A5016588698- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- Neuroinflammation and Neurodegeneration Mechanisms
- Studies on Chitinases and Chitosanases
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Neuroscience and Neuropharmacology Research
- Viral Infections and Vectors
- HIV/AIDS Research and Interventions
- Infectious Encephalopathies and Encephalitis
- Adenosine and Purinergic Signaling
- Alzheimer's disease research and treatments
- Respiratory viral infections research
- Neurological Disease Mechanisms and Treatments
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Nuclear Receptors and Signaling
- Cancer-related Molecular Pathways
- Insect and Pesticide Research
- Tryptophan and brain disorders
- Parasites and Host Interactions
- Viral Infections and Immunology Research
Second Military Medical University
2024
Shanghai Changzheng Hospital
2024
Bethune International Peace Hospital
2023-2024
University of Pittsburgh
2010-2019
University of Pittsburgh Medical Center
2002-2008
Jiangsu University of Science and Technology
2005
Purdue University West Lafayette
1992-1998
University of Arizona
1991
CHI3L1 (YKL-40) is up-regulated in a variety of inflammatory conditions and cancers. We have previously reported elevated concentration the cerebrospinal fluid (CSF) human non-human primates with lentiviral encephalitis using immunohistochemistry showed that was associated astrocytes. In current study transcription expression were evaluated acute chronic neurological diseases. ELISA revealed significant elevation CSF multiple sclerosis (MS) patients as well mild aging. situ hybridization...
Three phenotypes of the antioxidant protein haptoglobin are known: Hp 1-1, 2-1 and 2-2.To investigate outcome HIV infection according to type.Haptoglobin were determined using starch gel electrophoresis in serum obtained from 653 HIV-infected Caucasians AIDS reference centers Gent (n = 184), Antwerp 309), Luxembourg 160). Survival was compared between types Kaplan-Meier curves. Plasma HIV-1 RNA quantified by reverse transcriptase PCR. Serum iron, transferrin saturation, ferritin, vitamin C...
Numerous inflammatory conditions are associated with elevated YKL-40 expression by infiltrating macrophages. Thus, we were surprised to observe minimal macrophage and abundant astrocyte of in neuroinflammatory conditions. The aims the current study better delineate this discrepancy, characterize factors that regulate macrophages astrocytes whether correlates cell morphology and/or activation state. In vitro, expressed high levels was induced classical inhibited alternative activation....
Chronic microglial activation is an important component of many neurological disorders, and imaging activated microglia in vivo will enable the detection improved treatment neuroinflammation. 1-(2-Chlorphenyl)-N-methyl-N-(1-methylpropyl)-3-isoquinoline-carbox-amide (PK11195), a peripheral benzodiazepine receptor ligand, has been used to image neuroinflammation, but extent which PK11195 binding distinguishes reactive astrocytes unclear. Moreover, may lack sufficient sensitivity for detecting...
Rare cases of West Nile virus (WNV)-associated inflammation outside the central nervous system (CNS) have been reported. We evaluated systemic distribution WNV in postmortem tissues during encephalitis six patients using immunohistochemistry. antigens were detected neurons CNS (all 6 cases), kidney (4 lungs (2 pancreas thyroid intestine stomach (1 case), esophagus bile duct skin prostate case) and testis case). In organs epithelial cells infected. none viral identified hepatocytes, heart,...
Abstract Activated microglia are an important feature of many neurological diseases and can be imaged in vivo using 1‐(2‐chlorophenyl)‐ N ‐methyl‐ ‐(1‐methylpropyl)‐3‐isoquinolinecarboxamide (PK11195), a ligand that binds the peripheral benzodiazepine receptor (PBR). ‐(2,5‐dimethoxybenzyl)‐ ‐(5‐fluoro‐2‐phenoxyphenyl) acetamide (DAA1106) is new PBR‐specific has been reported to bind PBR with higher affinity compared PK11195. We hypothesized this high‐affinity binding DAA1106 will enable...
YKL-40 (chitinase 3-like protein 1) is expressed in a broad spectrum of inflammatory conditions and cancers. We have previously reported that levels are elevated the cerebrospinal fluid (CSF) macaques humans with lentiviral encephalitis, as well multiple sclerosis (MS). The current study assessed temporal CSF subjects severe traumatic brain injury (TBI; Glasgow Coma Scale [GCS] score ≤8). also evaluated expression after parasagittal controlled cortical impact (CCI) over parietal cortex (2.8...
Human parechovirus 3 (HPeV3) is a picornavirus associated with neurologic disease in neonates. infection of preterm and term infants seizures destructive periventricular white matter lesions. Despite unremarkable cerebrospinal fluid (CSF), HPeV3 RNA can be amplified from CSF nasopharyngeal rectal swabs. We report pathologic findings 2 autopsy cases active infection. Both children were born approximately 1 month premature neurologically intact but, after few weeks, developed radiologic...
Background: Approximately one quarter of patients with AIDS develop severe cognitive deficits called HIV-associated dementia complex. There is some controversy regarding the importance viral load and distribution in mediating this neurologic disease. Objective: Brain HIV proviral RNA loads were compared to define molecular nature infection brain. Method: Neuropathologic examination was performed on brains from 10 autopsies that had short post-mortem intervals no evidence opportunistic...
We report a case of fatal fulminant West Nile virus (WNV) meningoencephalitis in an 87-year-old white male gardener. The Pennsylvania patient presented with 3-day history flu-like symptoms. His hospital course was gravely precipitous onset coma, ventilator dependence, loss cortical and brainstem functions within ten days admission. Acute serum cerebrospinal fluid samples revealed elevated levels WNV IgM antibodies by ELISA as well CSF blood cells, protein glucose. A complete autopsy...
Coxsackievirus B4 ( CB4 ) is a picornavirus associated with variety of human diseases, including neonatal meningoencephalitis, myocarditis and type 1 diabetes. We report the pathological findings in twin newborns who died during an acute infection. The twins were born month premature but well neurologically intact at birth. After week they developed lethal sepsis seizures. Histopathology demonstrated meningoencephalitis severe myocarditis, as pancreatitis, adrenal medullitis nephritis....
Cell cycle proteins regulate processes as diverse cell division and death. Recently their role in neuronal death has been reported several models of neurodegeneration. We have previously that two key regulators the cycle, retinoblastoma susceptibility gene product (pRb) transcription factor E2F1, exhibit altered immunostaining patterns simian immunodeficiency virus encephalitis (SIVE). Here we show E2F1 inactivated, hyperphosphorylated form pRb (ppRb) also human (HIVE). Quantification ppRb...
Human immunodeficiency virus encephalitis is characterized by infiltration of the brain with infected and activated macrophages; however, it not known why disease occurs after variable lengths infection in 25% immunosuppressed acquired immune deficiency syndrome patients. We determined vivo correlates (in peripheral blood central nervous system) for development progression lentiviral longitudinally following macrophages using positron emission tomography (PET). Using human postmortem tissues...
Traumatic brain injury ( TBI ) is accompanied by inflammatory infiltrates and CNS tissue response. The astrocytosis associated with has been proposed to have both beneficial detrimental effects on surviving neural tissue. We recently observed prominent astrocytic expression of YKL ‐40/chitinase 3‐like protein 1 CHI3L1 severity injury. physiological role in the unknown; however, its distribution at perimeter contusions temporal course suggested that it might be an important component response...
We used controlled cortical impact in mice to model human traumatic brain injury (TBI). Local was accompanied by distal diaschisis lesions that developed within regions anatomically connected the injured cortex. At 7 days after injury, histochemistry documented broadly distributed lesions, particularly contralateral cortex and ipsilateral thalamus striatum. Reactive astrocytosis microgliosis were noted multiple neural pathways also showed silver-stained cell processes bodies. Wisteria...
HIV infection in humans and simian immunodeficiency virus (SIV) macaques result encephalitis approximately one-quarter of infected individuals is characterized by infiltration the brain with activated macrophages. 1-(2-chlorphenyl)-N-methyl-N-(1-methylpropyl)-3-isoquinoline-carboxamide (PK11195) a ligand specific for peripheral benzodiazepine receptor abundant on macrophages expressed low levels noninfected brain. We hypothesized that positron-emission tomography (PET) carbon-11–labeled,...