A5076562856- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Mycobacterium research and diagnosis
- Bacterial biofilms and quorum sensing
- Streptococcal Infections and Treatments
- Pharmaceutical and Antibiotic Environmental Impacts
- Infections and bacterial resistance
- Enterobacteriaceae and Cronobacter Research
- Biosensors and Analytical Detection
- Anesthesia and Neurotoxicity Research
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Antibiotic Use and Resistance
- Plant Pathogenic Bacteria Studies
- Infective Endocarditis Diagnosis and Management
- Infection Control in Healthcare
- Anesthesia and Sedative Agents
- Cardiac, Anesthesia and Surgical Outcomes
- Neurosurgical Procedures and Complications
- Glycogen Storage Diseases and Myoclonus
- Mitochondrial Function and Pathology
Universidade de São Paulo
2013-2024
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2017-2024
Instituto de Medicina Tropical
2021-2022
Universidade Federal de São Carlos
2022
Instituto de Medicina Avançada
2021
In vitro and clinical studies using parenteral fosfomycin have suggested the possibility of this drug against infections caused by MDR microorganisms. The aim study was to describe a case series patients treated with who had severe pan-drug-resistant Gram-negative bacteria.We prospective cases hospitalized bacteria resistant β-lactams colistin, 16 g daily for 10-14 days. Isolates were tested antimicrobial susceptibility synergism meropenem. We resistance genes performed typing PCR...
Health care associated infections (HAIs) are currently among the major challenges to of hematopoietic stem cell transplantation (HSCT) patients. The objective present study was evaluate impact 2% chlorhexidine (CHG) bathing on incidence colonization and infection with vancomycin-resistant Enterococcus (VRE), multidrug-resistant (MDR) gram-negative pathogens, their CHG minimum inhibitory concentration (MIC) after intervention. A quasi-experimental duration 9 years conducted. VRE infection,...
Abstract Carbapenem-resistant Enterobacteriaceae are a worldwide health problem and isolates carrying both bla KPC-2 NDM-1 unusual. Here we describe the microbiological clinical characteristics of five cases bloodstream infections (BSI) caused by carbapenem-resistant Klebsiella pneumoniae Serratia marcescens having . Of blood samples, three from hematopoietic stem cell transplantation patients, one renal transplant patient, surgical patient. All patients lived in low-income neighbourhoods...
Enterobacterales and other non-fermenting Gram-negative bacteria have become a threat worldwide owing to the frequency of multidrug resistance in these pathogens. On hand, efficacious therapeutic options are quickly diminishing. The aims this study were describe susceptibility 50 multiresistant bacteria, mostly pan-resistant, against old less-used antimicrobial drugs investigate presence genes. A total genetically distinct isolates included study, including 14 Acinetobacter baumannii...
Carbapenems resistance in Enterobacter spp. has increased the last decade, few studies, however, described mechanisms of this bacterium. This study evaluated clonality and carbapenems clinical isolates identified three hospitals Brazil (Hospital A, B C) over 7-year.Antibiotics sensitivity, pulsed-field gel electrophoresis (PFGE), PCR for carbapenemase efflux pump genes were performed all carbapenems-resistant isolates. Outer-membrane protein (OMP) was based on PFGE profile.A total 130 spp...
Stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in the last decade. Increased resistance to sulfamethoxazole/trimethoprim (SMX/TMP) been reported S. strains past few years, leading therapeutic options. We conducted a prospective multicenter study at two Brazilian teaching hospitals that identified isolates and evaluated their antimicrobial susceptibility profile, SMX/TMP genes clonality profile. A total of 106 non-repeated clinical samples were evaluated....
The plasmid-mediated colistin-resistance gene named mcr-1 has been recently described in different countries and it became a public health challenge. Of note, few studies have addressed the spread of Escherichia coli harboring both, community hospital settings. A total seven colistin-resistant E. carrying mcr-1, collected from 2016 to 2018, (n=4), healthcare-acquired infections (n=2) colonization (n=1) were identified three high complexity hospitals Sao Paulo, Brazil. These isolates screened...
Carbapenem-resistant Klebsiella pneumoniae (CRK) infections are a growing concern in immunocompromised patients. The aim of the present study was to evaluate impact CRK colonization and infection overall mortality for haematopoietic stem-cell transplant (HSCT) We also aimed investigate resistance virulence profiles isolates assess their epidemiological genetic relatedness. Patients HSCT unit were screened with weekly rectal swab or stool cultures placed under contact precautions. defined as...
Despite the widespread use of chlorhexidine (CHX) to prevent infection, data regarding in vitro action CHX against methicillin-resistant Staphylococcus aureus (MRSA) are limited. Clinical isolates from Hospital das Clinicas, Sao Paulo, Brazil, identified during 2002/2003 and 2012/2013 were studied describe susceptibility mupirocin, molecular characteristics, virulence profile MRSA. Susceptibility test Mupirocin was performed by disk diffusion method agar dilution technique. PCR for genes,...
The spread of multidrug-resistant (MDR) Klebsiella pneumoniae, especially carbapenems and aminoglycosides, is a global public health challenge. Studying the genetic relatedness bacteria from various sources, such as humans, animals, food, environment, can help identify potential routes transmission extent dissemination. In this study, we examined phenotypes features MDR K. pneumoniae isolates. Seventy strains were selected different sources (clinics (n=55), environmental (n=7), animals...
This study describes an outbreak of Streptococcus equi subspecies zooepidemicus infections that caused meningoencephalitis and bacteremia related to unpasteurized milk consumption in northeastern Brazil. Epidemiological investigations a brief literature review were conducted. Strains with possible neurotropism had not been identified Brazil before these cases; however, 2023, another case by sp. was described, revealing the need maintain surveillance highlighting neurotropic strains continue...
Background: Assessing the risk of multidrug-resistant colonization and infections is pivotal for optimizing empirical therapy in hematopoietic stem cell transplants (HSCTs). Limited data exist on extended-spectrum β-lactamase-producing Enterobacterales (ESBL-E) this population. This study aimed to assess whether ESBL-E constitutes a factor bloodstream infection (BSI) evaluate HSCT recipients. Methods: A retrospective analysis BSI patients was conducted from August 2019 June 2022. Weekly...
Purpose: To evaluate in vitro efficacy of amikacin combined with meropenem, colistin, and ceftazidime/avibactam against diverse multidrug-resistant isolates K. pneumoniae (n=40), A. baumannii (n=20), S. marcescens (n=12), using three methods. Methods: Antimicrobial combinations were tested via Epsilometer strip crossing, disk approximation, time-kill assays. Results: Amikacin colistin showed synergy 70% (28/40) 75% (9/12) isolates. Meropenem limited activity 25% (5/20) Minor errors observed...
Aim: To assess chlorhexidine (CHG) susceptibility among resident microbiota from hands of healthcare workers (HCWs) after a period continuous CHG soap use for handwashing compared to plain soap. Methods: Before-after crossover study in not-for-profit private tertiary care hospital São Paulo, Brazil April 2016 2018. We alternated control (plain soap) and intervention periods (CHG selected wards at intervals four eight months. collected the HCWs hand sample before each swap along with...
ABSTRACT The COVID-19 pandemic has given rise to numerous commercially available antigen rapid diagnostic tests (Ag-RDTs). To generate and share accurate independent data with the global community, multi-site prospective evaluations of Ag-RDTs are required. This report describes clinical evaluation OnSite Rapid Test (CTK Biotech, California, USA) in Brazil United Kingdom. A total 496 paired nasopharyngeal (NP) swabs were collected from symptomatic healthcare workers at Hospital das Clínicas...
We described a MRSA bloodstream infection outbreak that was rapidly identified and controlled in Neonatal Intensive Care Unit after implementation of bundle measures, including PCR-screening HCW decolonization. found 35% healthcare workers(HCW) colonized with S. aureus by PCR, one them presented skin lesion positive for MSSA (same clone spa type than two patients). Our findings raise the hypothesis could be related to colonization.
Abstract Carbapenem-resistant Enterobacteriaceae are a worldwide health problem and isolates carrying both bla KPC-2 NDM-1 unusual. Here we describe the microbiological clinical characteristics of five cases bloodstream infections (BSI) caused by carbapenem-resistant Klebsiella pneumoniae Serratia marcescens having . Of blood samples, three from hematopoietic stem cell transplantation patients, one renal transplant patient, soft tissue surgical patient. All patients lived in low-income...