A5090536075- Gut microbiota and health
- Diet and metabolism studies
- Clostridium difficile and Clostridium perfringens research
- Adipokines, Inflammation, and Metabolic Diseases
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Dietary Effects on Health
- Metabolomics and Mass Spectrometry Studies
- Tryptophan and brain disorders
- Fungal and yeast genetics research
- Probiotics and Fermented Foods
- Immune Response and Inflammation
- Diet, Metabolism, and Disease
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- Signaling Pathways in Disease
- Genetic Syndromes and Imprinting
- Retinoids in leukemia and cellular processes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Drug Transport and Resistance Mechanisms
- Lipid metabolism and biosynthesis
- Regulation of Appetite and Obesity
- Immunodeficiency and Autoimmune Disorders
- Peroxisome Proliferator-Activated Receptors
University of Gothenburg
2016-2025
Sahlgrenska University Hospital
2014-2018
Wallenberg Wood Science Center
2017
University of Oslo
2008-2010
Highlights•The gut microbiota contributes to phenotypic differences in mice fed lard or fish oil•Mice lacking MyD88 TRIF are protected against WAT inflammation•Microbial-derived factors induce CCL2 adipocytes through TLR4, MyD88, and TRIF•Microbial-induced enhances macrophage accumulation WATSummaryDietary lipids may influence the abundance of circulating inflammatory microbial factors. Hence, inflammation white adipose tissue (WAT) induced by dietary be partly dependent on their interaction...
Objective The gut microbiota has been implicated as an environmental factor that modulates obesity, and recent evidence suggests microbiota-mediated changes in bile acid profiles signalling through the nuclear receptor farnesoid X (FXR) contribute to impaired host metabolism. Here we investigated if obesity associated phenotypes FXR. Design We fed germ-free (GF) conventionally raised (CONV-R) wild-type Fxr−/− mice a high-fat diet (HFD) for 10 weeks. monitored weight gain glucose metabolism...
Individuals with type 2 diabetes have aberrant intestinal microbiota. However, recent studies suggest that metformin alters the composition and functional potential of gut microbiota, thereby interfering diabetes-related microbial signatures. We tested whether specific microbiota profiles are associated prediabetes (defined as fasting plasma glucose 6.1–7.0 mmol/l or HbA1c 42–48 mmol/mol [6.0–6.5%]) a range clinical biomarkers poor metabolic health. In present case–control study, we analysed...
<h3>Background</h3> Obesity is associated with accumulation of macrophages in white adipose tissue (WAT), which contribute to the development insulin resistance. Germ-free (GF) mice have reduced adiposity and are protected against diet-induced obesity, <h3>Objective</h3> To investigate whether gut microbiota and, specifically, gut-derived lipopolysaccharide (LPS) promote WAT inflammation impaired glucose metabolism. <h3>Method</h3> Macrophage composition expression proinflammatory...
Abstract Obesity is associated with a cluster of metabolic disorders, low-grade inflammation and altered gut microbiota. Whether host metabolism controlled by intestinal innate immune system the microbiota unknown. Here we report that inducible epithelial cell-specific deletion MyD88 partially protects against diet-induced obesity, diabetes inflammation. This increased energy expenditure, an improved glucose homeostasis, reduced hepatic steatosis, fat mass Protection transferred following...
Abstract Previous microbiome and metabolome analyses exploring non-communicable diseases have paid scant attention to major confounders of study outcomes, such as common, pre-morbid co-morbid conditions, or polypharmacy. Here, in the context ischemic heart disease (IHD), we used a design that recapitulates initiation, escalation response treatment over time, mirroring longitudinal would otherwise be difficult perform given protracted nature IHD pathogenesis. We recruited 1,241 middle-aged...
The human gut microbiota has gained interest as an environmental factor that may contribute to health or disease
Dietary lipids can affect metabolic health through gut microbiota-mediated mechanisms, but the influence of lipid-microbiota interaction on liver steatosis is largely unknown. We investigate impact dietary human microbiota composition and effects microbiota-lipid interactions in male mice. In humans, low intake saturated fatty acids (SFA) associated with increased microbial diversity independent fiber intake. mice, poorly absorbed long-chain SFA, particularly stearic acid, induce a shift...
Objective- To investigate the effect of gut microbiota and diet on atherogenesis. Approach Results- Here, we investigated interaction between atherosclerosis by feeding germ-free or conventionally raised Apoe
Depot-dependent differences in adipose tissue physiology may reflect specialized functions and local interactions between adipocytes surrounding tissues. We combined time-resolved microarray analyses of mesenteric- (MWAT), subcutaneous- (SWAT) epididymal (EWAT) during high-fat feeding male transgenic ApoE3Leiden mice with histology, targeted lipidomics biochemical metabolic pathways to identify differentially regulated processes site-specific functions. EWAT was found exhibit physiological...
The gut microbiota influences many aspects of host metabolism. We have previously shown that the presence a remodels lipid composition. Here we investigated how interaction between and dietary lipids regulates composition in liver plasma, gene expression liver. Germ-free conventionally raised mice were fed lard or fish oil diet for 11 weeks. performed lipidomics analysis serum microarray As expected, most variation dataset was induced by diet, abundance classes differed oil. However, also...
The gut microbiota has been implicated in the aetiology of obesity and associated comorbidities. Patients with Prader-Willi syndrome (PWS) are obese but partly protected against insulin resistance. We hypothesised that PWS patients differs from non-genetically controls correlate to metabolic health. Therefore, here we used as a model study role prevention complications linked obesity.
The Saccharomyces cerevisiae N-terminal acetyltransferase NatB consists of the subunits Nat3p and Mdm20p. We found by two-dimensional PAGE analysis that nat3Delta exhibited protein expression during growth in basal medium resembling salt-adapted wild-type cells. stress-induced carboxypeptidase Y (CPY) inhibitor phosphatidylethanolamine-binding family member Tfs1p was identified as a novel substrate. acetylation status Tfs1p, Act1p, Rnr4p both wild type confirmed tandem mass spectrometry....
Sucrose-rich diets promote hepatic de novo lipogenesis (DNL) and steatosis through interactions with the gut microbiota. However, role of sugar-microbiota dynamics in absence dietary fat remains unclear. This study aimed to investigate effects a high-sucrose, zero-fat diet (ZFD) on host metabolism conventionally raised (CONVR) germ-free (GF) mice. CONVR GF mice were fed ZFD, lipid accumulation, gene expression, metabolite levels analyzed. DNL activity was assessed by measuring malonyl-CoA...
Gut microbiota modulates adiposity and glucose metabolism in humans mice. Here we investigated how colonization of germ-free (GF) mice affects kinetics metabolism. Adiposity were evaluated at different time points ex-GF antibiotic treated after with gut from a conventionally raised (CONV-R) mouse. Mouse physiology, microbiome configuration, serum cytokine levels, gene expression for inflammatory markers performed tissues. Colonization resulted bi-phasic impairment: the first phase occurring...
ABSTRACT The N-terminal acetyltransferase NatB in Saccharomyces cerevisiae consists of the catalytic subunit Nat3p and associated Mdm20p. We here extend our present knowledge about physiological role by a combined proteomics phenomics approach. found that strains deleted for either NAT3 or MDM20 displayed different growth rates morphologies specific stress conditions, demonstrating two subunits have partly individual functions. Earlier reported phenotypes nat3Δ strain been with altered...
Loss of appetite is a hallmark inflammatory diseases. The underlying mechanisms remain undefined, but it known that myeloid differentiation primary response gene 88 (MyD88), an adaptor protein critical for Toll-like and IL-1 receptor family signaling, involved. Here we addressed the question determining in which cells MyD88 signaling results anorexia development occurs by using chimeric mice animals with cell-specific deletions. We found MyD88-knockout mice, are resistant to bacterial...
Gut-derived inflammatory factors can impair glucose homeostasis, but the underlying mechanisms are not fully understood. In this study, we investigated how hepatic gene expression is regulated by gut colonization status through myeloid differentiation primary response 88 (MYD88) and one of genes, lipopolysaccharide-binding protein (Lbp), affects insulin signaling systemic homeostasis.Liver transcriptomics analysis was conducted on four groups mice fed a chow diet: conventionally raised...
Mice with deletion of Cyp2c70 have a human-like bile acid composition, display age- and sex-dependent signs hepatobiliary disease can be used as model to study interactions between acids the gut microbiota in cholestatic liver disease. In present study, we rederived Cyp2c70-/- mice germ-free (GF) colonized them human or mouse investigate whether presence protective cholangiopathic associated Cyp2c70-deficiency. GF showed reduced neonatal survival, fibrosis, distinct cholangiocyte...