A5023202611- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Connexins and lens biology
- Retinal Imaging and Analysis
- Carbohydrate Chemistry and Synthesis
- Enzyme Catalysis and Immobilization
- Proteoglycans and glycosaminoglycans research
- Horticultural and Viticultural Research
- Biomarkers in Disease Mechanisms
- Yeasts and Rust Fungi Studies
- Fermentation and Sensory Analysis
- Intraocular Surgery and Lenses
- Photosynthetic Processes and Mechanisms
- Glaucoma and retinal disorders
- Cell Adhesion Molecules Research
- Complement system in diseases
- Glycogen Storage Diseases and Myoclonus
- Inflammasome and immune disorders
- Fibroblast Growth Factor Research
- Advanced Cellulose Research Studies
- Mercury impact and mitigation studies
- Oral and gingival health research
- Connective tissue disorders research
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
University of Manchester
2014-2021
Engineering and Physical Sciences Research Council
2020
Wellcome Centre for Cell-Matrix Research
2013-2018
Manchester Academic Health Science Centre
2018
Little is known about the impact of viral infections on lung matrix despite its important contribution to mechanical stability and structural support. The composition also indirectly controls inflammation by influencing cell adhesion, migration, survival, proliferation differentiation. Hyaluronan a significant component extracellular production degradation must be carefully balanced. We have discovered an imbalance in hyaluronan following resolution severe influenza virus infection, driven...
Abstract Age-related macular degeneration (AMD) is a leading cause of blindness. Genetic variants at the chromosome 1q31.3 encompassing complement factor H ( CFH , FH) and related genes CFHR1-5 ) are major determinants AMD susceptibility, but their molecular consequences remain unclear. Here we demonstrate that FHR-4 plays prominent role in pathogenesis. We show systemic levels elevated P -value = 7.1 × 10 −6 ), whereas no difference seen for FH. Furthermore, accumulates choriocapillaris,...
Mammalian oocytes are surrounded by a highly hydrated hyaluronan (HA)-rich extracellular matrix with embedded cumulus cells, forming the cell·oocyte complex (COC) matrix. The correct assembly, stability, and mechanical properties of this matrix, which crucial for successful ovulation, transport COC to oviduct, its fertilization, depend on interaction between HA specific HA-organizing proteins. Although proteins inter-α-inhibitor (IαI), pentraxin 3 (PTX3), TNF-stimulated gene-6 (TSG-6) have...
Fatty acid photodecarboxylases (FAP) are a recently discovered family of FAD-containing, light-activated enzymes, which convert fatty acids to n-alkanes/alkenes with potential applications in the manufacture fine and speciality chemicals fuels. Poor catalytic stability FAPs is however major limitation. Here, we describe methodology purify catalytically stable homogeneous samples recombinant Chlorella variabilis NC64A FAP (CvFAP) from Escherichia coli. We demonstrate that blue light-exposure,...
Under inflammatory conditions and in the matrix of cumulus-oocyte complex, polysaccharide hyaluronan (HA) becomes decorated covalently with heavy chains (HCs) serum glycoprotein inter-α-inhibitor (IαI). This alters functional properties HA as well its structural role within extracellular matrices. The covalent transfer HCs from IαI to is catalyzed by TSG-6 (tumor necrosis factor-stimulated gene-6), but also known a cross-linker that induces condensation matrix. Here, we investigate interplay...
Age-related macular degeneration (AMD) is the leading cause of blindness in western world and affects nearly 200 million people globally. Local inflammation driven by complement system dysregulation currently a therapeutic target. Bruch's membrane (BrM) sheet extracellular matrix that separates retina from underlying choroid, highly vascularized layer supplies oxygen nutrition to outer retina. Here we show most proteins are unable diffuse through BrM, although FHL-1, factor D C5a can....
Age-related cataract formation is the primary cause of blindness worldwide and although treatable by surgical removal lens majority sufferers have neither finances nor access to medical facilities required. Therefore, a better understanding pathogenesis may identify new therapeutic targets prevent or slow its progression. Cataract incidence strongly correlated with age cigarette smoking, factors that are often associated accumulation metal ions in other tissues. Therefore this study...
Enzyme-CBM fusion constructs immobilised on wood-derived cellulose scaffolds: a sustainable approach for continuous flow biocatalysis.
Abstract Retinal inflammation plays a key role in the progression of age-related macular degeneration (AMD), condition that leads to loss central vision. The deposition acute phase pentraxin C-reactive protein (CRP) macula activates complement system, thereby contributing dysregulated inflammation. factor H (FH) can bind CRP and down-regulate an inflammatory response. However, it is not known whether truncated form FH, called H-like 1 (FHL-1), which significant regulatory eye, also interacts...
Purpose: Choroidal endothelial cells play a central role in the pathogenesis of age-related macular degeneration (AMD). Protocols for isolating primary choroidal have been described but require access to human donor eyes, which is limiting factor. Therefore, conditionally immortalized cell (ciChEnC) line has established. Methods: were selected by magnetic-activated sorting and using temperature-sensitive simian virus 40 large T antigen telomerase. The obtained was characterized based on...
Genetic admixture can provide material for populations to adapt local environments, and this process has played a crucial role in the domestication of plants animals. The model yeast, Saccharomyces cerevisiae, been domesticated multiple times production wine, sake, beer, bread, but high rate between yeast lineages so far treated as complication population genomic analysis. Here, we make use low recombination at centromeres investigate using classic Bayesian approach locus-by-locus...
Abstract Retinal pigment epithelial (RPE) cells that underlie the neurosensory retina are essential for maintenance of photoreceptor and hence vision. Interactions between RPE their basement membrane, i.e. inner layer Bruch’s cell health function, but signals induced by membrane engagement, contributions to fate determination remain poorly defined. Here, we studied functional role soluble complement regulator component Factor H-like protein 1 (FHL-1). Human primary adhered FHL-1 in a manner...
Glycogen storage disease type Ib (GSD Ib) [MIM 232220] is caused by mutations in the gene encoding microsomal glucose-6-phosphate translocase (G6PT) (Veiga-Da-Cunha et al, 1998). This solute carrier family 37, member 4 (SLC37A4) located on chromosome 11q23.3 and encodes a 429 amino acid polypeptide protein transports into endoplasmic reticulum, where enzyme glucose-6-phosphatase converts glucose inorganic phosphate. Mutations SLC37A4 result accumulation of cells. The has 9 exons so far 83...
Abstract Retinal pigment epithelial (RPE) cells that underlie the neurosensory retina are essential for maintenance of photoreceptor and hence vision. Interactions between RPE their basement membrane, i.e . inner layer Bruch’s cell health function, but signals induced by membrane engagement, contributions to fate determination remain poorly defined. Here, we studied functional role soluble complement regulator component Factor H-like protein 1 (FHL-1). Human primary adhered FHL-1 in a manner...
Abstract Genetic admixture can provide material for populations to adapt local environments, and this process has played a crucial role in the domestication of plants animals. The model yeast, Saccharomyces cerevisiae , been domesticated multiple times production wine, sake, beer bread, but high rate between yeast lineages so far treated as complication population genomic analysis. Here we make use low recombination at centromeres investigate using classic Bayesian approach more conservative...