Abstract Background Diabetic wounds threaten the health and quality of life patients their treatment remains challenging. ADSC-derived exosomes have shown encouraging results in enhancing diabetic wound healing. However, how to use effectively is a problem that needs be addressed at present. Methods A mouse skin model was established. (ADSC-Exos) were isolated, vitro application evaluated using human umbilical vein endothelial cells (HUVECs) dermal fibroblasts (HDFs). After preparation...

Reduction of the survival motor neurons (SMN) protein levels causes neuron degenerative disease spinal muscular atrophy, severity which correlates with extent reduction in SMN. SMN, together Gemins 2 to 7, forms a complex that functions assembly small nuclear ribonucleoprotein particles (snRNPs). Complete depletion SMN from cell extracts abolishes snRNP assembly, formation heptameric Sm cores on snRNAs. However, what effect, if any, levels, as occurs atrophy patients, has capacity cells...

Violence and injury risk behaviors, alcohol drug use problems, posttraumatic stress disorder (PTSD) depressive symptoms occur frequently among adolescents presenting to acute care medical settings after traumatic physical injury.To test the effectiveness of a stepped collaborative intervention targeting this constellation behaviors in randomly sampled hospitalized with without brain injury.A pragmatic randomized clinical trial was conducted at single US level I trauma center. Participants...

Human dermal papilla (DP) cells have been studied extensively when grown in the conventional monolayer. However, because of great deviation from real vivo three-dimensional (3D) environment, these two-dimensional (2D) tend to lose hair-inducible capability during passaging. Hence, 2D caused concerns motivated development novel 3D culture techniques produce cellular microtissues with suitable mimics. The hanging-drop approach is based on surface tension-based technique and interaction between...

Mesenchymal stem/stromal cells (MSCs) are a promising tool for cell-based therapies in the treatment of tissue injury. The stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis plays significant role directing MSC homing to sites However vivo distribution following intravenous transplantation remains poorly understood, potentially hampering precise prediction and evaluation therapeutic efficacy.A murine model partial ischemia/reperfusion (I/R) is used induce liver...

Hair regeneration has long captured researchers' attention because alopecia is a common condition and current therapeutic approaches have significant limitations. Dermal papilla (DP) cells serve as signaling center in hair follicles regulate formation cycling by paracrine secretion. Secreted EVs are important mediators for intercellular communication, DP-derived extracellular vesicles (DP-EVs) may play an role regeneration. However, the instability of vivo their low long-term retention after...

Abstract Background Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults. Ibrutinib, a Bruton’s tyrosine kinase (BTK) inhibitor, novel anticancer drug used for treating several types of cancers. In this study, we aimed to determine role ibrutinib on GBM. Methods Cell proliferation was determined by using cell viability, colony formation, 5-ethynyl-2′-deoxyuridine (EdU) assays. cycle apoptosis were analyzed flow cytometry. migratory ability evaluated wound...

Abstract Morphogenesis of hair follicles during development and in reconstitution assays involves complex interactions between epithelial cells dermal papilla (DPCs). DPCs may be a source for regeneration alopecia patients. Reconstitution engineered requires vitro culture trichogenic cells, three-dimensional scaffolds, biomolecular signals. However, tend to lose their biological activity when cultured as scaffolds currently used follicle lack efficiency biocompatibility. Platelet-rich plasma...

The survival of motor neurons (SMN) complex is essential for the biogenesis small nuclear ribonucleoprotein (snRNP) complexes in eukaryotic cells. Reduced levels SMN cause neuron degenerative disease, spinal muscular atrophy. We identify here stable subunits that do not contain SMN. Sedimentation and immunoprecipitation experiments using cell extracts reveal at least three composed Gemin3, -4, -5; Gemin6, -7, unrip; with Gemin2, as well free Gemin5. Complexes containing Gemin3-Gemin4-Gemin5...

Abstract Cell surface engineering technology advances cell therapeutics and tissue by accurate micro/nanoscale control in cell–biomaterial ensembles spheroids formation. By tailoring surface, microgels can encapsulate cells for versatile uses. However, are coated a thick layer to house multiple together but not single based. Besides, excessive deposition on is detrimental cellular functions. Herein, layer‐by‐layer (LbL) self‐assembly using nanogel reported, owing its security tunable...

The restoration of hair-inductive potential in human dermal papilla cells (hDPCs) is a tremendous challenge for hair regeneration. Much the research thus far has indicated that three-dimensional (3-D) culture shows improved efficacy follicle (HF) neogenesis. However, mature HF cannot regenerate an incomplete microenvironment. This study developed optimized 3-D co-culture system to restore characteristics hDPCs by mimicking in-vivo As result, Matrigel-encapsulated spontaneously formed into...

Hair regenerative medicine, a promising strategy for the treatment of hair loss, will likely involve transplantation autologous follicular stem cells (HFSCs) and dermal papilla (DPCs) into regions loss. Cyclic regeneration results from periodic partial activation HFSCs. However, previous studies have not successfully achieved large-scale HFSC expansion in vitro without use feeder cells, with lack research focused on regulating fate (HF) regeneration. Hence, an emerging focus medicine is...

The dermal papilla cell (DPC) is a type of highly specialized mesenchymal cells located in hair follicles (HF). Due to the primary role epithelial-mesenchymal interaction that enables hair-follicle morphogenesis and cycling, DPC has become an attractive source for regeneration treat alopecia patients. However, DPCs tend lose their function during vitro culture. Hence, there exists clear need develop microenvironment can recapitulate interactions within native milieu DPCs. Since...

The application of dermal papilla cells to hair follicle (HF) regeneration has attracted a great deal attention. However, cultured (DPCs) tend lose their capacity induce growth during passage, restricting usefulness. Accumulating evidence indicates that DPCs regulate HF mainly through unique paracrine properties, raising the possibility therapies based on extracellular vesicles (EVs). In this study, we explored effects EVs from high- and low-passage human scalp (DP-EVs) activation growth,...

Platelet rich plasma (PRP) has been accepted as a potential therapy for treating androgenetic alopecia (AGA).To fully clarify the underling molecular mechanisms of PRP action on hair growth and promote its clinical applications.In this study, we used mice models protein biochip to explore specific regulating growth. Then, performed randomized, placebo-controlled, double-blind, half-head study 52 AGA patients verify therapeutic efficacy in Chinese patients.The results confirmed that treatment...

The motor neuron degenerative disease spinal muscular atrophy is caused by reduced expression of the survival (SMN) protein. Here we report a genetic system developed in chicken pre-B cell line DT40, which endogenousSMN gene disrupted homologous recombination, and SMN protein expressed from cDNA under control tetracycline (tet)-repressible promoter. Addition tet results depletion consequent death, directly demonstrates that required for viability. tet-induced lethality can be rescued human...

Resection of the primary tumor is recommended for symptom relief in de novo stage IV breast cancer. We explored whether local surgery could provide a survival benefit these patients and attempted to characterize population that from surgery.Metastatic Breast cancer (N = 313) with intact between January 2006 April 2013 were separated into two groups according or not they had undergone surgery. The difference characteristics was analyzed using chi-square test, Fisher's exact test Mann-Whitney...

Background: It has been reported that collagenases (matrix metalloproteinase 2 [MMP-2] and matrix 9 [MMP-9]) are associated with hair cycle, whereas the mechanism of association is largely unknown. Methods: The mice were randomly allocated into four groups: saline, 5, 10, 15 nM SB-3CT. Immunohistochemical analysis was employed to examine MMP-2 MMP-9 protein. Real-time polymerase chain reaction enzyme-linked immunosorbent assay performed determine mRNA protein levels VEGF, IGF-1, TGF-β, GAPDH...