A5058947319- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Intraocular Surgery and Lenses
- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Ocular Diseases and Behçet’s Syndrome
- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Nonmelanoma Skin Cancer Studies
- Retinal Imaging and Analysis
- Cutaneous Melanoma Detection and Management
- Optical Coherence Tomography Applications
- Ocular Oncology and Treatments
- Retinal and Macular Surgery
- Ophthalmology and Visual Impairment Studies
- Connexins and lens biology
- Herpesvirus Infections and Treatments
- Cancer and Skin Lesions
- Traumatic Ocular and Foreign Body Injuries
- Angiogenesis and VEGF in Cancer
- Bacterial Identification and Susceptibility Testing
- Spectroscopy Techniques in Biomedical and Chemical Research
- Optical Imaging and Spectroscopy Techniques
- Ocular and Laser Science Research
Centre Hospitalier Universitaire de Saint-Étienne
2016-2025
Université Jean Monnet
2016-2025
Biologie, ingénierie et imagerie pour l'Ophtalmologie
2016-2025
Institute Cancer De La Loire Lucien Neuwirth
2021-2025
Laboratoire d’Imagerie Biomédicale
2025
Institut de France
2015-2024
Institut Universitaire de France
2013-2022
Laboratoire de Chimie
2022
Hôpital Nord
2012-2020
Mike O'Callaghan Federal Medical Center
2020
Corneal transplantation restores visual function when impairment caused by a corneal disease becomes too severe. It is considered the world's most frequent type of transplantation, but, to our knowledge, there are no exhaustive data allowing measurement supply and demand, although such essential in defining local, national, global strategies fight blindness.To describe worldwide situation demand.Data were collected between August 2012 2013 from systematic review published literature parallel...
The control of corneal transparency depends on the integrity its endothelial monolayer, which is considered nonregenerative in adult humans. In pathological situations, cell (EC) loss, not offset by mitosis, can lead to irreversible edema and blindness. However, hypothesis a slow, clinically insufficient regeneration starting from periphery remains debatable. authors have re-evaluated microanatomy endothelium order identify structures likely support this homeostasis model. Whole endothelia...
Maintenance of corneal transparency is crucial for vision and depends mainly on the endothelium, a non-proliferative monolayer cells covering inner part cornea. When endothelial cell density falls below critical threshold, barrier “pump” functions endothelium are compromised which results in oedema loss visual acuity. The conventional treatment such severe disorder graft. Unfortunately, there worldwide shortage donor corneas, necessitating amelioration tissue survival storage after...
Background The assessment of blood lipids is very frequent in clinical research as it assumed to reflect the lipid composition peripheral tissues. Even well accepted such relationships have never been clearly established. This particularly true ophthalmology where use has become common following recent data linking intake ocular health and disease. In present study, we wanted determine humans whether a lipidomic approach based on red cells could reveal associations between circulating tissue...
Abstract Corneal endothelial cells (CECs) are terminally differentiated cells, specialized in regulating corneal hydration and transparency. They highly polarized flat that separate the cornea from aqueous humor. Their apical surface, contact with humor is hexagonal, whereas their basal surface irregular. We characterized structure of human CECs 3D using confocal microscopy immunostained whole corneas which interrelationships remain intact. Hexagonality was maintained by interaction between...
Abstract Retinal melanosome/melanolipofuscin-containing cells (MCCs), clinically visible as hyperreflective foci (HRF) and a highly predictive imaging biomarker for the progression of age-related macular degeneration (AMD), are widely believed to be migrating retinal pigment epithelial (RPE) cells. Using human donor tissue, we identify vast majority MCCs melanophages, melanosome/melanolipofuscin-laden mononuclear phagocytes (MPs). serial block-face scanning electron microscopy, RPE...
To evaluate eubacterial PCR compared with conventional cultures for detection and identification of bacterial agents in ocular samples from patients acute postcataract endophthalmitis.Broad-range amplification was used, followed by direct DNA sequencing (aqueous humor, vitreous tap or vitrectomy) 100 consecutive presenting endophthalmitis. Bacterial were performed on the same using traditional methods (brain-heart infusion broth).At time admission, rate not significantly different between...
To assess to which extent the COVID-19 pandemic affected corneal transplantation by virtue of donor selection algorithms in different European countries.Survey.110 eye banks 26 countries.64 covering 95% activity.A questionnaire listing number corneas procured and distributed from February May 2018-2020 was circulated banks.The primary outcome procurements. Additional outcomes were national for selection, classified according their stringency (donors with history, suspected COVID-19,...
Aims/Purpose: Corneal storage in organ culture (OC) at 31‐34°C is the reference method Europe. In France, eye banks have traditionally used CorneaMax/CorneaJet media (Eurobio, Les Ulis, France) for several decades. Recently, Tissue‐C/Carry‐C (Alchimia‐Moria, Antony, became available. This study aims comparing quality of corneas stored these two media. Methods: Eye Saint Etienne and Rouen participated study. Rouen, experiments were conducted. The first included 18 pairs corneas, with...
Aims/Purpose: Due to the global shortage of corneal donors, alternatives such as cell injection therapy and tissue‐engineered endothelial keratoplasty are being developed. These methods require mass production cells (CECs), which is challenging, especially with corneas from "regular" donors (aged around 70 on average in Europe). Notably, first clinical successes reported by Kinoshita et al. used under 29, rare. This study aims at investigating success rate specific challenges primary...
Aim: Establishing an ex vivo model of the crystalline lens will be a driving force for further research on this tissue. The epithelium plays essential role in maintaining osmolarity, volume, and transparency. Therefore, it is crucial to have tool control vivo. We previously validated method quantifying epithelial cell viability whole lenses by triple labeling with Hoechst 33342, ethidium homodimer calcein‐AM (HEC) using acute frostbite necrosis. now demonstrate application chemical toxicity...
Aims/Purpose: Corneal transplantation is currently the most frequent type of globally. However, a chronic shortage donor corneas makes traditional methods unsustainable. Alternatives such as bio‐engineered grafts or direct injection corneal endothelial cells (CECs) into patient's eye require primary culture CECs. Current CEC techniques face significant challenge: cell senescence. Cells often become senescent during in vitro culture, rendering them unsuitable for clinical use and limiting...
Purpose: Fuchs endothelial corneal dystrophy (FEDC) is by far the most common diseases in Western countries, and 50% of keratoplasties are carried out to treat diseases. New treatments also emerging (Descemetorhexis only, rock‐inhibitors, mTor‐inhibitors, FGF‐1). It will therefore soon be vital able determine stage each patient a simple reliable way, order personalize treatment. Visual acuity, retroillumination, specular microscopy thickness mapping 4 pillars examination. However, current...
Aims/Purpose: The development of cell therapies to treat corneal endothelial pathologies requires characterizing the cells during culture process. density (ECD) and morphometry are essential parameters for identifying cultures with sufficient phenotype yield. However, they difficult measure on in using conventional image analysis tools. Aims: (1) To develop an AI‐based automatic segmentation method reliable ECD morphology measurement. (2) select new parameters. (3) classify by quality....
Aims/Purpose: The risk of transmission systemic diseases from donor to recipient is particularly low, since in 50 years and 2.5 million transplants, only 8 cases rabies, 2 hepatitis B Creuzfeldt‐Jakob disease (CJD) have been well documented. Conversely, other HIV, hep C, CJD not transmitted. Nevertheless, the list contraindications (CI) donation also includes for which no has identified: neurodegenerative diseases, haematological malignancies, melanomas, tumours central nervous system...
Aims/Purpose: Corneal transplantation is currently the most frequent type of globally. However, a chronic shortage donor corneas makes traditional methods unsustainable. Alternatives such as bio‐engineered grafts or direct injection corneal endothelial cells (CECs) into patient's eye require primary culture CECs. Current CEC techniques face significant challenge: cell senescence. Cells often become senescent during in vitro culture, rendering them unsuitable for clinical use and limiting...
Aim: Establishing an ex vivo model of the crystalline lens will be a driving force for further research on this tissue. The epithelium plays essential role in maintaining osmolarity, volume, and transparency. Therefore, it is crucial to have tool control vivo. We previously validated method quantifying epithelial cell viability whole lenses by triple labeling with Hoechst 33342, ethidium homodimer calcein‐AM (HEC) using acute frostbite necrosis. now demonstrate application chemical toxicity...
Aims/Purpose: Fuchs endothelial corneal dystrophy is currently the main indication for keratoplasty. Alternatively, a localized 4mm Descemet Stripping Only (DSO) has been proposed to treat selected patients with sufficient peripheral reserve (1,2). Visual recovery generally longer than after keratoplasty but no immunosuppressive treatment needed (3). The anterior lens capsule (ALC) can be used as support culture of cells (CECs)(4). Their migration facilitated by presence basal membrane. Aim:...
This study addressed limitations in calcein-AM-based endothelial viability assays, specifically focusing on pre-stripped DMEK grafts. Key challenges included the suboptimal calcein staining and incompatibility of assay with subsequent immunofluorescence (IF). Using human corneal grafts, we employed two strategies to optimize staining. Firstly, improved endothelium by adjusting calcein-AM concentration diluent, resulting a threefold increase fluorescence intensity 4 µM Opti-MEM compared...
Dorado Cortez, Oliver MD; Gain, Philippe MD, PhD; Thuret, Gilles PhD Author Information
<b>Background:</b> Endothelial examination of organ culture stored corneas is usually done manually and on several mosaic zones. Some banks use an image analyser that takes account only one zone. This method restricted by quality, may be inaccurate if endothelial cell density (ECD) within the not homogeneous. The authors have developed has tools for automatic error detection correction, can measure ECD perform morphometry multiple zones three images mosaic. <b>Methods:</b> 60 human were...