A5063692384- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Genetic diversity and population structure
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Orthoptera Research and Taxonomy
- Chromosomal and Genetic Variations
- Plant and Fungal Interactions Research
- Banana Cultivation and Research
- Cocoa and Sweet Potato Agronomy
- Animal Behavior and Reproduction
- Cassava research and cyanide
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Evolution and Genetic Dynamics
- Neurobiology and Insect Physiology Research
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Insect-Plant Interactions and Control
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Ecology and Vegetation Dynamics Studies
- Phytoplasmas and Hemiptera pathogens
- Insect Resistance and Genetics
- Heat shock proteins research
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2014-2024
Université de Montpellier
2019-2024
Centre Occitanie-Montpellier
2021-2024
Institut de Recherche pour le Développement
2021-2024
Institut Agro Montpellier
2019-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
L'Institut Agro
2020-2024
Plant Health Institute de Montpellier
2021-2024
Biologie et Génétique des Interactions Plante-Parasite
2010-2020
Advanced Media Research (United States)
2016
The Australian plague locust, Chortoicetes terminifera, is among the most promising species to unravel suites of genes underling density-dependent shift from shy and cryptic solitarious behaviour highly active aggregating gregarious that characteristic locusts. This because it lacks many major phenotypic changes in colour morphology accompany phase change other locust species. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) sensitive method available for determining...
Next-generation sequencing (NGS), through the implementation of metagenomic protocols, has led to discovery thousands new viruses in last decade. Nevertheless, these protocols are still laborious and costly implement, technique not yet become routine for everyday virus characterization. Within context CRESS DNA studies, we implemented two alternative long-read NGS one that is agnostic sequence (without a priori knowledge viral genome) other use specific primers target (with priori). Agnostic...
ABSTRACT Endogenous plant pararetroviruses (EPRVs) are viral sequences of the family Caulimoviridae integrated into nuclear genome numerous species. The ability some endogenous Banana streak viruses (eBSVs) in banana ( Musa sp.) to induce infections just like virus itself was recently demonstrated (P. Gayral et al., J. Virol. 83:6697-6710, 2008). Although eBSVs probably arose from accidental events, infectious constitute an extreme case parasitism, as well a newly described strategy for...
Abstract Between plagues, the solitarious desert locust ( Schistocerca gregaria ) is generally thought to exist as small populations, which are particularly prone extinction events in arid regions of Africa and Asia. Given high genetic structuring observed one geographical area (the Eritrean coast) by former authors, a metapopulation dynamics model involving repeated colonization was favoured. In this study, we assessed validity demographic scenario temporary populations phase analysing...
This study addresses geographic variation of body size and shape allele frequencies at 21 microsatellite loci in southern African populations the desert locust, Schistocercagregaria. These populations, which belong to subspecies S. g. flaviventris, lack capacity change phase swarm relative northern nominate subspecies, gregaria. We reported overall genetic morphological similarities among localities that cover most range. Of particular interest, level diversity was moderately lower than...
Abstract Unravelling variation among taxonomic orders regarding the rate of evolution in microsatellites is crucial for evolutionary biology and population genetics research. The mean mutation tends to be lower arthropods than vertebrates, but data are scarce mostly concern accumulation mutations model species. Based on parent–offspring segregations a hierarchical Bayesian model, orthopteran insect Schistocerca gregaria was estimated at 2.1e −4 per generation untranscribed dinucleotide...
Abstract We evaluated the validity of subspecific designation for Schistocerca gregaria (Forskål) and flaviventris (Burmeister), isolated in distinct regions along north–south axis Africa. Towards this goal, we assessed variation multiple morphological molecular traits within species. first used elliptic Fourier landmark‐based relative warps analyses to compare size shape two internal external structures male genitalia. provide a discriminant function which classified specimens with 100%...
Dating population divergence within species from molecular data and relating such dating to climatic biogeographic changes is not trivial. Yet it can help formulating evolutionary hypotheses regarding local adaptation future responses changing environments. Key issues include statistical selection of a demographic historical scenario among set possible scenarios, estimation the parameter(s) interest under chosen scenario. Such inferences greatly benefit (a) independent information on rate...
Abstract The desert locust ( Schistocerca gregaria ) has been feared agricultural pest since early civilization, with plagues documented in ancient texts. Population genetic studies of the are needed to determine variation and movement pattern for efficient control pest. In this study, we complemented limited available microsatellite collection 34 new polymorphic multiplexed loci. To aim, screened an expressed sequence tags library constructed a partial genomic enriched dinucleotide repeats...
In recent years, Brown spot disease of rice (BSR) has been observed on leaves and seeds in all rice-growing areas Burkina Faso. Bipolaris oryzae Exserohilum rostratum are the main fungal species isolated from BSR infected tissues they frequently same field. However, we lacking information genetic diversity population structure these fungi The mode reproduction is also unknown. isolates B. (n=61) E. (n=151), collected major Faso, was estimated using genotyping-by-sequencing (GBS). mean values...
We conducted a comparative population genetic analysis of levels variation and its geographical structuring in three closely related species grasshopper that co-occur the Mediterranean Basin: Calliptamus italicus, C. barbarus wattenwylianus.In western part their distributions 5 populations 13 10 wattenwylianus were sampled.Bootstrap re-sampling microsatellite loci within each indicated lower level diversity higher differentiation barbarus, which is less an outbreak pest than either other...
Diverse molecular markers have been used to analyze the genetic diversity of plant pathogens. Compared with traditional fingerprinting methods, multiple loci variable number tandem repeat analyses (MLVAs) gained importance recently due their reproducibility, high discriminatory power, ease performance, low cost, and throughput potential. These characteristics are desirable for continuous pathogen monitoring, especially pathogens relatively diversity, disease epidemiology studies. Genetic...
Abstract Now that it has been realized viruses are ubiquitous, questions have raised on factors influencing their diversity and distribution. For phytoviruses, understanding the interplay between plant virus species richness prevalence remains cardinal. As both amplification dilution of viral due to increasing host theorized observed, a deeper how plants interact in natural environments is needed explore availability conditions distributions. From unique dataset, this study explores...
In insects, extra-molting has been viewed as a compensatory mechanism for nymphal growth that contributes to optimize body weight successful reproduction. However, little is known on the capacity of evolve in natural populations, which limits our understanding how selection acts growth. We used multi-generational pedigree, individual monitoring and quantitative genetics models investigate evolution its impact solitarious population desert locust, Schistocerca gregaria. Growth compensation...
The Calliptamus genus (Orthoptera: Acrididae) includes locust and grasshopper species, some of which have a high economic impact. Using an enriched methodology, 10 microsatellite markers been developed from two italicus barbarus. These polymorphic were tested on different populations three species: C. italicus, barbarus, wattenwylianus. Two amplified the as well four barbarus italicus. In each 9 to 23 alleles per locus observed. molecular might prove be new interesting tool for population...
Xanthomonas vasicola pv. musacearum (Xvm) which causes wilt (XW) on banana (Musa accuminata x balbisiana) and enset (Ensete ventricosum), is closely related to the species that contains pathovars vasculorum (Xvv) holcicola (Xvh), respectively pathogenic sugarcane sorghum. Xvm considered a monomorphic bacterium whose intra-pathovar diversity remains poorly understood. With sudden emergence of within east central Africa coupled with unknown origin one two sublineages suggested for Xvm,...
Southern rice black-streaked dwarf virus (SRBSDV), which causes severe disease symptoms in (Oriza sativa L.) has been emerging the last decade throughout northern Vietnam, southern Japan and southern, central eastern China. Here we attempt to quantify prevalence of SRBSDV Honghe Hani terraces system (HHRTS)—a Chinese 1300-year-old traditional production system. We first confirm that genetically diverse varieties are still being cultivated HHRTS categorize these into three main genetic...
Abstract Tychoparthenogenesis, a form of asexual reproduction in which small proportion unfertilized eggs can hatch spontaneously, could be an intermediate evolutionary link the transition from sexual to parthenogenetic reproduction. The lower fitness tychoparthenogenetic offspring due either developmental constraints or inbreeding depression more homozygous individuals. We tested hypothesis that populations where has been purged, tychoparthenogenesis may less costly. To assess this...
The Honghe Hani rice terraces system (HHRTS) is a traditional cultivation where people cultivate remarkably diverse varieties. Recent introductions of modern varieties to the HHRTS have significantly increased severity diseases within terraces. Here, we determine impacts these recent on composition rice-associated microbial communities. We confirm that contains range both landraces and introduced find differences between communities two groups. However, this introduction has not strongly...
Abstract Definition of the genus Calliptamus (Orthoptera: Acrididae) has generated many taxonomic debates. Even now, existence different geographical morphs hinders species determination, particularly as concerns females and larvae. Some these are observed in southern France recognized potential pests. To circumvent problems identification ecological surveys, we developed a single multiplex PCR method based on mitochondrial Cytochrome Oxydase I diagnostic polymorphisms to differentiate...
Cassava Bacterial Blight (CBB) is a destructive disease widely distributed in the different areas where this crop grown. Populations studies have been performed at local and national scales revealing geographical genetic structure with temporal variations. A global epidemiology analysis of its causal agent Xanthomonas phaseoli pv. manihotis (Xpm) needed to better understand expansion for improving monitoring CBB. We targeted new tandem repeat (TR) loci large units, i.e. minisatellites, that...
Mirids are the primary pests affecting cocoa production in Africa, but no genetic studies have been conducted on these insects. Here we report isolation and characterization of 12 polymorphic microsatellite loci for Sahlbergella singularis. A microsatellite-enriched genomic DNA library was developed screened to identify marker loci. Twelve were identified by screening 28 individuals collected from one presumed population plantations Southern Cameroon. The number alleles ranged 5 25, whereas...