A5022455904- Neurotransmitter Receptor Influence on Behavior
- Sleep and Wakefulness Research
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Regulation of Appetite and Obesity
- Neurological disorders and treatments
- Sleep and related disorders
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Stress Responses and Cortisol
- Neural dynamics and brain function
- Attention Deficit Hyperactivity Disorder
- Functional Brain Connectivity Studies
- Insect symbiosis and bacterial influences
- Neuropeptides and Animal Physiology
- Neuroscience, Education and Cognitive Function
- Insect Utilization and Effects
- Hematopoietic Stem Cell Transplantation
- Neural and Behavioral Psychology Studies
- Heart Rate Variability and Autonomic Control
- Visual perception and processing mechanisms
- Neuroendocrine regulation and behavior
- Hydrogen's biological and therapeutic effects
- Olfactory and Sensory Function Studies
- Zebrafish Biomedical Research Applications
Université Libre de Bruxelles
2015-2024
Neuroscience Institute
2022
Institut de Psychiatrie et Neurosciences de Paris
2018
Stanford University
2010-2017
Sorbonne Université
2003-2012
Centre National de la Recherche Scientifique
2003-2012
Institut du Cerveau
2012
Inserm
2006-2011
Stanford Medicine
2010
Institut Jacques Monod
2003
Current models of sleep/wake regulation posit that Hypocretin (Hcrt)-expressing neurons in the lateral hypothalamus promote and stabilize wakefulness by projecting to subcortical arousal centers. However, critical downstream effectors Hcrt are unknown. Here we use optogenetic, pharmacological, computational tools investigate functional connectivity between noradrenergic locus coeruleus (LC) during nonrapid eye movement (NREM) sleep. We found photoinhibiting LC stimulation blocked...
Abstract The hypothalamic–pituitary–adrenal (HPA) axis functions to coordinate behavioural and physiological responses stress in a manner that depends on the state of organism. However, mechanisms through which arousal metabolic states influence HPA are poorly understood. Here using optogenetic approaches mice, we show neurons produce hypocretin (Hcrt)/orexin lateral hypothalamic area (LHA) regulate corticosterone release variety behaviours hallmarks response. Interestingly, found Hcrt...
The basal ganglia are known to control actions and modulate movements. Neuronal activity in the two efferent pathways of dorsal striatum is critical for appropriate behavioral control. Previous evidence has led divergent conclusions on respective engagement both during actions. Using calcium imaging evaluate how neurons direct indirect encode behaviors self-paced spontaneous explorations an open field, we observed that striatal exhibit distinct tuning properties. Supervised learning...
Histaminergic (HA) neurons, found in the posterior hypothalamic tuberomammillary nucleus (TMN), extend fibers throughout brain and exert modulatory influence over numerous physiological systems. Multiple lines of evidence suggest that activity HA neurons is important regulation vigilance despite lack direct, causal demonstrating its requirement for maintenance arousal during wakefulness. Given strong correlation between neuron excitability behavioral arousal, we investigated both...
Drug addiction is a chronic relapsing disorder characterized by compulsive drug seeking and use. Environmental conditioning factors are among the major determinants of relapse in abstinent cocaine users. Here we describe role neuropeptide S (NPS) system regulating relapse. In rats with history self-administration, presentation stimuli predictive availability reinstates seeking, triggering Intracerebroventricular (ICV) injection NPS increased conditioned reinstatement whereas peripheral...
Many of the factors affecting success haematopoietic cell transplantation are still unknown. Here we show in mice that donor sleep deprivation reduces ability its stem cells (HSCs) to engraft and reconstitute blood bone marrow an irradiated recipient by more than 50%. We demonstrate downregulates expression microRNA (miR)-19b, a negative regulator suppressor cytokine signalling (SOCS) genes, which inhibit HSC migration homing. Accordingly, HSCs from sleep-deprived have higher levels SOCS...
The 5-HT(1A) receptor (5-HT(1A)R) plays a key role in the inhibitory influence of serotonin (5-HT) on rapid eye movement (REM) sleep rodents. However, neuronal networks mediating such are mostly unknown, notably mouse. This led us to map 5-HT(1A)R mRNA, by situ hybridization histochemistry (ISHH), and characterize phenotype mRNA-positive neurons dual ISHH combined with immunohistochemistry, throughout mouse rostral brainstem, pivotal region for generation REM cortical activation. mRNA was...
5-HT neurons are topographically organized in the hindbrain, and have been implicated etiology treatment of psychiatric diseases such as depression anxiety. Early studies suggested that raphe were a homogeneous population showing similar electrical properties, feedback inhibition mediated by 5-HT1A autoreceptors. We utilized histochemistry techniques ePet1-eGFP 5-HT1A-iCre/R26R mice to show subpopulation do not express somatodendritic autoreceptor mRNA. In addition, we performed patch-clamp...
Abstract The central function of the striatum and its dopaminergic (DA) afferents in motor control integration cognitive emotional processes is commonly explained by two striatal efferent pathways characterized projection neurons (SPNs) expressing DA D1 receptors D2 (D1-SPNs D2-SPNs), without regard to SPNs coexpressing both (D1/D2-SPNs). We developed an approach that enables targeting these hybrid demonstrated although are less abundant, they play a major role guiding other main...
Agonists at G-protein-coupled receptors in neurons of the dorsal raphe nucleus (DRN) knock-out mice devoid serotonin transporter (5-HTT−/−) exhibit lower efficacy to inhibit cellular discharge than wild-type counterparts. Using patch-clamp whole-cell recordings, we found that a G-protein-gated inwardly rectifying potassium (GIRK) current is involved inhibition spike induced by 5-HT1A agonists (5-carboxamidotryptamine (5-CT) and (±)-2-dipropylamino-8-hydroxy-1,2,3,4-tetrahydronaphthalene...
Serotonin (5-HT) neurons are involved in wake promotion and exert a strong inhibitory influence on rapid eye movement (REM) sleep. Such effects have been ascribed, at least part to the action of 5-HT post-synaptic 5-HT1A receptors (5-HT1AR) brainstem, major wake/REM sleep regulatory center. However, neuroanatomical substrate through which 5-HT1AR remains elusive. We therefore investigated whether brainstem structure containing high density mRNA, GABAergic Gudden's dorsal tegmental nucleus...
Abstract Prescription stimulants, such as d ‐amphetamine or methylphenidate are used to treat suffering from attention‐deficit hyperactivity disorder (ADHD). They potently release dopamine (DA) and norepinephrine (NE) cause phosphorylation of the α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) receptor subunit GluA1 in striatum. Whether other brain regions also affected remains elusive. Here, we demonstrate that increase at Ser845 (pS845‐GluA1) membrane fraction mouse cerebellum...