A5027418175- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Nutrition and Health in Aging
- Muscle metabolism and nutrition
- Metabolism, Diabetes, and Cancer
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Cardiovascular and exercise physiology
- Exercise and Physiological Responses
- Cancer, Hypoxia, and Metabolism
- Cardiomyopathy and Myosin Studies
- Mesenchymal stem cell research
- Pancreatic function and diabetes
- Respiratory Support and Mechanisms
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Inflammasome and immune disorders
- GDF15 and Related Biomarkers
- Sarcoma Diagnosis and Treatment
- Nerve injury and regeneration
- Delphi Technique in Research
- Neuroscience of respiration and sleep
- biodegradable polymer synthesis and properties
- Muscle activation and electromyography studies
Inserm
2022-2025
Université Claude Bernard Lyon 1
2022-2025
Institut NeuroMyoGène
2022-2025
Centre National de la Recherche Scientifique
2022-2025
Maastricht University Medical Centre
2016-2019
Maastricht University
2018
Wageningen University & Research
2014
Significance Physical exercise causes profound changes in energy metabolism humans. In this study we show that resting skeletal muscle has a crucial role the metabolic response to acute exercise. During endurance exercise, selective induction of protein angiopoietin-like 4 (ANGPTL4) nonexercising reduces local fatty acid uptake, presumably prevent fat overload, while directing acids active as fuel. Our data thus suggest key governing lipid homeostasis during
Abstract The cellular mechanisms underlying axonal morphogenesis are essential to the formation of functional neuronal networks. We previously identified autism-linked kinase NUAK1 as a central regulator axon branching through control mitochondria trafficking. However, (1) relationship between mitochondrial position, function and (2) downstream effectors whereby regulates remain unknown. Here, we report that recruitment synaptic boutons supports collateral branches stabilization rather than...
Physical inactivity significantly contributes to loss of muscle mass and performance in bed-bound patients. Loss skeletal mitochondrial content has been well-established unloading models, but the underlying molecular mechanism remains unclear. We hypothesized that apparent unloading-induced is preceded by increased mitophagy- decreased biogenesis-signaling during early stages unloading.We analyzed a comprehensive set markers involved mitochondrial-autophagy, -biogenesis, -dynamics, -content,...
Loss of skeletal muscle mitochondrial oxidative capacity is well-established in patients with COPD, but the role breakdown herein largely unexplored. Currently, we studied if signalling increased COPD and associates loss content, whether it affected iron deficiency (ID) or systemic inflammation. Therefore, mitophagy, autophagy, dynamics content markers were analysed vastus lateralis biopsies (N = 95, FEV1% predicted: 39.0 [31.0-53.6]) healthy controls 15, 112.8 [107.5-125.5]). Sub-analyses...
Lipid metabolism is a key process required for muscle stem cell (MuSCs) function during regenerative myogenesis. However, the molecular pathway responsible of such regulation still unknown. Studies lysine demethylase PHF2 have reported its critical role in lipid pathological processes, although there are no data regarding MuSC fate transition. Here we show that controls droplet homeostasis MuSCs myogenesis, by promoting contact between droplets and mitochondria. Consistently, absence PHF2,...
Abstract Serotonin reuptake inhibitor antidepressants such as fluoxetine are widely used to treat mood disorders. The mechanisms of action include an increase in extracellular level serotonin, neurogenesis, and growth vessels the brain. We investigated whether could have broader peripheral regenerative properties. Following prolonged administration male mice, we showed that increases number muscle stem cells angiogenesis, associated with positive changes skeletal function. Fluoxetine also...
Chronic intermittent hypoxia (IH), a major feature of obstructive sleep apnea syndrome (OSA), is associated with greater severity myocardial infarction. In this study, we performed RNA sequencing cardiac samples from mice exposed to IH, which reveals specific transcriptomic signature the disease, relative mitochondrial remodeling and cell death. Corresponding its activation under chronic stabilized Hypoxia Inducible Factor‐1α (HIF‐1α) in cells vitro observed association an increased...
Abstract Background Pulmonary rehabilitation (PR) is a cornerstone in the management of chronic obstructive pulmonary disease (COPD), targeting skeletal muscle to improve functional performance. However, there substantial inter‐individual variability effect PR on performance, which cannot be fully accounted for by generic phenotypic factors. We performed an unbiased integrative analysis molecular responses COPD patients and comprehensively characterized their baseline physical function, body...
Abstract Exacerbations in Chronic obstructive pulmonary disease (COPD) are often accompanied by and systemic inflammation, associated with an increased susceptibility to weight loss muscle wasting. As the emphysematous phenotype COPD appears prone skeletal wasting, aims of this study were evaluate compared control mice following repetitive exacerbations (1) changes mass strength and, (2) whether recovery its underlying processes impaired. Emphysema was induced intra-tracheal (IT) elastase...
Due to the post-mitotic nature of skeletal muscle fibers, adult maintenance relies on dedicated stem cells (MuSCs). In most physiological contexts, MuSCs support myofiber homeostasis by contributing myonuclear accretion, which requires a coordination cell-type specific events between and MuSCs. Here, we addressed role kinase AMPKα2 in these supporting accretion. We demonstrate that deletion impairs regeneration. Through vitro assessments MuSC myogenic fate EdU-based cell tracing, reveal...
Skeletal muscle regeneration after disuse is essential for maintenance and involves the regulation of both mass- metabolic plasticity-related processes. However, relation between these processes during recovery from remains unclear. In this study, we explored potential interrelationship molecular mass oxidative metabolism disuse. Molecular profiles were measured in biopsies vastus lateralis healthy men 1-leg cast immobilization 1 wk reloading, mouse gastrocnemius obtained before hindlimb...
Due to the post-mitotic nature of myonuclei, postnatal myogenesis is essential for skeletal muscle growth, repair, and regeneration. This process facilitated by satellite cells through proliferation, differentiation, subsequent fusion with a pre-existing fiber (i.e., myonuclear accretion). Current knowledge primarily based on in vitro formation syncytia from myoblasts, which represents aspects developmental myogenesis, but may incompletely portray myogenesis. Therefore, we aimed develop an...
ABSTRACT Rhabdomyosarcoma (RMS) is the most frequent form of paediatric soft-tissue sarcoma and remains a medical challenge, holding in failure current therapeutic strategies. RMS shares histological features with cells muscle lineage this cancer thought to arise from malignant transformation myogenic precursors. It has been proposed that myogenesis could represent “Jekyll Hyde” skeletal muscle. The underlying idea some early steps differentiation are blocked RMS, understanding how normal...
<b>Background:</b> Pulmonary rehabilitation (PR) is a cornerstone in the management of COPD, targeting skeletal muscle to improve exercise performance. However, there substantial inter-individual variability effect PR on performance, which cannot be fully accounted for by generic phenotypic factors. To identify groups with differential response, we performed an unbiased integrative analysis molecular responses COPD patients. <b>Methods:</b><i>M. Quadriceps</i> biopsies were obtained from 51...
ABSTRACT The value of the Cre-lox system in biology is well-recognized, which reflected by its widespread use to assess role a gene specific tissue or cell-type. Not least, Cre recombinase expressed under Pax7 promotor has been invaluable for study skeletal muscle stem cell (MuSC) biology. In this study, we aimed systematically effects genetic makeup Pax7Cre mice and Tamoxifen (Tx) treatment on regeneration. We demonstrate that Tx per se does not affect regeneration at 14 days post injury...
Abstract Muscular dystrophies, such as Duchenne muscular dystrophy (DMD), are caused by permanent muscle injuries leading to chronic inflammation. In that context, macrophages harbor an altered inflammatory profile contributes fibrosis through the secretion of profibrotic cytokine TGFβ1. We previously showed AMP-activated protein kinase (AMPK) activation reduces TGFβ1 and improves homeostasis force in a mouse model DMD. This makes AMPK attractive therapeutic target for treating inflammation...
Abstract Chronic intermittent hypoxia (IH), a major feature of obstructive sleep apnea syndrome (OSA), is associated with more severe myocardial infarction. In this study, we performed RNA sequencing cardiac samples from mice exposed to IH, which reveals specific transcriptomic signature the disease, relative mitochondrial remodeling and cell death. Corresponding its activation under chronic stabilized Hypoxia Inducible Factor-1α (HIF-1α) in cells vitro, observed association an increased...
ABSTRACT Skeletal muscle is a highly organized tissue that possesses the ability to contract and exerts metabolic, endocrine paracrine functions. Previous studies explored these functions through in vitro models, computational analyses, endpoint measurements. To include cellular complexity modulates functions, allow us study kinetics of released and/or depleted factors, we developed novel setup for ex vivo contraction whole skeletal muscle. Ex Extensor Digitorum Longus (EDL) induced by...