A5022368482- Aortic Disease and Treatment Approaches
- Aortic aneurysm repair treatments
- Connective tissue disorders research
- Macrophage Migration Inhibitory Factor
- Cardiac and Coronary Surgery Techniques
- Renin-Angiotensin System Studies
- Cardiac Valve Diseases and Treatments
- Infectious Aortic and Vascular Conditions
- Congenital heart defects research
- Atherosclerosis and Cardiovascular Diseases
- Receptor Mechanisms and Signaling
- Immune cells in cancer
- Hormonal Regulation and Hypertension
- Inflammatory mediators and NSAID effects
- Medical Imaging and Pathology Studies
- Cardiac Fibrosis and Remodeling
- Renal Diseases and Glomerulopathies
- Cerebrovascular and genetic disorders
- MicroRNA in disease regulation
- Antiplatelet Therapy and Cardiovascular Diseases
- Immune Response and Inflammation
- Cardiovascular Disease and Adiposity
- Renal and related cancers
- Advanced Glycation End Products research
- Lung Cancer Diagnosis and Treatment
University of Kentucky
2021-2025
Kurume University
1988-2023
Cardiovascular Research Center
2022
Kitasato University
2008-2018
Cardiovascular Institute Hospital
2014
Palo Alto University
2010
Kurume University Medical Center
1989
β-aminopropionitrile (BAPN) is a pharmacological inhibitor of LOX (lysyl oxidase) and LOXLs (LOX-like proteins). Administration BAPN promotes aortopathies, although there paucity data on experimental conditions to generate pathology. The objective this study was define parameters determine whether equivalent or variable aortopathies were generated throughout the aortic tree during administration in mice.
Abdominal aortic aneurysm (AAA) is a potentially life-threatening disease that common in older individuals. Currently, therapeutic options are limited to surgical interventions. Although it has long been known AAA tissue enriched B cells and immunoglobulins, their involvement pathogenesis remains controversial.We investigated the role of immunoglobulins murine model AAA, induced with periaortic application CaCl2, human AAA. Both mouse showed B-cell infiltration. Mouse deposition IgG...
Thoracic aortopathy is influenced by angiotensin II (AngII) and exhibits regional heterogeneity with the proximal region of thoracic aorta being susceptible. Smooth muscle cells (SMCs) selected fibroblasts in this are derived from two embryonic origins: second heart field (SHF) cardiac neural crest (CNC). While our previous study revealed a critical role SHF-derived AngII-mediated formation, contribution CNC-derived remains unclear. Mef2c-Cre R26R mT/mG mice were infused AngII (1,000...
ABSTRACT Thoracic aortopathies are life-threatening diseases including aneurysm, dissection, and rupture. Phosphodiesterases (PDEs) regulate intracellular cyclic nucleotide concentrations. Recent studies report the influences of cilostazol, a PDE3 inhibitor, sildenafil, PDE5 on abdominal aortic aneurysm formation. However, their impacts thoracic aortopathy remain unknown. In this study, we investigated whether cilostazol sildenafil affect induced by β-aminopropionitrile (BAPN) administration...
Acute aortic dissection (AAD) is caused by the disruption of intimomedial layer walls, which immediately life-threatening. Although recent studies indicate importance proinflammatory response in pathogenesis AAD, mechanism to keep destructive inflammatory check unknown. Here, we report that induction tenascin-C (TNC) a stress-evoked protective against acute hemodynamic and humoral stress aorta. Periaortic application CaCl2 stiffening abdominal aorta, augmented TNC suprarenal aorta...
Although the pathogenesis of abdominal aortic aneurysm (AAA) remains unclear, evidence is accumulating to support a central role for inflammation. Inflammatory responses are coordinated by various soluble cytokines which IL-6 one major proinflammatory cytokines. In this study we examined in experimental AAA induced periaortic exposure CaCl2 mice. We now report that administration MR16-1, neutralizing monoclonal antibody specific mouse receptor, mildly suppressed development AAA. The...
Background Aortic dissection ( AD ) is a life‐threatening medical emergency caused by the abrupt destruction of intimomedial layer aortic walls. Given that previous studies have reported involvement proinflammatory cytokine interleukin‐6 in pathogenesis, we investigated role signal transduction and activator transcription 3 signaling, downstream pathway macrophages pathogenesis . Methods Results We characterized pathological molecular events triggered stress, which can lead to stress on...
Aortic dissection (AD) is a fatal disease that occurs suddenly without preceding clinical signs or symptoms. Although high salt intake proposed risk factor for cardiovascular diseases, the relationship between AD and has not been clarified. We examined effect of high-salt challenge on mouse model. Approach Results: was induced in male mice by continuous infusion β-aminopropionitrile Ang II (angiotensin II). High-salt exacerbated aortic wall destruction AD. Deletion Il17a (IL-17KO [IL...
TNF-α is a critical mediator of hepatic microcirculatory dysfunction during endotoxemia. The present study was to investigate the role thromboxane A2 (TXA2) and biological significance prostanoid (TP) receptor signaling in TNF-α-mediated male C57Bl/6 mice. number leukocytes adhering endothelial cells microvessels (the portal venules, sinusoids, central venules) percentage nonperfused sinusoids were determined using vivo fluorescence microscopy. FR167653, an inhibitor TNF-α, administered 0 2...
Aortic dissection (AD) is the acute destruction of aortic wall and reportedly induced by inflammatory response. Here we investigated role smooth muscle Socs3 (a negative regulator Janus kinases/signal transducer activator transcription signaling) in AD pathogenesis using a mouse model generated via β-aminopropionitrile angiotensin II infusion. deletion specifically cells yielded chronic response wall, which was associated with increased fibroblasts, reinforced tensile strength, less-severe...
Aortic dissection (AD) is a major cause of acute aortic syndrome with high mortality due to the destruction walls. Although recent studies indicate critical role inflammation in disease mechanism AD, it unclear how inflammatory response initiated. Here, we demonstrate that myocardin-related transcription factor A (MRTF-A), signal transducer humoral and mechanical stress, plays an important pathogenesis AD mouse model. model was created by continuous infusion angiotensin II (AngII) induced...
Aortic dissection (AD) is a serious clinical condition that unpredictable and frequently results in fatal outcome. Although rapamycin, an inhibitor of mechanistic target rapamycin (mTOR), has been reported to be effective preventing aortopathies mouse models, its mode action yet clarified. A AD model was created by the simultaneous administration β-aminopropionitrile (BAPN) angiotensin II (AngII) for 14 days. Rapamycin treatment started either at day 1 or 7 BAPN+AngII challenge, continued...
Leukotrienes (LTs), metabolites of arachidonic acid through 5-lipoxygenase (5-LOX), have been known to play a role in leukocyte recruitment. However, the contribution LTB4 liver microcirculatory dysfunction during endotoxemia remains unknown. receptor (BLT1) has identified as high-affinity specific for LTB4. The present study was conducted examine roles and BLT1 hepatic elicited by LPS mice. number leukocytes adhering endothelial cells microvessels perfused sinusoids determined 4 h after...
Angiotensinogen (AGT) is an essential component in the renin-angiotensin system. AGT has highly conserved sequences loop and β-sheet regions among species; however, their functions have not been studied.
Background: Plasminogen activator inhibitor-1 (PAI-1) is highly abundant in human thoracic aortic aneurysms (TAA). This high abundance mimicked TAAs created by chronic infusion of angiotensin II (AngII) into mice. The purpose this study was to determine whether deletion PAI-1 influenced the development during AngII infusion. Methods and Results: To role acute phases AngII-induced pathology, whole-body deficient mice (PAI-1 -/-) wild type littermates +/+) were infused with (1,000 ng/kg/min)...
Background and Aim: Fibrillin-1 has a complex biology that can influence elastic fiber formation stability. Smooth muscle cells (SMCs), the most abundant cell type of aortic wall, synthesize secrete fibrillin-1. The purpose this study was to determine effects fibrillin-1 (FBN1) synthesis in SMCs on pathologies. Methods Results: We generated Fbn1 floxed mice included insertion tdTomato as reporter for gene deletion. Female were bred with male expressing Cre transgene driven by Tagln promotor...
Background: Aortic dissection (AD) confined to the descending thoracic aorta is typically managed conservatively in acute phase. However, these patients often require surgeries due aortic dilatation chronic Although preclinical studies have been conducted investigate mechanism of AD, most focused on phase, especially cause AD. It remains unknown how molecular mechanisms alter following Methods and Results: To determine alteration after bulk RNA sequencing was performed using aortas from mice...
We report three cases of airway management with elective surgical cricothyroidotomy (SCT) for anesthetic during repair maxillofacial injury involving basal skull fracture or nasal-bone fracture. In all patients, general anesthesia was induced, a supraglottic (SGA) device inserted, and SCT performed. Tracheal intubation performed through site, the SGA removed. After surgery fixation, re-inserted tracheal tube No major complications, such as subglottic stenosis voice change, occurred. holds...
The regional heterogeneity of vascular components and transcriptomes is an important determinant aortic biology. This notion has been explored in multiple mouse studies. In the present study, we examined aortas non-human primates.Aortic samples were harvested from ascending, descending, suprarenal, infrarenal regions young control monkeys adult provided with high fructose for 3 years. structure was by histological bulk RNA sequencing analyses.Immunostaining CD31 αSMA revealed that...
The regional heterogeneity of vascular components and transcriptomes is an important determinant aortic biology. This notion has been explored in multiple mouse studies. In the present study, we examined aortas nonhuman primates.