A5052253061- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Renal and related cancers
- Renal cell carcinoma treatment
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Cancer Immunotherapy and Biomarkers
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- Viral Infectious Diseases and Gene Expression in Insects
- Asthma and respiratory diseases
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Neonatal Respiratory Health Research
- Cutaneous lymphoproliferative disorders research
- Radiomics and Machine Learning in Medical Imaging
- Dialysis and Renal Disease Management
- Inflammatory Bowel Disease
- Oral and Maxillofacial Pathology
- Chronic Kidney Disease and Diabetes
- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- T-cell and B-cell Immunology
The kinase ITK is required for antigen-stimulated T cell death.
Background Individualized neoantigen-specific immunotherapy (iNeST) requires robustly expressed clonal neoantigens for efficacy, but tumor mutational heterogeneity, loss of neoantigen expression, and variable tissue sampling present challenges. It is assumed that are preferred targets immunotherapy, the distributions not well characterized across cancer types. Methods We combined multiregion sequencing (MR-seq) analysis five untreated, synchronously sampled metastatic solid tumors with...
Organoids are
Optimization and standardization of immunohistochemistry (IHC) protocols within between laboratories requires reproducible positive negative control samples. In many situations, suitable tissue or cell line controls are not available. We demonstrate here a method to incorporate target antigens into synthetic protein gels that can serve as IHC controls. The use peptides, domains, whole proteins antigens, is compatible with variety fixation protocols. resulting be used create microarrays...
Formalin-fixed, paraffin-embedded (FFPE) tissue is the most commonly used material for tumor molecular profiling, therapy selection, and prognostication. Tumor enrichment by dissection highly recommended to generate quality data reproducibly use in downstream assays, such as real-time PCR next-generation sequencing. The aim of this study was evaluate performance automated tool AVENIO Millisect System compared with a manual method using 18 FFPE specimens. assessed these two methods...
Abstract RIT1 is a RAS-family GTPase that mutated in 2.4% and amplified up to 14% of lung adenocarcinoma patients. Yet, the oncogenic potential lungs not completely understood. Many patients with alterations are considered ‘oncogene-negative’, hence they eligible for any targeted therapy clinic. The role cancer has been historically understudied due lack vitro vivo models harboring alterations. In this study, we generated first murine model M90I -mutant cancer. We found was tumorigenic...
Abstract The kidney is a complex organ that governs many physiological parameters. It roughly divided into three parts, the renal pelvis, medulla, and cortex. Covering cortex capsule, serosal tissue provides protection forms barrier for kidney. Serosal tissues of organs have been recently shown to play vital role in homeostasis disease. Analyses cells reside these identified distinct cell types with unique phenotypes. Here, we characterized this niche found it mainly comprised fibroblasts...
With the advent of checkpoint inhibitors, there is increasing need to study dynamics CD8+ T-cells in tumor microenviroment. In this article, we describe a semi-automated method quantify and interrogate spatial relationships between collagenous stroma human mouse tissue samples. The assay combines CD8 immunohistochemistry with modified Masson's trichrome. Slides are scanned digital images analyzed using an adjustable MATLAB algorithm, allowing for high-throughput quantification cytotoxic...
Positive and negative controls with known expression of target proteins are essential for the development immunohistochemistry (IHC) assays. While tissue beneficial well-characterized defined cellular patterns, they less suitable initial IHC assays novel, poorly characterized, or ubiquitously expressed proteins. Alternatively, due to their standardized nature, cell pellets, including cancer lines protein transcript levels (e.g., high, medium, low expression), transfected over-expressing...
Tumor enrichment in low tumor content tissues, those below 20% depending on the method, is required to generate quality data reproducibly with many downstream assays such as next generation sequencing. Automated tissue dissection a new methodology that automates and improves these common, tissues by decreasing user-dependent imprecision of traditional macro-dissection time, cost, expertise limitations laser capture microdissection using digital image annotation overlay onto unstained slides....
Tumor enrichment in low tumor content tissues, those below 20% depending on the method, is required to generate quality data reproducibly with many downstream assays such as next generation sequencing. Automated tissue dissection a new methodology that automates and improves these common, tissues by decreasing user-dependent imprecision of traditional macro-dissection time, cost, expertise limitations laser capture microdissection using digital image annotation overlay onto unstained slides....
Abstract Renal cell carcinoma (RCC) with venous tumor thrombus (VTT) arising from the primary occurs in approximately 10% of cases and is thought to represent more advanced disease. The intravascular nature VTT suggests that it may serve as a source for hematogenous metastases. RCC distant metastasis provides unique opportunities examine origins emergence timing these distinct lesions, identify molecular correlates disease state. We performed multi-region exome RNA-sequencing analysis 16...
Positive and negative controls with known expression of target proteins are essential for the development immunohistochemistry (IHC) assays. While tissue beneficial well-characterized defined cellular patterns, they less suitable initial IHC assays novel, poorly characterized, or ubiquitously expressed proteins. Alternatively, due to their standardized nature, cell pellets, including cancer lines protein transcript levels (e.g., high, medium, low expression), transfected over-expressing...
Abstract Individualized neoantigen specific immunotherapy (iNeST) requires robustly expressed clonal neoantigens for efficacy, but tumor mutational heterogeneity, loss of expression, and variable tissue sampling present challenges. To characterize these potential obstacles, we combined multi-region sequencing (MR-seq) analysis five untreated, synchronously sampled metastatic solid tumors with re-analysis published MR-seq data from 103 patients. Branching evolution in colorectal cancer renal...
Cryopreserving tissues for histology requires the use of coolants to buffer sample from liquid nitrogen (LN2) and control rate temperature decline. Several sharing similar physical characteristics are available on market; however, commonly used variably flammable and/or toxic pose risks personnel facilities. The purpose this study was compare performance three commercially coolants: hexane, 2-methylbutane (2 M), 1-methoxyheptafluoropropane (N7000). Fresh mouse were frozen by each method,...
Mouse models of colitis are used for efficacy testing novel therapeutic compounds. A commonly endpoint studies is the microscopic scoring lesions on whole colon samples, either manually or by automated image analysis platforms. comprehensive system must evaluate complete and continuous mucosal surface entire colon, so areas prone to show pathology visible a single hematoxylin eosin (H&E)-stained slide. Slide preparation requires rolling into Swiss roll configuration in biopsy cassette,...
Supplementary Figure from Origins and Timing of Emerging Lesions in Advanced Renal Cell Carcinoma
Supplementary Figure from Origins and Timing of Emerging Lesions in Advanced Renal Cell Carcinoma
<div>Abstract<p>Renal cell carcinoma (RCC) with venous tumor thrombus (VTT) arising from the primary occurs in approximately 10% of cases and is thought to represent more advanced disease. The intravascular nature VTT suggests that it may serve as a source for hematogenous metastases. RCC distant metastasis provides unique opportunities examine origins emergence timing these distinct lesions, identify molecular correlates disease state. We performed multi-region exome...
Supplementary Data from Origins and Timing of Emerging Lesions in Advanced Renal Cell Carcinoma
Supplementary Data from Origins and Timing of Emerging Lesions in Advanced Renal Cell Carcinoma
<div>Abstract<p>Renal cell carcinoma (RCC) with venous tumor thrombus (VTT) arising from the primary occurs in approximately 10% of cases and is thought to represent more advanced disease. The intravascular nature VTT suggests that it may serve as a source for hematogenous metastases. RCC distant metastasis provides unique opportunities examine origins emergence timing these distinct lesions, identify molecular correlates disease state. We performed multi-region exome...
Supplementary Data from Origins and Timing of Emerging Lesions in Advanced Renal Cell Carcinoma
Supplementary Data from Origins and Timing of Emerging Lesions in Advanced Renal Cell Carcinoma