Aims: Our main interest is to check if programmed cell death (PCD) can occur in prokaryotic algae and the morphological biochemical features of PCD are conserved. Methods Results: Using TUNEL labelling, fluorescence light microscopy DNA gel electrophoresis, we found that with similar those metazoan could be induced different Anabaena strains after exposure univalent‐cation salts at moderate concentration. These included specific fragmentation, cytoplasmic vacuolation, progressive...

Transitional cell carcinoma (TCC) is one of the most common types malignancies and a leading cause genitourinary system cancer mortality worldwide. The tumor suppressor gene FOXO1, member forkhead box O (FOXO) subfamily transcription factors, downregulated in number cancers, including TCC; however, underlying mechanisms are poorly understood. In present study, we used microRNA (miRNA) target prediction algorithms to identify conserved potential miR-96 binding site 3'-untranslated region...

Bladder cancer (BC) is one of the most common cancers world-wide with a poor prognosis. Non-SMC (Structural Maintenance Chromosomes)-condensin I complex subunit H (NCAPH) regulatory condensin and plays an important role in tumorigenesis progression several types cancers. However, NCAPH BC remains unknown. In this study, we tried to reveal biological functions BC. We detected expressions adjacent tissues, cells lines. Subsequently, gain- loss-of-function experiments were performed determine...

It is known that secreted protein acidic and cysteine rich (osteonectin), cwcv kazal-like domains proteoglycan 2 (SPOCK2) plays a significant role in the development progression of several human cancers; however, SPOCK2 prostate cancer (PCa) remains unclear. This study aimed to find mechanism PCa.The messenger ribonucleic acid (mRNA) expression PCa tissue was detected by real-time polymerase chain reaction (PCR). Upregulation gene achieved using DU145 LNCaP cells transfecting with...

Aim: Prostate cancer (PCa) is the most common malignancy found in males worldwide. Although it mostly indolent, PCa still poses a serious threat to long-term health. Materials & methods: The Cancer Genome Atlas data were randomly divided into training and validation groups. Least absolute shrinkage selection operator regression on DNA methylation group was conducted build model, which validated group. Weighted correlation network analysis RNA-seq identify therapy target. Functional (western...

The presence of arachidonate 12-lipoxygenase (12-LOX) potentiates prostate cancer (PCa) progression and therefore may be a good therapeutic target and/or potential diagnostic predictor for PCa. In this study, we examined the expression 12-LOX in PCa stem cells (PCa SCs) to test if it can serve as unique marker SCs. To end, isolated cell-like side population (SP) from human cell line DU-145 by flow cytometry-based SP technique. comprised small cells. had an up-regulation ATP-binding cassette...

The aberrant expression of Eya2 has been observed in a wide range cancer types. However, the clinical significance and biological effects EYA2 human prostate remain unknown. In this study, we showed that increased levels protein correlated with advanced TNM stage, T higher Gleason score. Data from Cancer Genome Atlas (TCGA) cohort consistently revealed mRNA was positively score, positive nodal metastasis cancer. Furthermore, data Oncomine database tissues compared normal tissues. also cell...

BACKGROUND We sought to investigate the expression of KPNA2 in bladder cancer (BC) and its relationship with prognosis, analyze potential mechanism promoting BC progression. MATERIAL AND METHODS The RNA-seq data on from Cancer Genome Atlas (TCGA) database were imported into R statistical software for differential analysis. clinical patients screened analyzed software. survival curve was drawn Kaplan-Meier Plotter. 4 human cell lines a epithelial line detected by quantitative real-time...

PF4V1 is a novel protein in inflammation, angiogenesis, and cancer. However, the pathogenesis, underlying mechanisms, prognostic value of prostate cancer (PCa) are still unclear.The expression relation with survival were analyzed based on large sample size Cancer Genome Atlas. In vitro, overexpression was conducted DU145 LNCaP cells. Cell Counting Kit-8, colony formation, wound healing, Transwell® assays preformed to test biological functions miR-875-3p PCa. Western blotting used measure...

An integrated analysis of key microRNAs, regulatory pathways and clinical relevance in bladder cancer Dongyang Li,1 Xuanyu Hao,2 Yongsheng Song1 1Department Urology, Shengjing Hospital China Medical University, Shenyang, Liaoning 110004, People’s Republic China; 2Department Rheumatology Immunology, 110022, Objective: The aim this study was to identify the microRNAs (miRNAs) their networks (BC). Materials methods: Three miRNA three gene expression microarray datasets were downloaded for...

The clinical efficacy and mechanism of Pralatrexate (PTX) combined with Palbociclib Isethionate (PAL) in the treatment bladder cancer patients was investigated. A retrospective analysis medical records 82 admitted to Shengjing Hospital China Medical University from February 2015 2018 performed. Patients treated PTX PAL served as study group (42 cases) conventional GC (gemcitabine plus cisplatin) chemotherapy regimen were control (40 cases). Changes liver function indexes before after...

Our previous study found that plate factor-4 variant (CXCL4L1) was downregulated in the serum of patients with prostate cancer (PCa). The aim present to investigate prognostic value CXCL4L1 PCa. In total, 213 PCa treated radical prostatectomy were enrolled and peripheral blood samples all collected. Expression different tumor stages grades measured by enzyme-linked immunosorbent assay (ELISA). Kaplan–Meier method applied estimate progression castration-resistant (CRPC), metastasis,...

Abstract δ‐Catenin is a unique member of the catenin family and proved to be overexpressed in diverse human cancer types. However, clinical significance underling mechanism δ‐catenin expression renal cell carcinoma (RCC) remain elusive. Herein, we detected protein 28 specimens paired tissues normal by Western blot analysis. 58 cases was also examined immunohistochemistry, its association with clinicopathological factors analyzed statistical In vitro vivo assays were employed further explore...

Background: The relationship between microcirculatory disorders and testicular spermatogenesis is an area of ongoing interest among urologists. objective this prospective observational study was to investigate the correlation microcirculation spermatogenesis, as well predictive value ultrasonic microvascular density (UMVD) ultrasonographic volume estimation (UVE) in successful sperm retrieval men with non-obstructive azoospermia (NOA). Methods: Testicular UMVD derived from Angio PLUSTM...

Objective To investigate the expression of Snail in bladder urothelial carcinoma and evaluate its relationship with E-cadherin a subset T cell groups.Methods Immunohistochemical method was used to detect proteins tissue from 156 cases 80 para-cancerous mucosa. The status analyzed clinico-pathological data. Meanwhile, correlations CD4+, CD8+, CD4+/CD8+ were analyzed.Results positive rate 65.4％ , which significantly higher than that mucosa (48.8％). correlated clinical stage, pathological...