A5050943214- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Renal and related cancers
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Renal cell carcinoma treatment
- MicroRNA in disease regulation
- Bladder and Urothelial Cancer Treatments
- RNA modifications and cancer
- Genetic and Kidney Cyst Diseases
- Extracellular vesicles in disease
- RNA Research and Splicing
- Cancer Cells and Metastasis
- Folate and B Vitamins Research
- Neonatal Respiratory Health Research
- Glycosylation and Glycoproteins Research
- Cancer Genomics and Diagnostics
- Polyamine Metabolism and Applications
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- HIV-related health complications and treatments
- Wnt/β-catenin signaling in development and cancer
- Tissue Engineering and Regenerative Medicine
- Nuclear Structure and Function
- Seaweed-derived Bioactive Compounds
China Medical University
2010-2020
Harvard University
2014
The present experiment was designed for exploring the regulatory mechanism of circ‐CEP128/miR‐145‐5p/ MYD88 axis in bladder cancer. MiRNAs and circRNAs expression data were derived from Gene Expression Omnibus database with tumor tissues paracarcinoma tissue samples. Differentially expressed genes analyzed via R software. Interaction network differently miRNAs mRNA established by means Cytoscape CircCEP128 miR‐145‐5p levels determined using qRT‐PCR. MAPK signaling‐related proteins , p38, ERK...
Abstract Background The study was aimed to investigate the influence of circ_0058063 on tumorigenesis as well regulatory mechanism circ_0058063/miR‐145‐5p/ CDK6 pathway in bladder cancer (BC). Methods Bioinformatics analysis used screen highly expressed circle RNA (circRNA) and search its downstream microRNA (miRNA) protein. expression level circRNA, miRNA, BC cell lines T24 J82 were determined by quantitative real‐time polymerase chain reaction (qRT‐PCR) western blot. Small interfering...
Background: Lentinan (LNT), an isolated traditional Chinese herbal component, has antitumor potential. In the current study, intrinsic mechanism of LNT-induced immunity against bladder cancer was explored in a mouse model. Methods: model cancer, we used flow cytometry to detect LNT caused population changes T cells, macrophages, MDSC and Treg cells. ELISA evaluate cytokines expression supernatant splenocytes. Results: We found that administration increased proportions CD3+CD4+ CD3+CD8+ cell...
The aims of this study were to determine the methylation and expression status secreted Frizzled-related protein 1 (SFRP1) in bladder cancer, explore mechanisms involved role SFRP1 pathogenesis cancer. mRNA was detected by reverse transcription PCR (RT-PCR). DNA determined methylation-specific using western blotting. results present demonstrated that methylated cancer cell lines T24 5637, but not SCaBER cells. After treating 5637 cells with a demethylating agent, expressed protein. Among 45...
To better understand the contribution of dysregulated DNA methyltransferase 1 (DNMT1) expression to progression and biology clear cell renal carcinoma (ccRCC).We examined differences in DNMT1 89 ccRCC 22 normal tissue samples by immunohistochemistry. In addition, changes viability, apoptosis, colony formation invading ability lines (786-0 Caki-1) were assessed after transfection with siRNA.We found protein was significantly higher expressed than that no-tumor tissues (56.2% 27.3%,...
Supplemental oxygen therapy (hyperoxia) is a widely used treatment for alveolar hypoxia in preterm infants. Despite being closely monitored, hyperoxia exposure believed to undermine neonatal nephrogenesis and renal function caused by elevated oxidative stress. Previous studies have mostly focused on the hyperoxia-induced impairment of glomerular development, while long-term impact tubular development regulatory component involved this process remain be clarified. Here, we examined histology...
Bladder cancer (BC) is one of the tumors which occur most frequently in urological system, but less known about expression tight junction proteins and its clinical significance BC. In this study, claudin-4, zonula occludens-1 (ZO-1) nucleic acid-binding protein (ZONAB), BC tissues, adjacent nontumor tissue (ANTT), cell lines was examined by Western blotting, semiquantitative RT-PCR, immunohistochemistry, then, these investigated. The mRNA ZONAB were significantly upregulated, while those...
Abstract Background Bladder cancer (BC) is the most common malignancy of urinary system and cisplatin (DDP) remains only chemotherapy option for treatment BC at advanced stage. The critical molecules involved in regulation resistance are still largely unknown. CircRNAs has been demonstrated to be tumorigenesis development drug various cells. CircCEP128 contributed progression by regulating miR-145-5p/MYD88/MAKP axis. However, functions molecular mechanisms circCEP128 DDP bladder cells remain...
Objective To investigate the expression of Snail in bladder urothelial carcinoma and evaluate its relationship with E-cadherin a subset T cell groups.Methods Immunohistochemical method was used to detect proteins tissue from 156 cases 80 para-cancerous mucosa. The status analyzed clinico-pathological data. Meanwhile, correlations CD4+, CD8+, CD4+/CD8+ were analyzed.Results positive rate 65.4％ , which significantly higher than that mucosa (48.8％). correlated clinical stage, pathological...
Objective To measure the expression of SFRP1 in bladder cancer and explore corresponding mechanism,in order to study roles pathogenesis cancer.Methods mRNA was detected by RT-PCR.Methylation status methylation specific PCR.SFRP1 protein determined Western blotting.Results methylated cell lines T24 5637,not SCaBER.SFRP1 were SCaBER,but not 5637.Six hours after treating 5637 with 5'-aza-deoxycytidine,both expressed protein.In 45 cancers,methylation 28 (62.2％).In matched adjacent tissues,6...