A5009293856- Metabolomics and Mass Spectrometry Studies
- Cancer, Lipids, and Metabolism
- Fatty Acid Research and Health
- Biofuel production and bioconversion
- Peroxisome Proliferator-Activated Receptors
- Plant biochemistry and biosynthesis
- Liver Disease Diagnosis and Treatment
- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Lipid metabolism and biosynthesis
- Algal biology and biofuel production
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Analytical Chemistry and Chromatography
- Diet, Metabolism, and Disease
- Cancer, Hypoxia, and Metabolism
- Natural product bioactivities and synthesis
- Aquaculture Nutrition and Growth
- Mass Spectrometry Techniques and Applications
- Nutritional Studies and Diet
- Advanced Cellulose Research Studies
- Transgenic Plants and Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes, Cardiovascular Risks, and Lipoproteins
VTT Technical Research Centre of Finland
2014-2024
Uppsala University
2019-2022
Tieto (Finland)
2016-2021
Steno Diabetes Centers
2014
BG Klinikum Hamburg
2011
University of California, Davis
2011
University of Cambridge
2011
German Breast group
2011
Bioengineering (Switzerland)
2010-2011
Abstract Activation of lipid metabolism is an early event in carcinogenesis and a central hallmark many cancers. However, the precise molecular composition lipids tumors remains generally poorly characterized. The aim present study was to analyze global profiles breast cancer, integrate results protein expression, validate findings by functional experiments. Comprehensive lipidomics conducted 267 human tissues using ultraperformance liquid chromatography/ mass spectrometry. products de novo...
Identification of early mechanisms that may lead from obesity towards complications such as metabolic syndrome is great interest. Here we performed lipidomic analyses adipose tissue in twin pairs discordant for but still metabolically compensated. In parallel studied more evolved states by investigating a separated set individuals considered to be morbidly obese. Despite lower dietary polyunsaturated fatty acid intake, the obese had increased proportions palmitoleic and arachidonic acids...
Previous studies show that children who later progress to type 1 diabetes (T1D) have decreased preautoimmune concentrations of multiple phospholipids as compared with nonprogressors. It is still unclear whether these changes associate development β-cell autoimmunity or specifically clinical T1D. Here, we studied umbilical cord serum lipidome in infants developed T1D (N = 33); three four 31) islet autoantibodies, two one 48) autoantibody during the follow-up; and controls 143) matched for...
The gut microbiota influences many aspects of host metabolism. We have previously shown that the presence a remodels lipid composition. Here we investigated how interaction between and dietary lipids regulates composition in liver plasma, gene expression liver. Germ-free conventionally raised mice were fed lard or fish oil diet for 11 weeks. performed lipidomics analysis serum microarray As expected, most variation dataset was induced by diet, abundance classes differed oil. However, also...
Plant cell cultures from cloudberry, lingonberry and stoneberry were studied in terms of their nutritional properties as food. Carbohydrate, lipid protein composition, vitro digestibility sensory investigated. Dietary fibre content varied between 21.2 36.7%, starch 0.3 1.3% free sugar 17.6 33.6%. Glucose fructose the most abundant sugars. High contents 13.7 18.9% recorded all samples had a balanced amino acid profile. In digestion assay showed hydrolysis by digestive enzymes fresh cells but...
Adipose tissue dysfunction is an important determinant of obesity-associated, lipid-induced metabolic complications. Ceramides are well-known mediators insulin resistance in peripheral organs such as muscle. DEGS1 the desaturase catalyzing last step main ceramide biosynthetic pathway. Functional suppression activity results substantial changes species likely to affect fundamental biological functions oxidative stress, cell survival, and proliferation. Here, we show that degs1 expression...
The sustainability of the global protein production is seriously challenged, why upgrading from new or under-utilized sources such as brewer's spent grain (BSG) desirable. In this article a method for high-yield extraction BSG with deep eutectic solvents (DESs) reported. Novel carboxylate salt - urea DESs are described and using 6 these mixtures compared. 90 wt% NaAcO : (molar ratio 1:2) was compared to choline chloride (1:2), which first found be more suitable extractant up 79% yields BSG....
Sustainability and safety aspects of plant cell cultures as food are presented. Applicability dairy side streams carbon source use natural growth enhancers in cultivation shown. Biotechnologically produced cellular products currently emerging to replace add into the portfolio agriculturally derived commodities. Plant used for could supplement current production. However, still many need be resolved before this new concept can enter market. Issues related sustainability human consumption...
In a recent FIELD study the fenofibrate therapy surprisingly failed to achieve significant benefit over placebo in primary endpoint of coronary heart disease events. Increased levels atherogenic homocysteine were observed some patients assigned but molecular mechanisms behind this are poorly understood. Herein we investigated HDL lipidomic profiles associated with treatment and drug-induced Hcy substudy. We found that leads complex compositional changes including increased apoA-II,...
The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is a widely used industrial host organism for protein production. In cultivations, it can produce over 100 g/l of extracellular protein, mostly constituting cellulases and hemicellulases. order to improve production T. the transcriptional regulation secretory pathway factors have been extensively studied. However, metabolism under conditions has not received much attention. To understand physiology we carried out...
Trichoderma reesei is one of the main sources biomass-hydrolyzing enzymes for biotechnology industry. There a need improving its enzyme production efficiency. The use metabolic modeling simulation and prediction this organism’s metabolism potentially valuable tool capabilities. An accurate model needed to perform analysis. A whole-genome T. has been reconstructed together with models 55 related species using reconstruction algorithm CoReCo. previously published CoReCo method improved obtain...
Biocolourants have been investigated as alternatives to synthetic dyes. However, natural origin per se is not a label of harmlessness and research needed obtain safe We studied the cytotoxicity extracts from fungal (
Consumption of fresh and minimally processed foods such as seeds a part healthy diet is trend. Unfortunately, fat-rich are often contaminated with pathogenic microorganisms face frequent product recalls. Electron beams have been applied microbial decontamination measure for decades. Conventionally high energy electron (HEEB) being used, whereas low (LEEB, <300 keV) only recently introduced to the food industry more studies needed. beam treatment has several advantages over other...
Abstract Lytic polysaccharide monooxygenases (EC1.14.99.53-56, LPMOs) are oxidative enzymes with the capability to enhance lignocellulose saccharification as well nanofibrillation of cellulosic fibres. The parameters affecting efficiency modification cotton linters and softwood kraft fibres by LPMO from Trichoderma reesei ( Tr AA9A) were studied here. Circular dichroism experiments carried out analyze thermal stability indicate optimum temperature for experiments. enzyme was found be most...
The contribution of peroxisomal proliferator-activated receptor (PPAR)-γ agonism in pancreatic β-cells to the antidiabetic actions thiazolidinediones has not been clearly elucidated. Genetic models β-cell PPARγ ablation have revealed a potential role for expansion obesity but limited normal physiology. Here we overexpressed PPARγ1 or PPARγ2 specifically mice subjected high-fat feeding using an associated adenovirus (β-PPARγ1-HFD and β-PPARγ2-HFD mice). We show β-cell-specific overexpression...
High lipid heterotrophic microalgae (HM) Schizochytrium limacinum is a good dietary source of long‐chained polyunsaturated fatty acids. HM biomass has successfully been used in aquafeeds. However, the high saturated acid content triacylglycerol (TAG) could limit applications as main feed source. Enzymatic interesterification oil with unsaturated oils may increase utilization efficiency and remove technical challenges using such oils. In present study, extracted from (Alltech Inc.) rapeseed...
Cellulose oxidation and enzymatic treatments can be employed to reduce the energy consumption in production of cellulose nanofibrils (CNFs). Lytic polysaccharide monooxygenases (LPMOs) offer new biocatalytic tools for oxidative pretreatment cellulosic fibres this process. In work capability LPMO enzymes enhance fibrillation bleached softwood kraft was studied using two enzymes, i.e. C1/C4-oxidising AA9A from Trichoderma reesei (Tr AA9A) C1-oxidising AA9E Podospora anserina (Pa AA9E). The...