A5058521842- PI3K/AKT/mTOR signaling in cancer
- Nanoplatforms for cancer theranostics
- CAR-T cell therapy research
- Nanoparticle-Based Drug Delivery
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Nanowire Synthesis and Applications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cytokine Signaling Pathways and Interactions
- Biosimilars and Bioanalytical Methods
- Mast cells and histamine
- bioluminescence and chemiluminescence research
- Nanoparticles: synthesis and applications
- Extracellular vesicles in disease
- Chemokine receptors and signaling
- Bioactive Compounds and Antitumor Agents
- Immune cells in cancer
- Photodynamic Therapy Research Studies
- NF-κB Signaling Pathways
- Phagocytosis and Immune Regulation
- Advanced Drug Delivery Systems
American Type Culture Collection
2022-2024
Korea Institute of Science and Technology
2015-2023
Dana-Farber Cancer Institute
2019-2022
Harvard University
2019-2022
Seoul National University Hospital
2019
Korea University of Science and Technology
2013-2016
Korea Research Institute of Bioscience and Biotechnology
2013-2015
Johns Hopkins Medicine
2006
Johns Hopkins University
2006
Abstract The non-invasive photodynamic therapy has been limited to treat superficial tumours, primarily ascribed poor tissue penetration of light as the energy source. Herein, we designed a long-circulating hydrophilized titanium dioxide nanoparticle (HTiO 2 NP) that can be activated by ultrasound generate reactive oxygen species (ROS). When administered systemically mice, HTiO NPs effectively suppressed growth tumours after treatments. In tumour tissue, levels proinflammatory cytokines were...
Establishment of an appropriate cell labeling and tracking method is essential for the development cell-based therapeutic strategies. Here, we are introducing a new by combining metabolic gylcoengineering bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-d-mannosamine (Ac4ManNAz) to generate unnatural azide groups (-N3) on surface cells. Subsequently, specifically conjugated near-infrared fluorescent (NIRF) dye-tagged dibenzyl...
Abstract Interferon-gamma (IFN-γ), a cytokine critical for activating cellular immunity and facilitating anti-tumor responses, has become key assessing the efficacy of cancer immunotherapy drugs. While enzyme-linked immunosorbent assay (ELISA) is widely employed detection IFN-γ, it shown significant limitations in detecting low levels IFN-γ during early-stage immune activation. Moreover, presents serious challenges accurately quantifying paracrine signaling secreted by cells within 3-D...
While immunotherapies targeting T cells have achieved remarkable success, a significant subset of patients remain unresponsive or experience relapse, often attributed to the immunosuppressive nature tumor microenvironment. Emerging evidence suggests that other immune cell types, such as B and myeloid cells, play crucial roles in modulating efficacy cancer immunotherapies. However, there remains clear lack widely accessible immunological models capable accurately representing intricate...
Abstract CAR-T cell therapy is a revolutionary and highly effective treatment for hematologic malignancies, with rapid advancements particularly in applications to solid tumors. The development refinement of this innovative rely on efficient ex vivo assays evaluate cytotoxicity. Furthermore, advancement the tumors using requires specifically tumor models. Here, we report luciferase reporter cancer lines that endogenously express high levels key target antigens such as CD19, CD20, BCMA, or...
Glycol chitosan nanoparticles (CNPs) have emerged as an effective drug delivery system for cancer diagnosis and treatment. Although they great biocompatibility owing to biodegradable chemical structure low immunogenicity, sufficient information on in vivo toxicity understand the potential risks depending repeated high-dose not been adequately studied. Herein, we report results of evaluation CNPs focused number dose administration healthy mice provide a toxicological guideline better clinical...
Development of biopolymer-based imaging agents which can access rapidly and provide detailed information about the diseases has received much attention as an alternative to conventional agents. However, development nanomaterials for tumor still remains challenging due their low sensitivity image resolution. To surmount these limitations, multimodal have been developed, they were widely utilized theranostic applications. Herein, iodine containing echogenic glycol chitosan nanoparticles are...
Theranostic nanoparticles can deliver therapeutic agents as well diverse imaging to tumors. The enhanced permeation and retention (EPR) effect is regarded a crucial mechanism for the tumor-targeted delivery of nanoparticles. Although large number studies EPR theranostic have been performed, change in body size host on not fully understood. In this regard, comparative research needed behavior animals developing clinical stage. study, we prepared fluorophore (indocyanine green (ICG) or cyanine...
A common outcome of androgen deprivation in prostate cancer therapy is disease relapse and progression to castration-resistant (CRPC) via multiple mechanisms. To gain insight into the recent clinical findings that highlighted genomic alterations leading hyperactivation PI3K, we examined roles commonly expressed p110 catalytic isoforms PI3K a murine model Pten-null invasive CRPC. While blocking p110α had negligible effects development CRPC, either genetic or pharmacologic perturbation p110β...
Artocarpus altilis (Parkinson) Fosberg has traditionally been used in Indonesia for the treatment of liver cirrhosis, hypertension, and diabetes. In many other countries, it is malaria, yellow fever, dengue fever. It reported that A. extracts have antiatherosclerotic cytoprotective effects, but its molecular targets tumor cells are not yet fully understood. The partially purified fraction shown to inhibit STAT3 activity phosphorylation a dose-dependent manner. To identify active components,...
Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during chondrogenesis. The synthesized with a CB-cleavable peptide linked near infrared fluorescence (NIRF) dye and dark quencher, which can recover NIRF signal presence enzyme. activities two different sets are comparatively analyzed. higher activity show more extensive area chondrogenesis that directly reflects chondrogenic potency cells. expected provide...
Abstract Despite the considerable success of immune checkpoint therapies targeting T cells, a sizable proportion patients experience resistance or relapse due to immunosuppressive nature tumor microenvironment. Myeloid major component that suppresses effector lymphocytes, have emerged as an alternative and promising therapeutic target. However, there is deep lack widely accessible immunological models capable representing intricate three-way interaction between myeloid cells. To address this...
CAR-T cell-based therapies have demonstrated remarkable efficacy in treating malignant cancers, especially liquid tumors, and are increasingly being evaluated clinical trials for solid tumors. With the FDA’s initiative advancing alternative methods drug discovery development, full human ex vivo assays essential precision development. However, prevailing cell-mediated cytotoxicity limited by their use of radioactive materials, lack real-time measurement, low throughput, inability to automate,...
Recent studies highlight the integral role of interferon gamma receptor (IFNγR) pathway in T cell-mediated cytotoxicity against solid but not liquid tumors. IFNγ only directly facilitates tumor cell death by cells also indirectly promotes via myeloid phagocytosis microenvironment. Meanwhile, full human ex vivo immune checkpoint drug screening remains challenging. We hypothesized that an engineered activation site response element luciferase reporter (GAS-Luc2) can be utilized for diverse...
CAR-T cell-based therapies have demonstrated remarkable efficacy in treating malignant cancers, especially liquid tumors, and are increasingly being evaluated clinical trials for solid tumors. With the FDA’s initiative to advance alternative methods drug discovery development, full human ex vivo assays essential precision development. However, prevailing cell-mediated cytotoxicity limited by their use of radioactive materials, lack real-time measurement, low throughput, inability automate,...
<h3>Background</h3> Interferon-gamma (IFN-γ) is a cytokine that plays major role in activating cellular immunity and promoting anti-tumor immune response. Monitoring the level of IFN-γ has become industrial standard for evaluation efficacy immunotherapy drugs. However, widely used enzyme-linked immunosorbent assay (ELISA) not only costly time-consuming, but also often fails to detect low early-stage activation. Moreover, it difficult correctly quantify paracrine signaling secreted by T cells...