A5016802895- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Fault Detection and Control Systems
- Antimicrobial Peptides and Activities
- Plant Virus Research Studies
- Polydiacetylene-based materials and applications
- Neonatal Respiratory Health Research
- RNA regulation and disease
- Inflammasome and immune disorders
- Extracellular vesicles in disease
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell death mechanisms and regulation
Xinqiao Hospital
2022-2024
Army Medical University
2022-2023
Chinese People's Liberation Army
2023
Acute lung injury (ALI) is characterized by severe inflammation and damage to the air-blood barrier, resulting in respiratory function life-threatening outcomes. Macrophage polarization plays an essential role occurrence, development, outcome of ALI. As drug carriers, self-assembled DNA nanostructures can potentially overcome drawbacks limitations traditional anti-inflammatory agents owing their nontoxicity, programmability, excellent structural control at nanoscale. A small interfering RNA...
Target double-stranded DNA (dsDNA) or single-stranded (ssDNA) can activate the trans-cleavage activity of CRISPR/Cas12a, cutting surrounding non-target ssDNA arbitrarily. In a typical CRISPR/Cas12a system, this ssDNA, with fluorescent tag and its quencher incorporated at both ends (ssDNA-FQ), is usually used as reporter. Here, 2-aminopurine probe (T-pro 4), made by inserting four 2-APs in was screened for using reporter system. Compared ssDNA-FQ, each 2-AP cleaved activated multi-unit...
RNA imaging in live cells can provide comprehensive information on the expression, localization, degradation, storage, and regulation of cells, which is crucial for basic biology clinical research. Our previous research finds that slicing facilitated crRNA typical CRISPR/Cas12a system at a fitted site did not affect its trans-cleavage activity, was previously reported to be triggered by targeted ssDNA or dsDNA, mini crRNA-mediated (MCM-CRISPR/Cas12a) proposed. Here, we further apply it...
Acute lung injury (ALI) and acute respiratory distress syndrome are triggered by complicated molecular mechanisms, which the prime causes of high mortality in critical patients. Recent research suggests that release levels IL-1β IL-18, resulting from pyroptosis, plays a pivotal role causing cytokine storms lead to severe damage. DNA nanostructures possess desirable properties, such as biocompatibility, programmability, biodegradability, nontoxicity, making them suitable for use drug delivery...
Emerging CRISPR/Cas12a system is receiving increasing attention in the field of biological analysis, especially nucleic acid testing. However, due to need for recognize single-stranded or double-stranded DNA under mediation crRNA activate its trans-cleavage activity, direct detection RNA limited. Here, we prospectively find that slicing facilitated typical at a fitted site did not affect and mini crRNA-mediated (MCM-CRISPR/Cas12a) was proposed based on this. To expand application this...
Some non-coding RNAs are abnormally expressed during the occurrence and development of diseases, so it is necessary to develop analytical methods that can specifically sensitively detect them. In typical CRISPR/Cas12a system, a complete crRNA recognize single-stranded or double-stranded DNA activate its trans-cleavage activity, which limits application in RNA detection. Here, we prospectively find slicing facilitated system at fitted site did not affect mini crRNA-mediated...
Anti-Inflammatory Therapy In article number 2200008 by Mingdong Hu, Hang Qian, and co-workers, a dual therapy DNA nanoplatform consisting of spermidine mTOR siRNA is constructed synergistic anti-inflammatory effect demonstrated in vitro an acute lung injury mouse model.