A5037445654- Peptidase Inhibition and Analysis
- Advanced Proteomics Techniques and Applications
- Potato Plant Research
- Sphingolipid Metabolism and Signaling
- Mass Spectrometry Techniques and Applications
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Metabolomics and Mass Spectrometry Studies
- Cancer Cells and Metastasis
- Sulfur Compounds in Biology
- Protein Degradation and Inhibitors
- Plant Surface Properties and Treatments
- Food, Nutrition, and Cultural Practices
- Forensic Fingerprint Detection Methods
- Biochemical and Molecular Research
- Endoplasmic Reticulum Stress and Disease
- Axon Guidance and Neuronal Signaling
- Lipid Membrane Structure and Behavior
- Melanoma and MAPK Pathways
- Wood and Agarwood Research
- Environmental and biological studies
- Redox biology and oxidative stress
- Sesame and Sesamin Research
- Logistics and Infrastructure Analysis
- Histone Deacetylase Inhibitors Research
Universidade Federal do Espírito Santo
2023-2024
University of Lisbon
2016-2024
Hospital da Senhora da Oliveira Guimarães
2023
Institut des Sciences Moléculaires
2022
GlaxoSmithKline (United Kingdom)
2017-2021
Instituto Superior Técnico
2018-2021
Hospital Erasto Gaertner
2021
Instituto Politécnico de Lisboa
2018-2021
Hammersmith Hospital
2015-2016
Imperial College London
2009-2016
Selective covalent inhibition of kinases by targeting poorly conserved cysteines has proven highly fruitful to date in the development chemical probes and approved drugs. However, this approach is limited ∼200 possessing such a cysteine near ATP-binding pocket. Herein, we report novel achieve selective, irreversible kinase inhibition, catalytic lysine residue. We have illustrated our developing PI3Kδ inhibitors that exhibit nanomolar potency cellular assays, duration action >48 h CD4+ T...
Focal adhesion kinase (FAK) is a key mediator of tumour progression and metastasis. To date, clinical trials FAK inhibitors have reported disappointing efficacy for oncology indications. We report the design characterisation GSK215, potent, selective, FAK-degrading Proteolysis Targeting Chimera (PROTAC) based on binder VHL E3 ligase known inhibitor VS-4718. X-ray crystallography revealed molecular basis highly cooperative FAK-GSK215-VHL ternary complex, GSK215 showed differentiated in-vitro...
Sphingosine kinase 1 (SK1) plays an important role in estrogen-dependent breast tumorigenesis, but its regulation is poorly understood. A subset of microRNAs (miRNA, miR) regulated by estrogen and contributes to cellular proliferation cancer progression. Here, we describe that miR-515-5p transcriptionally repressed receptor α (ERα) functions as a tumor suppressor cancer. Its downregulation enhances cell SK1 activity, mediated reduction posttranscriptional repression. Enforced expression...
Abstract It has recently been shown that docetaxel chemotherapy is effective in prolonging life patients with prostate cancer (PCa). We have investigated potential ways of increasing the effectiveness this disease. previously reported sphingosine kinase 1 (SphK1) inhibition a key step docetaxel‐induced apoptosis PC‐3 PCa cell line and pharmacologicalSphK1 chemosensitizing docetaxel‐resistant LNCaP line. In study we addressed mechanism cells identified SphK1‐dependent ‐independent components....
LMTK3 is an oncogenic receptor tyrosine kinase (RTK) implicated in various types of cancer, including breast, lung, gastric, and colorectal cancer. It localized different cellular compartments, but its nuclear function has not been investigated so far. We mapped binding across the genome using ChIP-seq found that events are correlated with repressive chromatin markers. further identified KRAB-associated protein 1 (KAP1) as a partner LMTK3. The LMTK3/KAP1 interaction stabilized by PP1α, which...
// Joao Nunes 1, * , Hua Zhang Nicos Angelopoulos 1 Jyoti Chhetri Clodia Osipo 2 Arnhild Grothey 3 Justin Stebbing Georgios Giamas Department of Surgery and Cancer, Division Imperial College London, Hammersmith Hospital Campus, UK Microbiology Immunology, Cardinal Bernardin Cancer Center Loyola University Chicago, Health Sciences Division, Maywood, Illinois, USA School Life Sciences, Sussex, Brighton, These authors have contributed equally to this work Correspondence to: Giamas, e-mail:...
The gene profile of primary tumors, as well the identification circulating tumor cells (CTCs), can provide important prognostic and predictive information. In this study, our objective was to perform profiling (TGP) in combination with CTC characterization women nonmetastatic breast cancer. Biological samples (from peripheral blood tumors) from 167 patients diagnosed stage I, II, III mammary carcinoma, who were also referred for adjuvant/neoadjuvant chemotherapy, assessed following...
The ‘gasotransmitters’ hydrogen sulfide (H2S), nitric oxide (NO), and carbon monoxide (CO) act as second messengers in human physiology, mediating signal transduction via interaction with or chemical modification of protein targets, thereby regulating processes such neurotransmission, blood flow, immunomodulation, energy metabolism. Due to their broad reactivity potential toxicity, the biosynthesis breakdown H2S, NO, CO are tightly regulated. Growing evidence highlights active role...
Goldgerg de que existe uma linguagem comum para o distresse psicológico entre culturas.
Kinase suppressor of Ras 1 (KSR1) has been implicated in tumorigenesis multiple cancers, including skin, pancreatic and lung carcinomas. However, our recent study revealed a role KSR1 as tumour breast cancer, the expression which is potentially correlated with chemotherapy response. Here, we aimed to further elucidate KSR1-regulated signalling response genotoxic agents cancer. Stable isotope labelling by amino acids cell culture (SILAC) coupled high-resolution mass spectrometry (MS) was...
Acrylamide and furan are environmental food contaminants that metabolized by cytochrome P450 2E1 (CYP2E1), giving rise to glycidamide cis-2-butene-1,4-dial (BDA) metabolites, respectively. Both BDA electrophilic species react with nucleophilic groups, being able introduce mutations in DNA perform epigenetic remodeling. However, whereas these carcinogens primarily the liver, carcinogenic potential of acrylamide this organ is still controversial, based on findings from experimental animal...
Cervical cancer is the fourth most common in women worldwide. Epidemiological and quality of life (QoL) data patients with cervical from low- middle-income countries are scarce. We aimed to describe sociodemographic clinicopathological characteristics at diagnosis Brazil.EVITA a prospective cohort study newly diagnosed May 2016 December 2017, stages I-IVB, 16 Brazilian sites representing five regions. At baseline, medical evaluation was performed European Organization for Research Treatment...
Identification of protein covalent modifications (adducts) is a challenging task mainly due to the lack data processing approaches for adductomics studies. Despite huge technological advances in mass spectrometry (MS) instrumentation and bioinformatics tools proteomics studies, these methodologies have very limited success on identification low abundant adducts. Herein we report novel strategy inspired metabolomics workflows covalently-modified peptides that consists LC-MS preprocessing...
Abstract Focal adhesion kinase (FAK) is a key mediator of tumour progression and metastasis. To date, clinical trials FAK inhibitors have reported disappointing efficacy for oncology indications. We report the design characterisation GSK215, potent, selective, FAK‐degrading Proteolysis Targeting Chimera (PROTAC) based on binder VHL E3 ligase known inhibitor VS‐4718. X‐ray crystallography revealed molecular basis highly cooperative FAK‐GSK215‐VHL ternary complex, GSK215 showed differentiated...
Elevated levels of the estrone metabolite, 16α-hydroxyestrone (16αOHE1), have been linked with multiple diseases. As an electrophilic reactive covalent binding to proteins is thought constitute one possible mechanisms in onset deleterious health outcomes associated 16αOHE1. Whereas mass spectrometry (MS)-based methodologies are currently considered best suited monitor formation protein adducts, application these approaches for identification adducts 16αOHE1 yet be provided. In present study,...
A utilização de aeronaves remotamente pilotadas (ARPs) no setor agrícola, em operações pulverização e aplicação defensivos agrícolas fertilizantes, está constante expansão. Com isso, a necessidade pesquisas técnicos-científicas se faz cada vez mais evidente. O objetivo foi realizar um levantamento informações referentes à temática, utilizando análise bibliométrica. Essa conduzida partir das publicações científicas obtidas pela base dados Scopus, termos como UAV, UAS, drone, depósito, RPA,...
Análise bibliométrica é um importante método de pesquisa que visa compilar produções científicas quantitativamente uma determinada área conhecimento, em período tempo. Dentre as características relacionadas ao tema destacam-se número artigos, instituições, autores, citações, palavras-chave, entre outros indicadores. Objetivou-se neste trabalho reunir informações os anos 2013 a 2023 sobre integração drones, inteligência artificial e internet das coisas no controle sustentável doenças nas...
Pinus pinaster forestry occupies >20% of the forest ecosystem area in continental territory Portugal with a high impact on national economy. This species’ major derived non-wood product is oleoresin, raw material for rosin production. Rosin comprises mainly blend resin acids and has broad industrial pharmaceutical applications. Oleoresin production been progressively reduced due to low-cost producers other countries; currently, it reaches only 2% existing P. trees. To support this...
Abstract LMTK3 is an oncogenic receptor tyrosine kinase implicated in various types of cancer including breast, lung, gastric and colorectal. We have already demonstrated the contribution invasion, migration transcriptional regulation as well its involvement endocrine resistance breast cancer. Despite significant progress understanding role human tumourogenesis, signalling pathways still remain to be elucidated. Protein phosphorylation play essential regulating intracellular signal...