A5063911988- CAR-T cell therapy research
- Vitamin D Research Studies
- Nutrition, Genetics, and Disease
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Cutaneous Melanoma Detection and Management
- CRISPR and Genetic Engineering
- Acute Lymphoblastic Leukemia research
- Melanoma and MAPK Pathways
- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Biotin and Related Studies
- Digestive system and related health
- Ethics in Clinical Research
- Education and Learning Interventions
- Christian Theology and Mission
- Diverse Topics in Contemporary Research
- Asthma and respiratory diseases
- Integrated Circuits and Semiconductor Failure Analysis
- Advancements in Semiconductor Devices and Circuit Design
- Eicosanoids and Hypertension Pharmacology
- melanin and skin pigmentation
- Genetic Mapping and Diversity in Plants and Animals
- Biosimilars and Bioanalytical Methods
Memorial Sloan Kettering Cancer Center
2011-2024
Kettering University
2024
Cellular Therapeutics (United Kingdom)
2019
University of Georgia
2016
National Institutes of Health
2012
National Cancer Institute
2012
Division of Cancer Epidemiology and Genetics
2012
National Institute of Environmental Health Sciences
2012
Cancer Research And Biostatistics
2011
Guidelines for follow‐up of patients with melanoma are based on limited evidence. To guide skin surveillance, we developed a risk prediction model subsequent primary melanomas, using demographic, phenotypical, histopathological, sun exposure and genomic factors. Using Cox regression frailty models, analysed data 2613 melanomas from 1266 recruited to the population‐based Genes, Environment Melanoma study in New South Wales, Australia, median 14 years' via cancer registry. Discrimination...
Abstract Multiplex immunoassay kits are essential for profiling immunomodulatory proteins, including cytokines, chemokines, and growth factors in humans animal models. Humanized mice, engineered to express human genes or incorporate cells, allow the study of immune responses vaccine efficacy. The ability distinguish mouse homologues same sample is valuable researchers. A novel multiplex was developed containing both mouse-specific human-specific antibody pairs, utilizing a rigorous screening...
Abstract Background: Observational and experimental studies suggest that vitamin D may influence breast cancer etiology. Most known effects of are mediated via the receptor (VDR). Few polymorphisms in VDR gene have been well studied relation to risk results inconsistent. Methods: We investigated haplotypes by genotyping 26 single nucleotide (SNP) (i) had known/suspected impact on function, (ii) were tagging SNPs for three haplotype blocks among whites, or (iii) previously associated with...
8515 Background: HDT-ASCT is the standard of care for patients with rel/ref diffuse large B-cell lymphoma (DLBCL). Herein, we report safety data on first 8 our phase I clinical trial 19-28z CAR-T post poor-risk aggressive B-NHL (NCT01840566). Methods: Eligibility this study includes chemosensitive to salvage therapy with: 1) FDG-PET (+) following 2 cycles or 2) bone marrow involvement B-NHL. T cells were retroviral transduced anti-CD19 scFV linked CD28 and CD3ζ signaling domains. Patients...
CD33 is a tractable target in acute myeloid leukemia (AML) for chimeric antigen receptor (CAR) T cell therapy, but clinical success lacking. We developed 3P14HLh28Z, novel CD33-directed CD28/CD3Z-based CAR derived from high-affinity binder obtained through membrane-proximal fragment immunization humanized mice. found that exclusively with the domain of necessary identification binders Compared clinically validated lintuzumab-based cells targeting distal epitopes, 3P14HLh28Z showed enhanced...
Background: Epidemiologic evidence suggests a negative relation between sunlight exposure and breast cancer risk. The hypothesized mechanism is sunlight-induced cutaneous synthesis of vitamin D.Objectives: Our goal was to examine sun its interaction with D receptor (VDR) gene variants on risk.Methods: We examined incidence among 31,021 private pesticide applicators’ wives, including 578 cases, enrolled in the prospective Agricultural Health Study cohort followed 8.6 years average. estimated...
Though genome-wide association studies (GWAS) have identified numerous susceptibility loci for common diseases, their use is limited due to the expense of genotyping large cohorts individuals. One potential solution ‘additional controls’, or genotype data from control individuals deposited in public repositories. While this approach has been used by several groups, genetically heterogeneous nature population United States makes potentially problematic. We empirically investigated utility a...
MDM2-SNP309 (rs2279744), a common genetic modifier of cancer incidence in Li-Fraumeni syndrome, modifies risk, age onset, or prognosis variety cancers. Melanoma and outcomes vary by sex, although SNP309 exerts an effect on the estrogen receptor, no consensus exists its melanoma. MDM2 MDM4 restrain p53-mediated tumor suppression, independently together. We investigated SNP309, priori MDM4-rs4245739, two coinherited variants, population-based cohort 3663 primary incident melanomas. Per-allele...
IntroductionOne of the main complications adoptive T cell therapy (ACT) is en-masse activation tumor-reactive cells leading to adverse events. These are classified as cytokine release syndrome (CRS) and CAR Related Encephalopathy Syndrome (CRES). Here, we report results analysis using a point care (POC) device predict immune-related toxicities in patients with relapsed/refractory (R/R) DLBCL treated axicabtagene ciloleucel (axi-cel).MethodsPatients R/R commercial axi-cel were included this...
Chimeric antigen receptor (CAR) T-cell therapy has resulted in remarkable clinical success the treatment of B-cell malignancies. However, its efficacy solid tumors is limited, primarily by target heterogeneity. To overcome heterogeneity, we developed CAR T cells that overexpress LIGHT, a ligand both lymphotoxin-β on cancer and herpes virus entry mediator immune cells. LIGHT-expressing displayed antigen-directed cytotoxicity mediated antigen-independent killing through interaction LIGHT with...
<p>(A) ELISA quantification of soluble LIGHT in cell culture media after 24 hours incubation. (B) Supernatant from LIGHT-CAR T cells and second-generation CAR were added to corresponding vitro cytolysis was assessed against AsPC1. Data is representative 2 independent experiments two different donors. (C) MIAPACA2. (D) Cytotoxicity assay with various mesothelin-directed constructs the addition recombinant 3 (E) Mesothelin-directed exhibited similar proliferation a repetitive antigen...
<p>(A) Violin plots showing quality metrics of single cells included in downstream analysis. nCount RNA: number RNA unique molecular identifiers (UMI); nFeature detected genes; nCount_ADT: antibody UMI; nFeature_ADT: antibodies; percent.mt: percentage mitochondrial gene expression; HTO_margin: difference between signals for the hashtag with highest signal and second signal. (B) (C) Weighted-nearest neighbor (WNN) UMAP colored by condition (CAR T cell construct timepoint) (top) CD4+ vs...
<p>(A) Flow cytometric analysis of LTβR expression MIAPACA2 cells after CRISPR knockout (KO). KO were also transduced with non-signaling without intracellular signaling portion (tLTBR). (B) healthy human donor-derived mesothelin-targeted CAR T cocultured tumor expressing GFP and firefly luciferase at different effector to ratios. Bioluminescence was measured 72 hours later plotted as a percentage the signal detected in coculture non-functional Meso-DEL-CAR cells. Plots represent 3...
<p>(A) CAR T cells infiltration and expression of LIGHT at the tumor site was quantified by flow cytometric analysis on day 14 post cell treatment. (B) (C) Quantification per gram AsPC1 mass Plot is representative 4 to 5 mice treatment group. Immunohistochemistry staining mesothelin-negative cancer line, Panc1. Negative control. (D) mesothelin-positive MDA-MB-231. Positive (E) various PDX slides samples validate their mesothelin expression. (F) Flow in PDAC2, one selected for vivo...