A5050966284- Corneal Surgery and Treatments
- Spectroscopy Techniques in Biomedical and Chemical Research
- Electrospun Nanofibers in Biomedical Applications
- Connexins and lens biology
- Olfactory and Sensory Function Studies
- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Intraocular Surgery and Lenses
- Biochemical Analysis and Sensing Techniques
- Periodontal Regeneration and Treatments
- Biological Stains and Phytochemicals
- Neurobiology and Insect Physiology Research
- Kidney Stones and Urolithiasis Treatments
Biologie, ingénierie et imagerie pour l'Ophtalmologie
2021-2025
Laboratoire d’Imagerie Biomédicale
2025
Université Jean Monnet
2021-2023
Aims/Purpose: In response to the global shortage of corneas, alternatives such as cell injection therapy and tissue‐engineered endothelial keratoplasty (TEEK) have emerged, both relying on mass production primary cultured corneal cells (CECs). Cryopreservation corneas (the culture source), CECs (in vitro), TEEK grafts (final products) is crucial facilitate their industrialization clinical application. While cryopreservation has long been a challenge, advancements in cryoprotectants warrant...
Aim: Establishing an ex vivo model of the crystalline lens will be a driving force for further research on this tissue. The epithelium plays essential role in maintaining osmolarity, volume, and transparency. Therefore, it is crucial to have tool control vivo. We previously validated method quantifying epithelial cell viability whole lenses by triple labeling with Hoechst 33342, ethidium homodimer calcein‐AM (HEC) using acute frostbite necrosis. now demonstrate application chemical toxicity...
Aims/Purpose: In response to the global shortage of corneas, alternatives such as cell injection therapy and tissue‐engineered endothelial keratoplasty (TEEK) have emerged, both relying on mass production primary cultured corneal cells (CECs). Cryopreservation corneas (the culture source), CECs (in vitro), TEEK grafts (final products) is crucial facilitate their industrialization clinical application. While cryopreservation has long been a challenge, advancements in cryoprotectants warrant...
Aim: Establishing an ex vivo model of the crystalline lens will be a driving force for further research on this tissue. The epithelium plays essential role in maintaining osmolarity, volume, and transparency. Therefore, it is crucial to have tool control vivo. We previously validated method quantifying epithelial cell viability whole lenses by triple labeling with Hoechst 33342, ethidium homodimer calcein‐AM (HEC) using acute frostbite necrosis. now demonstrate application chemical toxicity...
Abstract The monolayer of approximately 300,000 human corneal endothelial cells (hCECs) on the posterior surface cornea is essential to maintain transparency but non-self-regenerative. Corneal blindness can currently only be treated by transplantation, hindered a global donor shortage, highlighting need for developing tissue and/or cell therapy. mass production these advanced therapy medicinal products requires obtaining high-yield, high-quality culture characterized hexagonal shape, low...
Corneal endothelial diseases are the leading cause of corneal transplantation. The global shortage donor corneas has resulted in investigation alternative methods, such as cell therapy and tissue-engineered keratoplasty (TEEK), using primary cultures human cells (hCECs). main challenge is optimizing hCEC culture process to increase density (ECD) overall yield while preventing endothelial-mesenchymal transition (EndMT). Fetal bovine serum (FBS) necessary for expansion but contains TGF-βs,...
The pathophysiology underlying olfactory dysfunction is still poorly understood, and more efficient biomolecular tools are necessary to explore this aspect. Immunohistochemistry (IHC) on cross sections one of the major study epithelium (OE), but does not allow reliable counting sensory neurons (OSNs) or cartography OE. In study, we want present an easy immunostaining technique compensate for these defects IHC. Using rat model, first validated pre-screened key OSN markers by IHC Tuj-1, OMP,...
Purpose: The lens has a monolayer of epithelial cells on its anterior surface that contributes to homeostasis. A quantification the viability these is essential evaluate safety new therapies targeting (cataract, presbyopia). observation this whole tissue made difficult by optical properties (transparency and refraction) only simplified models have been used until now measure (primary cell cultures or partial surgical specimens). Aims: To develop method quantify ex vivo lenses. Methods: Ten...
Human corneas preserved in bioreactor (BR) are characterized by not only a better endothelial viability, but also more differentiated and stratified epithelium compared to organoculture. By using corneal preservation BR, we aimed analyze the respective contribution of (C), limbal (L), conjunctival (Conj) epithelia epithelial regeneration.Five pairs from body donation Science were used with death-to-collection time <20 hours. A 3- 5-mm-wide flange was kept intact. Five patterns set up...
Abstract Purpose: The corneas preserved in bioreactor (BR) had been shown to have not only a better endothelial viability, but also more differentiated and stratified epithelium than organoculture. By using BR, we would analyse the respective contribution of corneal (C), limbal (L), conjunctival (Conj) epithelia epithelial regeneration. Methods: Five pairs from body donation Science were used with death‐to‐collection time < 20 h. A 3‐ 5‐mm‐wide flange was kept intact. patterns set up by...
Abstract A classification of olfactory sensory neuron (OSN) markers, a simple and robust technique immunostaining on flat-mounted epithelium (OE) reliable quantification the density mature immature OSNs are three crucial tools to study pathophysiology dysfunction. Using rat model, we first categorized main OSN markers by immunohistochemistry (IHC) cross sections OE. The were divided into 3 groups: (OMP), (Tuj-1, DCX, OLIG2) both (N-cadherin, LHX2, PGP9.5). subcellular localization each...