A5068591369- Lipid Membrane Structure and Behavior
- Spectroscopy and Quantum Chemical Studies
- Metabolomics and Mass Spectrometry Studies
- Sphingolipid Metabolism and Signaling
- Nanopore and Nanochannel Transport Studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Ion channel regulation and function
- Electrostatics and Colloid Interactions
- Neuroscience and Neuropharmacology Research
- Force Microscopy Techniques and Applications
- Radiation Therapy and Dosimetry
- Erythrocyte Function and Pathophysiology
- Cardiomyopathy and Myosin Studies
- Peroxisome Proliferator-Activated Receptors
- Fatty Acid Research and Health
- Quantum, superfluid, helium dynamics
- Muscle Physiology and Disorders
- Protein Structure and Dynamics
- Surfactants and Colloidal Systems
- Research in Social Sciences
- Advanced Radiotherapy Techniques
- Advanced Proteomics Techniques and Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Protein Interaction Studies and Fluorescence Analysis
- Spectroscopy Techniques in Biomedical and Chemical Research
Varian Medical Systems (United States)
2015-2024
VTT Technical Research Centre of Finland
2009-2011
Aalto University
2010
University of Southern Denmark
2010
Stockholm University
2010
Tampere University
2010
Inserm
2010
Tieto (Finland)
2009
Helsinki Institute of Physics
2004-2008
University of Oulu
2003
Identification of early mechanisms that may lead from obesity towards complications such as metabolic syndrome is great interest. Here we performed lipidomic analyses adipose tissue in twin pairs discordant for but still metabolically compensated. In parallel studied more evolved states by investigating a separated set individuals considered to be morbidly obese. Despite lower dietary polyunsaturated fatty acid intake, the obese had increased proportions palmitoleic and arachidonic acids...
The paradigm of biological membranes has recently gone through a major update. Instead being fluid and homogeneous, recent studies suggest that are characterized by transient domains with varying fluidity. In particular, number experimental have revealed the existence highly ordered lateral rich in sphingomyelin cholesterol (CHOL). These domains, called functional lipid rafts, been suggested to take part variety dynamic cellular processes such as membrane trafficking, signal transduction,...
We describe how membrane proteins diffuse laterally in the plane together with lipids surrounding them. find a number of intriguing phenomena. The lateral displacements protein and are strongly correlated, as neighboring form dynamical protein−lipid complex, consisting ∼50−100 lipids. diffusion complex is much slower compared to rest also strong directional correlation between movements its vicinity. results imply that crowded environments there no "free" lipids, they all influenced by...
A low level of high density lipoprotein cholesterol (HDL-C) is a powerful risk factor for cardiovascular disease. However, despite the reported key role apolipo-proteins, specifically, apoA-I, in HDL metabolism, lipid molecular composition particles subjects with and HDL-C levels currently unknown. Here lipidomics was used to study derived from well-characterized subjects. Low had elevated triacylglycerols diminished lysophosphatidylcholines sphingomyelins. Using information about these two...
Using extensive atomistic simulations, we show that there is a single experimentally accessible parameterthe sterol tiltthat can be used to determine sterol's capability induce order, and thus promote, e.g., formation of lipid rafts. The observations also facilitate designing new effective sterols.
Structure and dynamics of voltage-gated ion channels, in particular the motion S4 helix, is a highly interesting hotly debated topic current membrane protein research. It has critical implications for insertion stabilization proteins as well finding how transitions occur proteins-not to mention numerous applications drug design. Here, we present full 1 micros atomic-detail molecular simulation an integral Kv1.2 channel, comprising 120,000 atoms. By applying 0.052 V/nm hyperpolarization,...
We study the structure and dynamics of spherical high density lipoprotein (HDL) particles through coarse-grained multi-microsecond molecular simulations. simulate both a lipid droplet without apolipoprotein A-I (apoA-I) full HDL particle including two apoA-I molecules surrounding compartment. The present models are first ones among computational studies where size composition realistic, corresponding to human serum HDL. focus on role lipids in dynamics. Particular attention is paid assembly...
Currently, there is no comprehensive model for the dynamics of cellular membranes. The understanding even basic dynamic processes, such as lateral diffusion lipids, still quite limited. Recent studies one-component membrane systems have shown that instead single-particle motions, driven by a more complex, concerted mechanism lipid (E. Falck et al., J. Am. Chem. Soc., 2008, 130, 44-45), where and its neighbors move in unison terms loosely defined clusters. In this work, we extend previous...
Free volume pockets or voids are crucial for a variety of dynamic processes in lipid membranes. Voids facilitate the diffusion molecules plane membrane and highly relevant permeation small solutes across membrane. We employ atomic-scale molecular dynamics simulations to study free packing properties different systems, focusing on lipids commonly found rafts. find that membranes comprised saturated (DMPC, DPPC) diunsaturated (PLPC) phosphatidylcholine (PC) have many common features, while...
Purpose: To develop a new robust optimization strategy for intensity‐modulated proton therapy as an important step in translating treatment planning from research to clinical applications. Methods: In selective optimization, worst‐case‐based algorithm is extended, and terms of the objective function are selectively computed either worst‐case dose or nominal dose. Two lung cancer cases one head neck case were used demonstrate practical significance proposed strategy. The had minimal tumor...
Calcineurin, a Ca 2+ –calmodulin‐dependent protein phosphatase (PP2B) is one of the links between signals and regulation gene transcription in cardiac muscle. We studied signal specificity calcineurin activation experimentally with modelling. In rat atrial preparation, an increase pacing frequency increased nuclear activity calcineurin‐sensitive factor, factor activated T‐cells (NFAT), 2‐fold cyclosporin A (CsA)‐sensitive manner. line this, modelling results predicted that transients encodes...
Activation of the contractile machinery in skeletal muscle is initiated by action-potential-induced release Ca2+ from sarcoplasmic reticulum (SR). Several proteins involved SR are affected calmodulin kinase II (CaMKII)-induced phosphorylation vitro, but effect intact cell remains uncertain and focus present study. CaMKII inhibitory peptide or inactive control was injected into single isolated fast-twitch fibres mouse flexor digitorum brevis muscles, on free myoplasmic [Ca2+] ([Ca2+]i) force...
Activation of the contractile machinery in skeletal muscle is initiated by action-potential-induced release Ca2+ from sarcoplasmic reticulum (SR). Several proteins involved SR are affected calmodulin kinase II (CaMKII)-induced phosphorylation vitro, but effect intact cell remains uncertain and focus present study. CaMKII inhibitory peptide or inactive control was injected into single isolated fast-twitch fibres mouse flexor digitorum brevis muscles, on free myoplasmic [Ca2+] ([Ca2+]i) force...
In Intensity Modulated Proton Therapy (IMPT), the weights of individual pencil-beams or spots are optimized to fulfil dosimetric constraints. Theses usually located on a regular lattice and their positions fixed during optimization. many cases, range spot may however be limited, leading sometimes sub-optimal plan quality. An emblematic use case is delivery at ultra-high dose rate (FLASH-RT), for which typically constrained high values.