A5068584403- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- bioluminescence and chemiluminescence research
- Biosensors and Analytical Detection
- Cystic Fibrosis Research Advances
- Bacterial Identification and Susceptibility Testing
- Antimicrobial agents and applications
- Ocular Infections and Treatments
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Molecular Biology Techniques and Applications
- Dental Research and COVID-19
- Antibiotic Use and Resistance
- Orthopedic Infections and Treatments
- Surgical Sutures and Adhesives
- Antifungal resistance and susceptibility
- Advanced Polymer Synthesis and Characterization
- Bacteriophages and microbial interactions
- Infection Control in Healthcare
- Photoacoustic and Ultrasonic Imaging
- Surgical site infection prevention
- Skin Protection and Aging
- Pneumonia and Respiratory Infections
University Hospital Regensburg
2012-2024
Ludwig-Maximilians-Universität München
2014
Institute of Medical Microbiology and Hygiene
2009
Background. In patients with cystic fibrosis (CF), the emergence of hypermutable Pseudomonas aeruginosa drives selection P. variants that are efficiently adapted to inflamed lungs these patients.
Photodynamic inactivation of bacteria (PIB) proves to be an additional method kill pathogenic bacteria. PIB requires photosensitizer molecules that effectively generate reactive oxygen species like singlet when exposed visible light. To allow a broad application in medicine, photosensitizers should safe applied humans. Substances vitamin B2, which are most likely safe, known produce upon irradiation. In the present study, we added positive charges flavin derivatives enable attachment these...
In most of Europe and especially in Germany, there is currently a concerning rise the number hospital-acquired infections due to vancomycin-resistant Enterococcus faecium (VREfm). Therefore, need improve our understanding way VREfm spreads hospitals. this study, we investigated molecular epidemiology isolates from first appearance at university hospital 2004 until 2010. There only very scarce information about early time Germany. Our analysis includes all available each patient tertiary care...
The isolation of affected patients and intensified infection control measures are used to prevent nosocomial transmission vancomycin-resistant enterococci (VRE), but early detection VRE carriers is needed. However, there still no standard screening criteria for VRE, which poses a significant threat patient safety. Our study aimed develop evaluate an artificial intelligence (AI)-based approach identifying predicting at-risk who could assist prevention staff through human-in-the-loop approach....
Photodynamic inactivation of bacteria (PIB) is based on photosensitizers which absorb light and generate reactive oxygen species (ROS), killing cells via oxidation. PIB evaluated by comparing viability with without irradiation, where reduction in the presence photosensitizer irradiation considered as dark toxicity. This effect controversially discussed for like TMPyP (5,10,15,20-Tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluensulfonate). shows a high absorption coefficient blue yield...
Abstract Highly resistant endospores may cause severe problems in medicine as well the food and packaging industries. We found that bacterial can be inactivated quickly with reactive oxygen species (ROS) were generated by a new generation of flavin photosensitizers. Flavins like natural compound vitamin B2 are already known to produce ROS but they show poor antimicrobial photodynamic killing efficacy due lack positive charges. Therefore we synthesized photosensitizers have one (FLASH-01a) or...
The colonization of skin with pathogenic, partially antibiotic-resistant bacteria is frequently a severe problem in dermatological therapies. For instance, Staphylococcus aureus even disease-promoting factor atopic dermatitis. photodynamic inactivation (PDI) could be new antibacterial procedure. Upon irradiation visible light, special photosensitizer exclusively generates singlet oxygen. This reactive oxygen species kills via oxidation independent or strain and their antibiotic resistance...
To prevent infections that arise from the skin surface it is necessary to decolonize human prior any proposed treatment or surgical intervention. Photodynamic inactivation of bacteria (PIB) uses cationic photosensitizers attach bacteria, generate reactive oxygen species on light irradiation and thereby kill via oxidative mechanisms.To evaluate potential safety PIB for decolonization skin.PIB with new photosensitizer SAPYR [2-((4-pyridinyl)methyl)-1H-phenalen-1-one chloride] was initially...
Today, there is a continuous worldwide battle against antimicrobial resistance (AMR) and that includes vancomycin-resistant enterococci (VRE). Methods can adequately quickly detect transmission chains in outbreaks are needed to trace manage this problem fast cost-effectively. In study, DNA-microarray-based technology was developed for purpose. It commenced with the bioinformatic design of specific oligonucleotide sequences obtain amplification primers hybridization probes. Microarrays were...
Photodynamic inactivation of microorganisms (PDI) finds use in a variety applications. Several studies report on substances enhancing or inhibiting PDI. In this study, we analyzed the inhibitory potential ubiquitous salts like CaCl2 and MgCl2 PDI against Staphylococcus aureus Pseudomonas aeruginosa cells using five cationic photosensitizers methylene blue, TMPyP, SAPYR, FLASH-02a FLASH-06a. TMPyP changed its molecular structure when exposed to MgCl2, most likely due complexation....
Photodynamic inactivation (PDI) of pathogenic bacteria is a promising technology in different applications. Thereby, photosensitizer (PS) absorbs visible light and transfers the energy to oxygen yielding reactive species (ROS). The produced ROS are then capable killing microorganisms via oxidative damage cellular constituents. Among other PS, some flavins producing cationic already successfully applied PDI. When PDI used for example on tap water, PS like will encounter various ions small...
Many studies show that photodynamic inactivation (PDI) is a powerful tool for the fight against pathogenic, multiresistant bacteria and closing of hygiene gaps. However, PDI have been frequently performed under standardized in vitro conditions comprising artificial laboratory settings. Under real-life conditions, however, encounters substances like ions, proteins, amino acids fatty acids, potentially hampering efficacy to an unpredictable extent. Thus, we investigated with...
One of the sources infections in hospitals is presence potentially harmful bacteria on skin surface (carriage) a patient without development an infection, but which can spread to other people under certain conditions. Bacteria living also contribute worsening eczema. The current methods ridding these microbes (bacteria) involves giving either topical (on skin) or oral (by mouth) antibiotics, strategy which, over time, thought have caused antibiotic resistance, meaning antibiotics no longer...
Abstract The antibiotic crisis increasingly threatens the health systems world-wide. Especially as there is an innovation gap in development of novel antibiotics, treatment options for bacterial infections become fewer. photodynamic inactivation (PDI) bacteria appears to be a potent, new technology that may support colonized or infected skin. In inactivation, dye – called photosensitizer absorbs light and generates reactive singlet oxygen. This oxygen then capable killing independent species...
作为医院感染的来源之一, 患者皮肤表面(载体)存在潜在有害细菌, 它们不会产生感染, 但在某些条件下可能传播给其他人。皮肤表面生活的细菌也会导致湿疹恶化。目前去除这些微生物(细菌)皮肤的方法包括给患者使用局部抗生素(皮肤上的)或口服抗生素(口服),但随着时间的推移, 这种策略被认为导致了抗生素耐药性, 这意味着抗生素不再能杀死细菌。本文中描述的是由德国雷根斯堡大学的一个小组进行的研究。他们使用切掉的猪和人体皮肤样本, 在样本中加入耐药细菌,如耐甲氧西林葡萄球菌或 MRSA, 然后用一种化学溶液对皮肤进行处理, 这种化学溶液的作用是增加对光的敏感性。皮肤样本随后暴露在光源下,这一过程被称为光动力激活。他们发现这种技术可以在不损害皮肤细胞的情况下, 大量减少细菌的数量。作者建议,这一过程现在应该作为一种更安全的方法来研究, 以减少表面细菌运输, 而不增加细菌耐药性的风险,也不损害皮肤。
The photodynamic inactivation (PDI) uses molecules (photosensitizers) that absorb visible light energy, transfer it to adjacent molecular oxygen and thereby generating the biocidal singlet in situ . PDI was tested against Listeria monocytogenes Salmonella enterica suspensions, on inoculated surfaces using spray suspensions antimicrobial coatings (AMC), without with soiling. In suspension, reduced number of viable more than 6 log 10 steps within seconds or minutes exposure. use a...