A5076144019- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Hematopoietic Stem Cell Transplantation
- TGF-β signaling in diseases
- Cancer Cells and Metastasis
- Transgenic Plants and Applications
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Diabetes and associated disorders
- Lipoproteins and Cardiovascular Health
- Cytomegalovirus and herpesvirus research
- Genetic factors in colorectal cancer
- Atherosclerosis and Cardiovascular Diseases
- Biosimilars and Bioanalytical Methods
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- T-cell and Retrovirus Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Monoclonal and Polyclonal Antibodies Research
- Bone Tissue Engineering Materials
- Xenotransplantation and immune response
- Cell Adhesion Molecules Research
British Columbia Children's Hospital
2019-2024
University of British Columbia
2013-2024
Canada's Michael Smith Genome Sciences Centre
2017-2022
McGill University
2015-2020
The University of Queensland
2008
QIMR Berghofer Medical Research Institute
2008
Local tissue mechanics play a critical role in cell function, but measuring these properties at cellular length scales living 3D tissues can present considerable challenges. Here we thermoresponsive, smart material microgels that be dispersed or injected into and optically assayed to measure residual elasticity after creep over several weeks. We first develop characterize the sensors, demonstrate internal mechanical profiles of live multicellular spheroids mapped high resolutions reveal...
Abstract Regulatory T cell (Treg) therapy is a potential curative approach for variety of immune-mediated conditions, including autoimmunity and transplantation, in which there pathological tissue damage. In mice, IL-33R (ST2)–expressing Tregs mediate repair by producing the growth factor amphiregulin, but whether similar tissue-reparative exist humans remains unclear. We show that human blood multiple types produced this was neither unique feature nor selectively upregulated tissues. Human...
Graft-versus-host disease (GvHD) is a significant complication of allogeneic hematopoietic stem cell transplantation. In order to develop new therapeutic approaches, there need recapitulate GvHD effects in pre-clinical, vivo systems, such as mouse and humanized models. models GvHD, mice are reconstituted with human immune cells, which become activated by xenogeneic (xeno) stimuli, causing multi-system disorder known xenoGvHD. Testing the ability therapies prevent or delay development...
Chronic graft-versus-host disease (cGvHD) is a major cause of morbidity after hematopoietic stem cell transplantation (HSCT). In large patient populations, we have shown CD56bright natural killer (NK) population to strongly associate with lack cGvHD and hypothesize that these cells function suppress cGvHD. We aimed isolate define the characteristics regulatory NK (NKreg) associated suppression Immunophenotypic evaluation pediatric found be perforin-, Granzyme B-, CD335+. Transcriptome...
Statin drugs are one of the most commonly prescribed pharmaceuticals by physicians. By blocking rate-limiting step in cholesterol biosynthesis pathway, statins inhibit synthesis, which benefits patient health. However, since many other important cellular processes regulated within this they may also be influenced statin therapy. These pleiotropic effects have not been fully investigated, but believed to positively influence endothelial cells (ECs), line vasculature a confluent monolayer. Few...
Abstract T regulatory cells (Tregs) are critical for self-tolerance and being pursued as a potential cell therapy in transplantation autoimmunity. While polyclonal Tregs can prevent transplant rejection mice, allo-antigen specific more potent. A chimeric antigen receptor (CAR) may be an effective way to make ag-specific suitable numbers the clinic. CARs created by combining antibody variable domains with signaling domains. However, Treg must a) function within distinct network of b)...
Abstract Local stiffness plays a critical role in cell function, but measuring rigidity at cellular length scales living 3D tissues presents considerable challenges. Here we present thermoresponsive, smart material microgels that can be dispersed or injected into and optically assayed to measure internal tissue over several weeks. We first develop the design principles across physiological ranges, with spatial resolutions approaching of individual cells. Using microfabricated sensors,...
Markey, K1,2; Burman, A1; Kuns, R1; Raffelt, N1; Morris, E1; Banovic, T1; Korner, H3; MacDonald, Hill, G1,2 Author Information
Introduction: Animal and early clinical studies have shown that regulatory T cell (Treg) therapy can prevent graft-versus-host disease (GVHD) following hematopoietic stem transplant. Despite these promising results, challenges remain in administering Treg including the difficulty isolating pure Tregs expanding them to a therapeutic dose. We previously found thymus, which is routinely removed during pediatric cardiac surgery, plentiful source of Tregs. These be easily isolated, xenoGVHD mice....