A5010246246- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Electrospun Nanofibers in Biomedical Applications
- Telomeres, Telomerase, and Senescence
- Glaucoma and retinal disorders
- RNA Interference and Gene Delivery
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Ocular Surface and Contact Lens
- Molecular Biology Techniques and Applications
Biologie, ingénierie et imagerie pour l'Ophtalmologie
2025
Université Jean Monnet
2022-2025
Laboratoire de Chimie
2022
Aims/Purpose: Corneal storage in organ culture (OC) at 31‐34°C is the reference method Europe. In France, eye banks have traditionally used CorneaMax/CorneaJet media (Eurobio, Les Ulis, France) for several decades. Recently, Tissue‐C/Carry‐C (Alchimia‐Moria, Antony, became available. This study aims comparing quality of corneas stored these two media. Methods: Eye Saint Etienne and Rouen participated study. Rouen, experiments were conducted. The first included 18 pairs corneas, with...
Aims/Purpose: In response to the global shortage of corneas, alternatives such as cell injection therapy and tissue‐engineered endothelial keratoplasty (TEEK) have emerged, both relying on mass production primary cultured corneal cells (CECs). Cryopreservation corneas (the culture source), CECs (in vitro), TEEK grafts (final products) is crucial facilitate their industrialization clinical application. While cryopreservation has long been a challenge, advancements in cryoprotectants warrant...
Aims/Purpose: Due to the global shortage of corneal donors, alternatives such as cell injection therapy and tissue‐engineered endothelial keratoplasty are being developed. These methods require mass production cells (CECs), which is challenging, especially with corneas from "regular" donors (aged around 70 on average in Europe). Notably, first clinical successes reported by Kinoshita et al. used under 29, rare. This study aims at investigating success rate specific challenges primary...
Aims/Purpose: Corneal transplantation is currently the most frequent type of globally. However, a chronic shortage donor corneas makes traditional methods unsustainable. Alternatives such as bio‐engineered grafts or direct injection corneal endothelial cells (CECs) into patient's eye require primary culture CECs. Current CEC techniques face significant challenge: cell senescence. Cells often become senescent during in vitro culture, rendering them unsuitable for clinical use and limiting...
Aims/Purpose: In response to the global shortage of corneas, alternatives such as cell injection therapy and tissue‐engineered endothelial keratoplasty (TEEK) have emerged, both relying on mass production primary cultured corneal cells (CECs). Cryopreservation corneas (the culture source), CECs (in vitro), TEEK grafts (final products) is crucial facilitate their industrialization clinical application. While cryopreservation has long been a challenge, advancements in cryoprotectants warrant...
Aims/Purpose: Corneal transplantation is currently the most frequent type of globally. However, a chronic shortage donor corneas makes traditional methods unsustainable. Alternatives such as bio‐engineered grafts or direct injection corneal endothelial cells (CECs) into patient's eye require primary culture CECs. Current CEC techniques face significant challenge: cell senescence. Cells often become senescent during in vitro culture, rendering them unsuitable for clinical use and limiting...
This study addressed limitations in calcein-AM-based endothelial viability assays, specifically focusing on pre-stripped DMEK grafts. Key challenges included the suboptimal calcein staining and incompatibility of assay with subsequent immunofluorescence (IF). Using human corneal grafts, we employed two strategies to optimize staining. Firstly, improved endothelium by adjusting calcein-AM concentration diluent, resulting a threefold increase fluorescence intensity 4 µM Opti-MEM compared...
Corneal endothelial diseases are the leading cause of corneal transplantation. The global shortage donor corneas has resulted in investigation alternative methods, such as cell therapy and tissue-engineered keratoplasty (TEEK), using primary cultures human cells (hCECs). main challenge is optimizing hCEC culture process to increase density (ECD) overall yield while preventing endothelial-mesenchymal transition (EndMT). Fetal bovine serum (FBS) necessary for expansion but contains TGF-βs,...
Keratoconus (KC) is a multifactorial progressive ectatic disorder characterized by local thinning of the cornea, leading to decreased visual acuity due irregular astigmatism and opacities. Despite evolution advanced imaging methods, exact etiology KC remains unknown. Our aim was investigate involvement corneal epithelium in pathophysiology disease. Corneal epithelial samples were collected from 23 controls 2 cohorts patients with KC: 22 undergoing crosslinking (early KC) 6 before penetrating...
The quality of the endothelial graft is critical to success DMEK and survival time graft. peeling technique, preservation method, skill level preparers need be evaluated validated. most reliable method evaluation viability test based on a triple staining Hoechst- Ethidium-Calcein AM (H-E-C) which allows determination total number viable cells However, this has some shortcomings for grafts: 1) undesirable fluorescence Calcein stain prevents accurate analysis, especially in cases where...
Abstract Purpose Quality of the endothelial transplant is a critical parameter in success Descemet Membrane Endothelial Keratoplasty (DMEK) and graft survival. The peeling techniques, preservation methods operator’s skill level need to be experimentally assessed validated, as they are key elements influencing quality. most reliable method quality evaluation triple staining with Hoechst‐Calcein AM‐Ethidium (HEC) allowing determine total number viable cells. However, test HEC has defects: (1)...
Abstract Purpose: The corneas preserved in bioreactor (BR) had been shown to have not only a better endothelial viability, but also more differentiated and stratified epithelium than organoculture. By using BR, we would analyse the respective contribution of corneal (C), limbal (L), conjunctival (Conj) epithelia epithelial regeneration. Methods: Five pairs from body donation Science were used with death‐to‐collection time < 20 h. A 3‐ 5‐mm‐wide flange was kept intact. patterns set up by...