A5029657426- Estrogen and related hormone effects
- Cancer Risks and Factors
- Advanced Breast Cancer Therapies
- Breast Cancer Treatment Studies
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Global Cancer Incidence and Screening
- Molecular Biology Techniques and Applications
- Breast Lesions and Carcinomas
- Cancer Cells and Metastasis
- TGF-β signaling in diseases
- Prostate Cancer Treatment and Research
- Health, Environment, Cognitive Aging
- Nutrition, Genetics, and Disease
- Radiopharmaceutical Chemistry and Applications
- Phytoestrogen effects and research
- HER2/EGFR in Cancer Research
- MicroRNA in disease regulation
- Breast Implant and Reconstruction
- Cancer, Lipids, and Metabolism
- Nitric Oxide and Endothelin Effects
- Sarcoma Diagnosis and Treatment
- Skin Protection and Aging
- Photodynamic Therapy Research Studies
- Alcohol Consumption and Health Effects
Northwestern University
2006-2022
Huntsman Cancer Institute
2018-2020
University of Utah
2018-2020
University of Colorado Anschutz Medical Campus
2012-2016
American Cancer Society
2013
University of Illinois Chicago
2012-2013
University of Hawaiʻi at Mānoa
2012
University of Hawaii System
2012
Midwestern University
2012
Triple-negative breast cancer (TNBC) has the lowest 5-year survival rate of invasive carcinomas, and currently there are no approved targeted therapies for this aggressive form disease. The androgen receptor (AR) is expressed in up to one third TNBC we find that all AR(+) primary tumors tested display nuclear localization AR, indicative transcriptionally active receptors. While AR most abundant "luminal (LAR)" molecular subtype TNBC, here, first time, use both new-generation anti-androgen...
Abstract Soy isoflavone consumption may protect against breast cancer development. We conducted a phase IIB trial of soy supplementation to examine its effect on epithelial proliferation and other biomarkers in the healthy high-risk breast. One hundred twenty-six consented women underwent random fine-needle aspiration (rFNA); those with 4,000 or more cells were randomized double-blind 6-month intervention mixed isoflavones (PTIG-2535) placebo, followed by repeat rFNA. Cells examined for...
The mammary gland consists of cells with gene expression patterns reflecting their cellular origins, function, and spatiotemporal context. However, knowledge developmental kinetics mechanisms lineage specification is lacking. We address this significant gap by generating a single-cell transcriptome atlas encompassing embryonic, postnatal, adult mouse development. From these data, we map the chronology transcriptionally epigenetically distinct cell states distinguish fetal stem (fMaSCs) from...
Background: Alcohol consumption impairs type 1 cell‐mediated adaptive immune responses both in vivo and vitro. The present study investigated the effect of alcohol on antigen‐presenting cell (APC) populations cytokine production. Methods: BALB/c were fed ethanol‐containing, pair‐fed isocaloric liquid control, or solid diets for 11 days. Macrophage dendritic (DC) isolated by paramagenetic bead separation used to ovalbumin (OVA) highly purified syngeneic CD4 + T cells derived from DO11.10...
Profiling of RNA from mouse mammary epithelial cells (MECs) isolated on pregnancy day (P)14 and lactation (L)2 revealed that the majority differentially expressed microRNA declined precipitously between late lactation. The decline in miR-150, which exhibited greatest fold-decrease, was verified quantitatively qualitatively. To test hypothesis miR-150 is crucial for lactation, MEC-specific constitutive achieved by crossing ROSA26-lox-STOP-lox-miR-150 mice with WAP-driven Cre recombinase mice....
Previous studies have shown that progesterone concentrations in serum and nipple aspirate fluid (NAF) are significantly correlated premenopausal women, but estradiol not. We therefore sought to ascertain the patterns of both steroids NAF throughout menstrual cycle postmenopausal women. Simultaneous samples blood were obtained from 40 16 Premenopausal backdated following period. Steroids purified by high-performance liquid chromatography before quantification immunoassays. Serum followed...
Abstract Background CRIPTO is a multi-functional signaling protein that promotes stemness and oncogenesis. We previously developed antagonist, ALK4 L75A -Fc, showed it causes loss of the stem cell phenotype in normal mammary epithelia suggesting may similarly inhibit CRIPTO-dependent plasticity breast cancer cells. Methods focused on two triple negative lines (MDA-MB-231 MDA-MB-468) to measure effects -Fc behavior under nutrient deprivation endoplasmic reticulum stress. characterized...
Nipple aspiration fluid (NAF) use as a biosample is limited by the variable yield across studies. We investigated endocrine determinants of in an ongoing breast cancer case-control study.One-hundred and eighteen women yielding ≥2 μL NAF 120 non-yielders were included; serum hormones measured; differences median assessed using Wilcoxon rank-sum test. ORs 95% confidence intervals (95% CI) for yielder status relative to hormone levels estimated logistic regression, adjusting parity lactation,...
Sex steroid hormones contribute to breast cancer development, but data on concentrations of these within tissue are limited. We performed simultaneous multiparameter measurement sex steroids, epithelial cytology, and DNA methylation in 119 healthy women (54 pre- 65 postmenopausal) without a history cancer. Random fine-needle aspiration (rFNA) the was simultaneously with blood collection. Breast samples were analyzed by LC/MS-MS for estrone, estradiol, progesterone, androstenedione,...
Abstract Adult granulosa cell tumor (AGCT) is a subtype of sex-cord stromal tumors and accounts for ~5% all ovarian neoplasms. Nearly 100% AGCT cases are caused by an oncogenic point mutation in the Forkhead Box L2 (FOXL2) transcription factor. Weis-Banke et al. found this gain-of-function (FOXL2C134W) allows FOXL2 to hijack nuclear SMAD2/3/4 complex, downstream effector transforming growth factor- ß (TGF-ß), redirect novel sites, inducing epithelial mesenchymal transition (EMT) other...
Abstract Background: During mammary gland development, massive coordinated changes in protein expression govern the progression through pregnancy to lactation and involution. These dramatic are likely regulated part at translational level by microRNAs (miRNAs). We profiled miRNA epithelial cells (MECs) isolated from mice day 14 (P14) or 2 (L2) found that miR-150 is most significantly downregulated between lactation. Interestingly, was recently discovered be decreased mouse tumors compared...
<p>Supplementary Figure S1-Full length western blots and enzalutamide treatment of SUM159PT-AR<sup>F876L</sup> Supplementary S2-Xenograft caliper measurements total flux at randomization. S3-Xenograft tumor weights. S4-HCC1806 xenografts treated with enzalutamide. S5- Changes in proliferation apoptosis during scratch wound assay. S6-Signaling, proliferation, invasion SUM159PT shAR15 cells amphiregulin.</p>
<p>Supplemental Figure Legends</p>
<p>Supplementary Figure S1-Full length western blots and enzalutamide treatment of SUM159PT-AR<sup>F876L</sup> Supplementary S2-Xenograft caliper measurements total flux at randomization. S3-Xenograft tumor weights. S4-HCC1806 xenografts treated with enzalutamide. S5- Changes in proliferation apoptosis during scratch wound assay. S6-Signaling, proliferation, invasion SUM159PT shAR15 cells amphiregulin.</p>
<p>Supplemental Figure Legends</p>
<p>Table S1. Correlations between parameters that influence the quality of rFNA sample (119 women) Table S2. Correlation Breast and serum hormone concentrations. S3. Cytology according to menopausal status menstrual cycle.</p>
Abstract Transforming growth factor-beta (TGF-ß) is a master regulatory cytokine with pleotropic effects important in tumor development. TGF-ß1 binds TGF-ß receptor 2, which recruits and phosphorylates 1 (TGFBR1). TGFBR1 turn SMAD2 SMAD3. Phosphorylated SMAD2/3 (pSMAD2/3) complexes SMAD4 to translocate the nucleus enact transcriptional programs. In late-stage tumors, signaling oncogenic, promoting growth, invasion, immune suppression, tissue remodeling, epithelial mesenchymal transition...
<div>Abstract<p>Sex steroid hormones contribute to breast cancer development, but data on concentrations of these within tissue are limited. We performed simultaneous multiparameter measurement sex steroids, epithelial cytology, and DNA methylation in 119 healthy women (54 pre- 65 postmenopausal) without a history cancer. Random fine-needle aspiration (rFNA) the was simultaneously with blood collection. Breast samples were analyzed by LC/MS-MS for estrone, estradiol,...
Abstract Transforming growth factor-β (TGF-β) receptors regulate SMAD signal transduction and a wide-range of biological processes including, wound healing, angiogenesis, immune modulation, epithelial-mesenchymal transition, cell differentiation, apoptosis, growth, motility. In transformed cells, TGF-β signaling is frequently exploited to reshape the architecture tumor microenvironment (TME) establish fibrotic barrier that insulates from surrounding normal tissue. As result, immunological...
Abstract Ovarian cancer is still associated with poor prognosis and remains amongst the leading causes of related deaths in females. High recurrence rates, resistance to chemotherapy, meager outcome highlight need for improved therapies that stem from understanding complex multifactorial etiology ovarian malignancies. Transforming growth factor-β (TGF-β) signaling can regulate both oncogenesis metastasis (Kumri et al. 2021). This, part, done by affecting apoptosis survival mechanisms,...
Abstract Ewing sarcoma (EWS) is the second most common bone and soft tissue in children adolescents accounts for approximately 2% of all childhood cancer diagnoses. EWS a highly aggressive cancer: overall 5-year survival rate localized metastatic are 67 38%, respectively. Each year, 200 United States diagnosed with EWS. Genetically, nearly have chromosomal translocations which member FET gene family fused an ETS transcription factor, 85% cases being EWSR1-FLI1. Cyclin-dependent kinase 9...
<p>Table S1. Correlations between parameters that influence the quality of rFNA sample (119 women) Table S2. Correlation Breast and serum hormone concentrations. S3. Cytology according to menopausal status menstrual cycle.</p>