A5100691294- Immune Cell Function and Interaction
- HIV Research and Treatment
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
- Ovarian cancer diagnosis and treatment
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- HIV-related health complications and treatments
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Spondyloarthritis Studies and Treatments
- Uterine Myomas and Treatments
- Eosinophilic Esophagitis
- Endometrial and Cervical Cancer Treatments
- Reproductive System and Pregnancy
- HIV/AIDS Research and Interventions
- Immune Response and Inflammation
- Endometriosis Research and Treatment
- IL-33, ST2, and ILC Pathways
- Expert finding and Q&A systems
- Liver physiology and pathology
- Bacillus and Francisella bacterial research
Inner Mongolia Agricultural University
2024-2025
Chinese PLA General Hospital
2013-2025
Guizhou Normal University
2025
Jiangnan University
2024
Zhongshan Hospital
2024
Fudan University
2024
Beihang University
2024
Baidu (China)
2023
University of Chinese Academy of Sciences
2022
Chinese People's Liberation Army
2021
In coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome 2 (SARS-CoV-2) infection, the relationship between severity and host immune response is not fully understood. Here we performed single-cell RNA sequencing in peripheral blood samples of 5 healthy donors 13 patients with COVID-19, including moderate, convalescent cases. Through determining transcriptional profiles cells, coupled assembled T cell receptor B sequences, analyzed functional properties cells. Most...
Abstract Programmed death 1 (PD‐1) and its ligand (PD‐L1) play pivotal roles in regulating host immune responses. However, the inhibitory effects of this pathway on function cytotoxic CD8 + T lymphocytes, main effector cells hepatocellular carcinoma (HCC) patients, are not well defined. In study, we characterized circulating intratumor PD‐1/PD‐L1 expression analyzed their association with disease progression a cohort hepatitis B virus‐infected including 56 HCC, 20 liver cirrhosis (LC)...
Abstract COVID-19 is associated with 5.1% mortality. Although the virological, epidemiological, clinical, and management outcome features of patients have been defined rapidly, inflammatory immune profiles require definition as they influence pathogenesis clinical expression COVID-19. Here we show lymphopenia, selective loss CD4+ T cells, CD8+ cells NK excessive T-cell activation high inhibitory molecules are more prominent in severe cases than those mild disease. disease express levels...
Interleukin-17 (IL-17)-producing CD4(+) T cells (Th17)-mediated immune response has been demonstrated to play a critical role in inflammation-associated disease; however, its chronic hepatitis B virus (HBV) infection remains unknown. Here we characterized peripheral and intrahepatic Th17 analyzed their association with liver injury cohort of HBV-infected patients including 66 (CHB), 23 HBV-associated acute-on-chronic failure (ACLF), 30 healthy subjects as controls. The frequency circulating...
SARS-CoV-2 is associated with a 3.4% mortality rate in patients severe disease. The pathogenesis of cases remains unknown. We performed an in-depth prospective analysis immune and inflammation markers two COVID-19 disease from presentation to convalescence. Peripheral blood 18 SARS-CoV-2-infected patients, 9 mild disease, was obtained at admission analyzed for T-cell activation profile, myeloid-derived suppressor cells (MDSCs) cytokine profiles. MDSC functionality tested vitro. In four...
Abstract The role of CD4 + cytotoxic T cells (CTLs) in hepatocellular carcinoma (HCC) remains obscure. This study characterized CTLs HCC patients and further elucidated the associations between disease progression. In all, 547 patients, 44 chronic hepatitis B (CHB) 86 liver cirrhosis (LC) 88 healthy individuals were enrolled study. defined by flow cytometry, immunohistochemistry, lytic granule exocytosis assays. A multivariate analysis prognostic factors for overall survival was performed...
Natural killer (NK) cells are abundant in the liver and serve as a major innate immune component against microbial infection. Although NK have been implicated inducing hepatocellular damage patients with chronic hepatitis virus infections, roles that hepatic play B (HBV) infections remain obscure. In this study, we comprehensively characterized intrahepatic peripheral investigated their impact on pathology cohort of HBV-infected individuals; included 51 immune-activated (IA) patients, 27...
Abstract Although dysfunctional dendritic cells contribute to inadequate adaptive immunity in chronic hepatitis B (CHB), underlying molecular mechanisms remain largely undefined. In this study, we examined B7-H1 expression on circulating myeloid (mDCs) 46 CHB patients, 10 autoimmune and healthy subjects as control. We found that is significantly up-regulated mDCs of patients compared with individuals. The up-regulation was correlated an elevation serum alanine aminotransaminase levels plasma...
Background Extensive mononuclear cell infiltration is strongly correlated with liver damage in patients chronic hepatitis B virus (CHB) infection. Macrophages and infiltrating monocytes also participate the development of fibrosis animal models. However, little known regarding immunopathogenic role peripheral blood intrahepatic macrophages. Methodology/Principal Findings The frequencies, phenotypes, functions monocyte/macrophage subsets were analyzed 110 HBeAg positive CHB patients,...
A Chinese medical team managed Ebola virus disease (EVD) patients in Sierra Leone from October 2014 to March 2015 and attended 693 suspected patients, of whom 288 had confirmed disease.A retrospective study was conducted the with disease. Clinical symptoms, manifestations, serum viral load were analyzed compared among different groups for mortality survival time.Among EVD (149 male 139 female, median age 28 years, log 6.68), 98 died, 36 recovered, 154 lost follow-up. Common symptoms fever...
To obtain a comprehensive scenario of T cell profiles and synergistic immune responses, we performed single-cell RNA sequencing (scRNA-seq) on the peripheral cells 14 individuals with chronic human immunodeficiency virus 1 (HIV-1) infection, including nine treatment-naive (TP) eight antiretroviral therapy (ART) participants (of whom three were paired TP cases), compared results four healthy donors (HD). Through analyzing transcriptional CD4+ CD8+ cells, coupled assembled receptor sequences,...
Mucosal-associated invariant T (MAIT) cells are systemically depleted in human immunodeficiency virus type 1 (HIV-1) infected patients and not replenished even after successful combined antiretroviral therapy (cART). This study aimed to identify the mechanism underlying MAIT cell depletion.In present study, we applied flow cytometry, single-cell RNA sequencing immunohistochemical staining evaluate characteristics of pyroptotic a total 127 HIV-1 individuals, including 69 treatment-naive...
Persistent HIV infection results in a decrease absolute counts of CD4(+) CD25(+) regulatory T cells (Treg). To investigate the role decreased Treg regulation excessive activation and apoptosis CD8(+) human immunodeficiency virus (HIV)-1 infection, we characterized 83 HIV-1-infected individuals, including 19 long-term non-progressors (LTNPs) 51 typical progressors (TPs) who were treatment-naïve, 13 AIDS patients on highly active antiretroviral therapy (HAART), whom nine complete responders...
Background Treatment with nucleotide analogs is known to be effective in inhibiting HBV replication; however, patients chronic hepatitis B (CHB) often show a wide range of clinical responses these drugs. Therefore, the identification an early immunologic marker associated outcomes such cases critical for improved management. In our study, we aimed investigate whether viral load CHB affected ratio number regulatory T cells (Tregs) interleukin-17-producing helper (Th17) cells. Further,...
Background Mucosal-associated invariant T (MAIT) cells are considered to participate of the host immune response against acute severe respiratory syndrome coronavirus 2 (SARS-CoV-2) infection; however, single-cell transcriptomic profiling MAIT in patients with COVID-19 remains unexplored. Methods We performed RNA sequencing analyses on peripheral from 13 and 5 healthy donors. The transcriptional profiles cells, together assembled T-cell receptor sequences, were analyzed. Flow cytometry...
B cell follicles are immune-privileged sites where intensive HIV-1 replication and latency occur, preventing a permanent cure. Recent study showed that CXCR5+ NK cells in can inhibit SIV African green monkeys, but this has not been reported infected patients.Lymphocytes tissue sections of lymph node were collected from 11 positive antiretroviral therapy (ART)-naive 19 negative donors. We performed immunofluorescence RNA-scope to detect the location its relationship with RNA, flow cytometry...
Chronic inflammation and T cell dysregulation persist in individuals infected with human immunodeficiency virus type 1 (HIV-1), even after successful antiretroviral treatment. The mechanism involved is not fully understood. Here, we used Olink proteomics to comprehensively analyze the aberrant inflammation-related proteins (IRPs) chronic HIV-1-infected individuals, including 24 treatment-naïve 33 immunological responders, 38 non-responders. dysfunction was evaluated as exhaustion,...