A5018963957- Cardiovascular Disease and Adiposity
- Aortic Disease and Treatment Approaches
- Microbial metabolism and enzyme function
- Birth, Development, and Health
- Metabolism, Diabetes, and Cancer
- Receptor Mechanisms and Signaling
- Fatty Acid Research and Health
- Aortic aneurysm repair treatments
- Atherosclerosis and Cardiovascular Diseases
- Renal and related cancers
- Cardiovascular Function and Risk Factors
- Pancreatic function and diabetes
- Congenital heart defects research
University of Kentucky
2017-2025
Cardiovascular Research Center
2023
β-aminopropionitrile (BAPN) is a pharmacological inhibitor of LOX (lysyl oxidase) and LOXLs (LOX-like proteins). Administration BAPN promotes aortopathies, although there paucity data on experimental conditions to generate pathology. The objective this study was define parameters determine whether equivalent or variable aortopathies were generated throughout the aortic tree during administration in mice.
Thoracic aortopathy is influenced by angiotensin II (AngII) and exhibits regional heterogeneity with the proximal region of thoracic aorta being susceptible. Smooth muscle cells (SMCs) selected fibroblasts in this are derived from two embryonic origins: second heart field (SHF) cardiac neural crest (CNC). While our previous study revealed a critical role SHF-derived AngII-mediated formation, contribution CNC-derived remains unclear. Mef2c-Cre R26R mT/mG mice were infused AngII (1,000...
Background: Plasminogen activator inhibitor-1 (PAI-1) is highly abundant in human thoracic aortic aneurysms (TAA). This high abundance mimicked TAAs created by chronic infusion of angiotensin II (AngII) into mice. The purpose this study was to determine whether deletion PAI-1 influenced the development during AngII infusion. Methods and Results: To role acute phases AngII-induced pathology, whole-body deficient mice (PAI-1 -/-) wild type littermates +/+) were infused with (1,000 ng/kg/min)...
Background and Objective: Commonly used quantification methods of atherosclerosis in mouse models include en face analysis lesions the aorta lesion area measurements aortic roots using cross-sections. The major shortcoming these is that they quantify surface area, which does not reflect total volume atherosclerosis. purpose this study to develop a method measure volume. Methods Results: Male LDL receptor -/- mice were fed Western diet for 12 weeks, then euthanized collect roots. Serial...
ABSTRACT BACKGROUND β-aminopropionitrile (BAPN) is a pharmacological inhibitor of lysyl oxidase and oxidase-like proteins. Administration BAPN promotes aortopathies, although there paucity data on experimental conditions to generate pathology. The objective this study was define parameters determine whether equivalent or variable aortopathies were generated throughout the aortic tree during administration in mice. METHODS administered drinking water for period ranging from 1 12 weeks....
The aim of the study was to assess effect insulin receptor (IR) signaling on hepatic apoA-I metabolism. experimental approach compare expression, secretion and cellular localization in IRfl/fl mice LIRKO which receptors were specifically deleted by AAV delivered Cre-recombinase. Results showed that IR mRNA protein levels markedly reduced livers compared control mice. As expected, exhibited decreased glucose tolerance signaling. Knockdown plasma HDL cholesterol levels. Whereas similar...