A5015316275- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Immune cells in cancer
- Tryptophan and brain disorders
- Alzheimer's disease research and treatments
- Vagus Nerve Stimulation Research
- Chronic Kidney Disease and Diabetes
- Circadian rhythm and melatonin
- Sleep and Wakefulness Research
- Barrier Structure and Function Studies
- Photoreceptor and optogenetics research
- Essential Oils and Antimicrobial Activity
- Renal Diseases and Glomerulopathies
- Renal and Vascular Pathologies
- Acute Ischemic Stroke Management
- Pancreatic function and diabetes
- Plant Pathogenic Bacteria Studies
- Stress Responses and Cortisol
- Cancer Immunotherapy and Biomarkers
- Ion-surface interactions and analysis
- Cardiac Fibrosis and Remodeling
- MRI in cancer diagnosis
- Hematopoietic Stem Cell Transplantation
- Piperaceae Chemical and Biological Studies
Technion – Israel Institute of Technology
2015-2021
Rambam Health Care Campus
2020
Rush University Medical Center
2020
Abstract Regulating immunity is a leading target for cancer therapy. Here, we show that the anti-tumor immune response can be modulated by brain’s reward system, key circuitry in emotional processes. Activation of system tumor-bearing mice (Lewis lung carcinoma (LLC) and B16 melanoma) using chemogenetics (DREADDs), resulted reduced tumor weight. This effect was mediated via sympathetic nervous (SNS), manifested an attenuated noradrenergic input to major immunological site, bone marrow....
Background: Recent evidence suggests that cancer and cardiovascular diseases are associated. Chemotherapy drugs known to result in cardiotoxicity, studies have shown heart failure stress correlate with poor prognosis. However, whether cardiac remodeling the absence of is sufficient promote unknown. Methods: To investigate effect early on tumor growth metastasis colonization, we used transverse aortic constriction (TAC), a model for pressure overload–induced hypertrophy, followed it by cell...
The sympathetic nervous system is composed of an endocrine arm, regulating blood adrenaline and noradrenaline, a local network fibers innervating immune organs. Here, we investigated the impact arm SNS in inflammatory response colon. Intra-rectal insertion optogenetic probe mice engineered to express channelrhodopsin-2 tyrosine hydroxylase cells activated colonic fibers. In contrast systemic application activation attenuated experimental colitis reduced cell abundance. Gene expression...
Macrophages are pleiotropic and diverse cells that populate all tissues of the body. Besides tissue-specific resident macrophages such as alveolar macrophages, Kupffer cells, microglia, multiple organs harbor at least two subtypes other steady state. During certain circumstances, like tissue insult, additional recruited to from monocyte pool. Previously, a macrophage population marked by expression Spp1, Cd9, Gpnmb, Fabp5, Trem2, has been described in several models organ injury cancer,...
Many of the factors affecting success haematopoietic cell transplantation are still unknown. Here we show in mice that donor sleep deprivation reduces ability its stem cells (HSCs) to engraft and reconstitute blood bone marrow an irradiated recipient by more than 50%. We demonstrate downregulates expression microRNA (miR)-19b, a negative regulator suppressor cytokine signalling (SOCS) genes, which inhibit HSC migration homing. Accordingly, HSCs from sleep-deprived have higher levels SOCS...
The activating transcription factor 3 (ATF3) and the c-Jun dimerization protein 2 (JDP2) are members of basic leucine zipper (bZIP) family factors. These proteins share a high degree homology both can activate or repress transcription. Deficiency either one them in non-cancer host cells was shown to reduce metastases. As ATF3 JDP2 compensate each other's function, we studied double deficiency stromal tumor microenvironment. Here, show that mice with (designated thereafter dKO) developed...
ABSTRACT A clinical isolate of Salmonella enterica serovar Senftenberg, isolated from an outbreak linked to the herb Ocimum basilicum L. (basil), has been shown be resistant basil oil and terpene alcohol linalool. To better understand how human pathogens might develop resistance linalool investigate association this with different antimicrobial agents, selective pressure was applied wild-type strain by sequential exposure increasing concentrations The results demonstrated that S ....
Summary Fresh produce contaminated with human pathogens raises vital and ecological questions about bacterial survival strategies. Such occurrence was basil harboring Salmonella enterica serovar Senftenberg that caused an outbreak in 2007. This host unanticipated due to its production of antibacterial substances, including linalool. We show linalool perforates membranes, resulting increased permeability leakage molecules. It also inhibits cell motility causes aggregation. Linalool‐resistance...
Increasing evidence highlight the involvement of immune cells in brain activity and its dysfunction. The brain's compartment is a dynamic ensemble that can fluctuate even naive animals. However, dynamics factors affect composition are largely unknown. Here, we examined whether acute sleep deprivation (parenchyma, meninges, choroid plexus). Using high-dimensional mass cytometry analysis, broadly characterized effects short-term on mouse brain. We found after 6 h deprivation, there was...
Abstract Progesterone has been shown to regulate immunity during pregnancy, and progesterone administration may reduce inflammation-induced preterm labor. We sought determine the maternal brain immune response LPS-induced inflammation in pregnant non-pregnant mice whether additional supplementation attenuates this response. Pregnant (P: n = 9) (NP: were randomized pretreatment with vaginal progesterone/carrier (Replens), daily from days 13 16. On 15 16, LPS/saline was administered by...
Abstract The kidney is a complex organ that governs many physiological parameters. It roughly divided into three parts, the renal pelvis, medulla, and cortex. Covering cortex capsule, serosal tissue provides protection forms barrier for kidney. Serosal tissues of organs have been recently shown to play vital role in homeostasis disease. Analyses cells reside these identified distinct cell types with unique phenotypes. Here, we characterized this niche found it mainly comprised fibroblasts...
Abstract Most causes of chronic kidney disease begin with injury to the glomerulus and involve progressive loss function. The is a capillary bed where blood filtration produce urine in occurs. During aging, there glomeruli capacity because podocytes, glomerular epithelial cell, are lost aging after injury. Podocytes cannot divide therefore be replaced. Our histological analysis confirmed presence glomerulosclerosis, generalized interstitial fibrosis hypertrophy aged mouse kidney. One barrier...
Summary Genetic variations in CD2-associated protein (CD2AP) predispose to Alzheimer’s disease (AD) but the underlying mechanisms remain unknown. Here, we show that a cerebrovascular loss of CD2AP is associated with cognitive decline AD and genetic downregulation brain endothelial cells impairs memory function two distinct mouse models. Mice reduced microvessels display decreased resting cerebral blood flow, impaired functional hyperemia vasomotion. In cells, regulates levels signaling ApoE...
Abstract Increasing evidence indicates that the brain regulates peripheral immunity. Yet, it remains unclear whether and how represents state of immune system. Here, we show immune-related information is stored in brain’s insular cortex (InsCtx). Using activity-dependent cell labeling mice ( Fos TRAP ) , captured neuronal ensembles InsCtx were active under two different inflammatory conditions (DSS-induced colitis Zymosan-induced peritonitis). Chemogenetic reactivation these was sufficient...
Abstract Background Cerebrovascular impairment is a key aspect of Alzheimer’s Disease (AD) pathogenesis. Brain vascular defects appear early in the disease process and are associated with cognitive dysfunction. CD2‐associated protein (CD2AP), scaffolding involved cytoskeleton remodeling membrane trafficking, an important predisposing factor for disease. The enriched brain endothelial cells (BECs) that forms vessels, suggesting CD2AP might contribute to AD cerebrovascular Method In this...
Abstract Recent evidence suggests that the interaction between tumor microenvironment (TME) and systemic host environment can alter immune system to promote anti-tumor activity. Here, we investigated whether glomerular injury affects cancer progression. We used nephrotoxic serum nephritis (NTN), a model for injury, followed it by cell implantation. NTS-injected mice developed smaller primary tumors compared with controls. Tumors of had more activated CD8 T cells, suggesting role in...