A5046975418- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- Extracellular vesicles in disease
- Mechanical Circulatory Support Devices
- SARS-CoV-2 and COVID-19 Research
- 3D Printing in Biomedical Research
- Immune Cell Function and Interaction
- Systemic Sclerosis and Related Diseases
- RNA Interference and Gene Delivery
- Innovative Microfluidic and Catalytic Techniques Innovation
- Congenital heart defects research
- Advanced Biosensing Techniques and Applications
- Autoimmune Bullous Skin Diseases
- Respiratory viral infections research
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Cardiovascular Disease and Adiposity
- Inhalation and Respiratory Drug Delivery
- Receptor Mechanisms and Signaling
- Systemic Lupus Erythematosus Research
- Orthopedic Infections and Treatments
University Health Network
2021-2025
University of Toronto
2021-2025
Toronto General Hospital
2023-2025
Toronto General Hospital Research Institute
2024
Ionizable lipid nanoparticles (LNPs) pivotal to the success of COVID-19 mRNA (messenger RNA) vaccines hold substantial promise for expanding landscape mRNA-based therapies. Nevertheless, risk delivery off-target tissues highlights necessity LNPs with enhanced tissue selectivity. The intricate nature biological systems and inadequate knowledge structure–activity relationships emphasize significance high-throughput methods produce chemically diverse libraries screening. Here, we introduce a...
Despite tremendous progress in the development of mature heart-on-a-chip models, human cell-based models myocardial inflammation are lacking. Here, we bioengineered a vascularized with circulating immune cells to model severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced myocarditis. We observed hallmarks disease (COVID-19)-induced inflammation, as presence augmented secretion proinflammatory cytokines, triggered progressive impairment contractile function, and altered...
Abstract The fabrication of complex and stable vasculature in engineered cardiac tissues represents a significant hurdle towards building physiologically relevant models the heart. Here, we implemented 3D model vasculogenesis, incorporating endothelial cells (EC), stromal cells, human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CM) fibrin hydrogel. presence CMs disrupted vessel formation tissues, resulting upregulation activation markers altered extracellular vesicle (EV)...
Survival after lung transplantation (LTx) is hampered by uncontrolled inflammation and alloimmunity. Regulatory T-cells (Tregs) are being studied as a cellular therapy in solid organ transplantation. Whether these systemically administered Tregs can function at the appropriate location time an important concern. We hypothesised that vitro-expanded recipient-derived be delivered to donor lungs prior LTx via ex vivo perfusion (EVLP), maintaining their immunomodulatory ability.In rat model,...
Cardiovascular disease continues to take more human lives than all cancer combined, prompting the need for improved research models and treatment options. Despite a significant progress in development of mature heart-on-a-chip fibrosis cardiomyopathies starting from induced pluripotent stem cells (iPSCs), cell-based myocardial inflammation are lacking. Here, we bioengineered vascularized system with circulating immune model SARS-CoV-2-induced acute myocarditis. Briefly, observed hallmarks...
Abstract Survival after lung transplantation (LTx) is hampered by uncontrolled inflammation and alloimmunity. Regulatory T cells (Tregs) are being studied for post-implantation cell therapy in solid organ transplantation. Whether these systemically administered Tregs can function at the appropriate location time an important concern. We hypothesized that vitro expanded, recipient-derived be delivered to donor lungs prior LTx via ex vivo perfusion (EVLP), maintaining their immunomodulatory...