diet and metabolism studies
- Nutritional Studies and Diet
- Malaria Research and Control
- Complement system in diseases
- Clostridium difficile and Clostridium perfringens research
- HIV Research and Treatment
- Obesity, Physical Activity, Diet
- Physical Activity and Health
- Pediatric health and respiratory diseases
- Diversity and Career in Medicine
- Gender Diversity and Inequality
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Dental Research and COVID-19
- Tea Polyphenols and Effects
- Eating Disorders and Behaviors
- Tryptophan and brain disorders
- Mosquito-borne diseases and control
- Sex and Gender in Healthcare
- Infection Control and Ventilation
- Vibrio bacteria research studies
- Therapeutic Uses of Natural Elements
- Heavy Metal Exposure and Toxicity
- Fecal contamination and water quality
- Computational Drug Discovery Methods
University of California, San Diego
2020-2025
Scripps Institution of Oceanography
2020-2024
University of Ghana
2017-2019
University of Copenhagen
2017-2019
Noguchi Memorial Institute for Medical Research
2017-2019
Rigshospitalet
2017
Copenhagen University Hospital
2017
The relationship between microbiota, short chain fatty acids (SCFAs), and obesity remains enigmatic. We employ amplicon sequencing targeted metabolomics in a large (n = 1904) African origin cohort from Ghana, South Africa, Jamaica, Seychelles, the US. Microbiota diversity fecal SCFAs are greatest Ghanaians, lowest Americans, representing each end of urbanization spectrum. Obesity is significantly associated with reduction SCFA concentration, microbial diversity, synthesizing bacteria,...
Cerebral malaria is a deadly outcome of infection by Plasmodium falciparum, occurring when parasite-infected erythrocytes accumulate in the brain. These display parasite proteins PfEMP1 family that bind various endothelial receptors. Despite importance cerebral malaria, binding phenotype linked to its symptoms has not been identified. Here, we used structural biology determine how group interacts with intercellular adhesion molecule 1 (ICAM-1), allowing us predict binders from specific...
SARS-CoV-2 is an RNA virus responsible for the coronavirus disease 2019 (COVID-19) pandemic. Viruses exist in complex microbial environments, and recent studies have revealed both synergistic antagonistic effects of specific bacterial taxa on viral prevalence infectivity. We set out to test whether communities predict occurrence a hospital setting.We collected 972 samples from hospitalized patients with COVID-19, their health care providers, surfaces before, during, after admission. screened...
Abstract Chronic dietary protein-restriction can create essential amino acid deficiencies and induce metabolic adaptation through the hepatic FGF21 pathway which serves to maintain host fitness during prolonged states of nutritional imbalance. Similarly, gut microbiome undergoes adaptations when nutrients are added or withdrawn. Here we confirm previous reports that triggers adaptive further demonstrate this response is mediated by be tuned supplementation fibers alter microbiome. In absence...
Traditional obesity-related public health messaging often includes physical activity (PA) recommendations. However, at the population level, data are conflicting, especially when comparing different self-reported vs. measured techniques across settings and populations. We association between moderate-to-vigorous intensity PA (MVPA) prospective weight change five African-origin populations extent to which MVPA attenuated over time. At baseline, 2,500 adults (median age: 37y) were recruited...
PfEMP1 is a family of adhesive proteins expressed on the surface Plasmodium falciparum-infected erythrocytes (IEs), where they mediate adhesion IEs to range host receptors. Efficient PfEMP1-dependent IE sequestration often depends soluble serum proteins, including IgM. Here, we report comprehensive investigation which about 60 var gene-encoded variants per parasite genome can bind IgM via Fc part antibody molecule, and constituent domains those are involved. We erased epigenetic memory gene...
Cerebral malaria (CM) is a potentially deadly outcome of
erythrocyte membrane protein 1 (PfEMP1) is an important malaria virulence factor. The family can be divided into clinically relevant subfamilies. ICAM-1-binding group A PfEMP1 proteins also bind endothelial C receptor and have been associated with cerebral in children. IgG to these acquired later life than that not binding ICAM-1. kinetics of acquisition B ICAM-1 unclear was studied here. Gene sequences encoding DBLβ domains known were used identify additional binders. Levels specific for...
Synergistic effects of bacteria on viral stability and transmission are widely documented but remain unclear in the context SARS-CoV-2. We collected 972 samples from hospitalized ICU patients with coronavirus disease 2019 (COVID-19), their health care providers, hospital surfaces before, during, after admission. screened for SARS-CoV-2 using RT-qPCR, characterized microbial communities 16S rRNA gene amplicon sequencing, contextualized massive diversity this dataset a meta-analysis over...
Fermented foods are becoming more popular due to their purported links metabolic health and the gut microbiome. However, direct clinical evidence for claims is lacking. Here, we describe an eight-week trial that explored effects of a four-week kombucha supplement in healthy individuals consuming Western diet, randomized into (n = 16) or control 8) group. We collected longitudinal stool blood samples profile human microbiome inflammation markers. did not observe significant changes either...
The lack of suitable animal models for the study cytoadhesion P. falciparum-infected erythrocytes (IEs) has necessitated in vitro studies employing a range cell lines either human tumour origin (e.g., BeWo and C32 cells) or non-human CHO cells). Of cells available, many were isolated from adults, derived pool donors HBEC-5i). Here we demonstrate, first time, successful isolation blood outgrowth endothelial (BOECs) frozen stabilates peripheral mononuclear obtained small-volume samples...
Abstract Fermented foods are surging in popularity globally due to their links metabolic health and the gut microbiome. However, direct clinical evidence for claims is lacking. Here, we describe an eight-week trial that explored effects of a kombucha supplement healthy individuals consuming Western diet, randomized into ( n = 16) or control 8) group. We collected longitudinal stool blood samples profile human microbiome inflammation markers. Paired analysis between baseline end intervention...
Abstract Background Human milk is unquestionably beneficial for preterm infants. We investigated how the transition from tube to oral/breastfeeding impacts infants’ oral and gut microbiome metabolome. Methods analyzed stool, saliva, samples collected a cohort of infants enrolled in MAP Study, prospective observational trial. The metabolome were 4 longitudinal sample time points, 2 during feeds only after initiation oral/breastfeeding. Results 11 mother-infant dyads (gestational age = 27.9...
Abstract Disclosure: J. Jorgensen: None. C. Choo-Kang: L. Issa: J.A. Gilbert: G. Ecklu-Mensah: A. Luke: K. Bedu-Addo: T. Forrester: P. Bovet: E.V. Lambert: D. Rae: M. Argos: Y. Dai: R.M. Sargis: L.R. Dugas: B.T. Layden: Obesity is associated with an increased risk for cardiometabolic diseases, such as type 2 diabetes mellitus (T2D). Prior research has shown association between toxic metals/metalloid (hereafter, “metals”) exposures, including arsenic (As), lead (Pb), mercury (Hg), and cadmium...
ABSTRACT Exposure to toxic metals impacts obesity and type 2 diabetes (T2DM) risk. Yet, the underlying mechanisms remain largely unknown. Gut microbiota has been strongly associated with progression of cardiometabolic To determine whether high metal exposures gut dysbiosis interact promote metabolic dysregulation risk, we assessed relationships between these factors. We analyzed cross-sectional associations arsenic, lead, mercury, cadmium, health markers in 178 randomly selected...
<title>Abstract</title> <bold>Background:</bold> Physical activity (PA) is an important preventive factor of non-communicable diseases (NCDs), particularly cardiovascular disease, yet progress towards reducing physical inactivity in populations slow. Population-levels PA are most often estimated using self-report questionnaires population surveys, such as the Global Questionnaire (GPAQ), which may not accurately reflect objectively measured PA, accelerometers. The aim current study was to...
<title>Abstract</title> Traditional obesity-related public health messaging often includes physical activity (PA) recommendations. However, at the population level, data are conflicting, especially when comparing different self-reported vs measured techniques across settings and populations. We association between moderate-to-vigorous intensity PA (MVPA) prospective weight change five African-origin populations extent to which MVPA attenuated over time. At baseline, 2,500 adults (median age:...
The relationship between the gut microbiota, short chain fatty acid (SCFA) metabolism, and obesity remains unclear due to conflicting reports from studies with limited statistical power. Additionally, this association has rarely been explored in large scale diverse populations. Here, we investigated associations fecal microbial composition, predicted metabolic potential, SCFA concentrations, a ( N = 1,934) adult cohort of African-origin spanning epidemiologic transition, Ghana, South Africa,...
Abstract Disclosure: J. Jorgensen: None. C. Choo-Kang: G. Ecklu-Mensah: Gilbert: A. Luke: K. Bedu-Addo: T.E. Forrester: P. Bovet: E.V. Lambert: D. Rae: L. Dugas: Y. Dai: B.T. Layden: Obesity is a global public health crisis, which since 1975 has tripled world-wide. Concomitantly, obesity-related conditions such as cardiovascular disease, stroke, and type 2 diabetes have also increased. An accepted common obesogenic factor been shift away from fiber-rich diets to ultra-processed foods. Fiber...