A5070970539- Cancer Cells and Metastasis
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Pancreatitis Pathology and Treatment
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- Diet, Metabolism, and Disease
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Cancer Mechanisms and Therapy
- Biochemical Analysis and Sensing Techniques
- Advanced Breast Cancer Therapies
- melanin and skin pigmentation
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Estrogen and related hormone effects
- Wnt/β-catenin signaling in development and cancer
- Animal Genetics and Reproduction
- FOXO transcription factor regulation
Salk Institute for Biological Studies
2018-2021
Cancer Research UK
2014-2015
University of Cambridge
2014-2015
Université Libre de Bruxelles
2015
Indian Institute of Science Bangalore
2009
The mammary gland consists of cells with gene expression patterns reflecting their cellular origins, function, and spatiotemporal context. However, knowledge developmental kinetics mechanisms lineage specification is lacking. We address this significant gap by generating a single-cell transcriptome atlas encompassing embryonic, postnatal, adult mouse development. From these data, we map the chronology transcriptionally epigenetically distinct cell states distinguish fetal stem (fMaSCs) from...
Technological improvements enable single-cell epigenetic analyses of organ development. We reasoned that high-resolution chromatin accessibility mapping would provide needed insight into the reprogramming and transcriptional regulators involved in normal mammary gland Here, we a resource for murine development from peak fetal stem cell (fMaSC) functional activity late embryogenesis to differentiation adult basal luminal cells. find landscape within individual cells predicts both gene...
Cancer stem cells exhibit close resemblance to normal in phenotype as well function. Hence, studying cell behavior is important understanding cancer pathogenesis. It has recently been shown that human breast can be enriched suspension cultures mammospheres. However, little known about the of these long-term cultures. Since extensive self-renewal potential hallmark cells, we undertook a detailed phenotypic and functional characterization mammospheres over passages.Single suspensions derived...
Abstract The cycling properties of mammary stem and progenitor cells is not well understood. To determine the division these cells, we administered synthetic nucleosides for varying periods time to mice at different stages postnatal development monitored rate uptake in cell compartments. Here show that most adult virgin gland restricted oestrogen receptor-expressing luminal lineage. Our data also demonstrate receptor-expressing, milk basal subpopulations have telomere lengths kinetics are...
Abstract Introduction Lineage tracing using inducible genetic labeling has emerged to be a powerful method for interrogating the developmental fate of cells in intact tissues. A common induction mechanism is use tamoxifen-dependent Cre recombinase (CreER and CreER T2 ), but effects tamoxifen at doses normally used lineage-tracing studies on normal adult mammary gland homeostasis are not known. Methods We flow cytometry immunostaining glands determine whether varying skew distribution...
Summary It has only recently become possible to obtain single-cell level resolution of the epigenetic changes that occur during organ development. We reasoned precision chromatin accessibility mapping mammary gland development could provide needed insight into reprogramming and transcriptional regulators involved in normal Here, we first resource for murine from peak fetal stem cell (fMaSC) functional activity late embryogenesis differentiation adult basal luminal cells. find landscape...
ABSTRACT BACKGROUND & AIMS Acinar to ductal metaplasia (ADM) occurs in the pancreas response tissue injury and is a potential precursor for adenocarcinoma. The goal of these studies was define populations arising from ADM, associated transcriptional changes, markers disease progression. METHODS cells were lineage-traced with enhanced yellow fluorescent protein (EYFP) follow their fate upon injury. Transcripts over 13,000 EYFP+ determined using single-cell RNA sequencing (scRNA-seq)....
Abstract Despite numerous advances in our understanding of pancreatic ductal adenocarcinoma (PDA) genetics and biology, this disease is expected to become the second leading cause cancer-related U.S. deaths within next few years. Incomplete how it arises precludes development early detection interception strategies improve therapeutic outcomes. Acinar metaplasia involving genesis tuft cells one step PDA formation, but their functional significance has remained obscure due rarity a lack...