A5053576640- Milk Quality and Mastitis in Dairy Cows
- Immune Response and Inflammation
- Animal Virus Infections Studies
- Reproductive Physiology in Livestock
- Cell Adhesion Molecules Research
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Reproductive System and Pregnancy
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Animal health and immunology
- Respiratory viral infections research
- Influenza Virus Research Studies
- Viral Infections and Immunology Research
- Prion Diseases and Protein Misfolding
- Genetic and phenotypic traits in livestock
- Monoclonal and Polyclonal Antibodies Research
- Infant Nutrition and Health
- T-cell and Retrovirus Studies
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Neurological diseases and metabolism
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Effects of Environmental Stressors on Livestock
National Animal Disease Center
2010-2024
Agricultural Research Service
2009-2024
United States Department of Agriculture
1993-2018
The University of Texas MD Anderson Cancer Center
2011
Kansas State University
2011
Iowa State University
1992-2008
Johns Hopkins University
2002
Massachusetts General Hospital
2002
Harvard University
2002
Johns Hopkins Hospital
2002
SUMMARY Neutrophils from 8 Holstein heifers were evaluated for function during the periparturient period. Random migration ingestion of bacteria, superoxide anion production, native (nonenhanced) chemiluminescence, iodination, and antibody-dependent, cell-mediated cytotoxicity by neutrophils determined. Foremilk samples bacteria. Significant ( P < 0.05) increases in random neutrophils, chemiluminescence evident 2 weeks before parturition then decreased dramatically first week after...
Two point mutations were identified within the gene encoding bovine CD18 in a Holstein calf afflicted with leukocyte adhesion deficiency (LAD). One mutation causes an aspartic acid to glycine substitution at amino 128 (D128G) highly conserved extracellular region of this glycoprotein, where several have been found cause human LAD. The other is silent. Twenty calves clinical symptoms LAD tested, and all homozygous for D128G allele. In addition, two allele during widespread DNA testing, both...
Abstract The responsiveness of bovine neutrophil L-selectin and CD18 to in vivo glucocorticoid administration was characterized by flow cytometric analysis. Blood sampled intensively from dairy cows treated for 3 days with placebo, cortisol, or dexamethasone. Immunostaining performed on whole blood (100 μ1) that left unstimulated stimulated platelet-activating factor (PAF; 1 μg/ml blood) prior incubation fluorescein isothiocyanate-conjugated monoclonal antibodies against CD18. Results were...
SUMMARY Lymphocytes from 8 Holstein heifers were evaluated during the periparturient period for mitogen-induced lymphocyte blastogenic responses. Phytohemagglutinin P- and concanavalin A-induced blastogenesis increased 2 3 weeks before parturition, respectively. However, by first week after was markedly impaired.
SUMMARY Leukocytosis (34,600 wbc /μl of blood) was detected in an apparently healthy 7-day-old Holstein heifer. Analysis blood samples obtained over the next 41 days revealed chronic progressive neutrophilia, which peaked at > 85% neutrophils and exceeded 100,000 /μl. In vitro assessment isolated from heifer 38 45 age selected functional abnormalities. Endocytosis immunoglobulin-opsonized Staphylococcus aureus killing this test organism by calf’s were significantly diminished, as...
Generation of inflammatory mediators and leukocyte recruitment to infection at an epithelial surface were studied during Escherichia coli-induced mastitis. One uninfected gland each eight midlactation cows was challenged with only 30 CFU E. coli McDonald strain 487, a serum-resistant isolate from cow Bacteria grew logarithmically the first 10 12 h after challenge, reaching concentrations more than 10(5) CFU/ml no detectable host response this time. An intense reaction began approximately...
The objective of this report was to characterize the enhanced clinical disease and lung lesions observed in pigs vaccinated with inactivated H1N2 swine δ-cluster influenza A virus challenged pandemic 2009 A/H1N1 human virus. Eighty-four, 6-week-old, cross-bred were randomly allocated into 3 groups 28 represent vaccinated/challenged (V/C), non-vaccinated/challenged (NV/C), non-vaccinated/non-challenged (NV/NC) control groups. Pigs intratracheally inoculated pH1N1and euthanized at 1, 2, 5, 21...
It is generally accepted that the beta 2-integrin restricted to mononuclear leukocytes. The objective of this study was determine whether neutrophils can also express 1-integrin (specifically alpha 4 1) and support neutrophil adhesion endothelial cells extracellular matrix. We stimulated with dihydrocytochalasin B (DHCB) various chemotactic stimuli observed induced via (CD18), whereas DHCB either fMLP, PAF, or IL-8 endothelium protein-coated plastic not inhibitable by anti-CD18 antibody....
Abstract Objective To define causes of increased susceptibility to coliform mastitis after parturition. Animals 12 healthy Holstein cows assigned 2 groups. Group-1 (n = 6) had calved between 6 and 10 days earlier. Group-2 were in midlactation. Procedure Cows from each group paired challenge exposed with Escherichia coli 1 mammary gland. Mastitis severity was determined by bacterial concentration milk, pyrexia, milk production. Measures host defense neutrophil chemotaxis, adhesion molecule...
SUMMARY The role of interleukin-1 ( il -1), interleukin-6 - 6), and tumor necrosis factor α during endotoxin-induced mastitis in cows was characterized. Six had 10 μg Escherichia coli lipopolysaccharide infused into 1 mammary gland. Three other served as nontreated controls. Within 1.5 to 2.5 hours after infusion, endotoxin caused obvious edema the gland increased serum albumin concentration milk glands 6 times. Milk somatic cell count began increase 3 5 infusion all treated glands. At 7...
Fumonisins are myocotoxins produced by Fusarium moniliforme and F. proliferatum, common molds of corn in North America. The toxin is at especially high concentrations screenings. toxic to swine horses, but effects these toxins cattle have not been evaluated. This experiment was conducted determine the feeding fumonisins levels known be horses. A total 18 crossbred feeder calves were fed diets containing 15, 31, or 148 µg/g for 31 d. Feed consumption, weight gain, complete blood count, serum...
ABSTRACT Vaccines provide a primary means to limit disease but may not be effective at blocking infection and pathogen transmission. The objective of the present study was evaluate efficacy commercial inactivated swine influenza A virus (IAV) vaccines experimental live attenuated (LAIV) against with H3N2 subsequent indirect transmission naive pigs. evaluated similar H3N2v detected in humans during 2011-2012, which associated contact agricultural fairs. One vaccine provided partial protection...