A5012411270- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiomyopathy and Myosin Studies
- Cardiovascular Effects of Exercise
- Receptor Mechanisms and Signaling
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Neuroscience and Neuropharmacology Research
- Cardiac pacing and defibrillation studies
- Cardiovascular Function and Risk Factors
- Pharmacological Receptor Mechanisms and Effects
- Nitric Oxide and Endothelin Effects
- Photosynthetic Processes and Mechanisms
- Neuropeptides and Animal Physiology
- RNA Research and Splicing
- Blood Coagulation and Thrombosis Mechanisms
- Plant Reproductive Biology
- ATP Synthase and ATPases Research
- Veterinary Pharmacology and Anesthesia
- Cardiac Arrhythmias and Treatments
- Alzheimer's disease research and treatments
- Plant Molecular Biology Research
- Birth, Development, and Health
- Neuroscience and Neural Engineering
- Pregnancy and preeclampsia studies
Wageningen University & Research
2024
Max Delbrück Center
1994-2016
Jefferson College
1997
Most of the patients with congenital heart diseases express atrial myosin light chain 1 (ALC-1) in right ventricle. We investigated functional consequences ALC-1 expression on cycling kinetics intact sarcomeric structure using multicellular demembranated fibers ("skinned fibers") from ventricular infundibulum Tetralogy Fallot (TOF), double outlet ventricle (DORV), and infundibular pulmonary stenosis (IPS), Force-velocity relation was analyzed by constant-load technique at maximal Ca2+...
Mechanical properties and isoform composition of myosin heavy light chains were studied in hypertrophying rat urinary bladders. Growth the bladder was induced by partial ligation urethra. Preparations obtained after 10 days. In maximally activated skinned preparations from tissue, maximal shortening velocity rate force development following photolytic release ATP reduced about 20 25%, respectively. Stiffness unchanged. The relative content basic essential 17 kDa chain doubled hypertrophied...
The N terminus of myosin light chain 1 (MLC‐1) skeletal muscle bind to the C actin. We investigated whether termini human cardiac MLC‐1 isoforms likewise Furthermore, we N‐terminal sequence 5–15 (P5–14) atrium (ALC‐1) and ventricle (VLC‐1) with different affinities Affinity beads were produced by covalently coupling a synthetic peptide corresponding 4–14 VLC‐1 aminohexylagarose in order G‐actin. found, that G‐actin specifically binds affinity beads. preincubation P5‐14 both ALC‐1 decreased...
AimsWe tested the hypothesis that mutations in human ventricular essential myosin light chain (hVLC-1) are associated with hypertrophic cardiomyopathy (HCM) affect protein structure, binding to IQ1 motif of cardiac heavy (MYH) and sarcomeric sorting neonatal cardiomyocytes.
Smooth muscle in megacolon was studied the lethal spotted mouse and its normal sibling. In megacolon, structural remodeling a very large increase total protein content are associated with some changes contractile isoform composition. 1) There is higher actin concentration primarily caused by larger proportion of gamma-isoforms. 2) no difference myosin or SM1/SM2 heavy chains proteins. 3) Only LC17a essential light chain present both megacolon. 4) The 25- to 50-kDa 5'-insert occurs 15-20%...
Embryo development in Arabidopsis (Arabidopsis thaliana) starts off with an asymmetric division of the zygote to generate precursors embryo proper and supporting extraembryonic suspensor. The suspensor degenerates as proceeds beyond heart stage. Until globular stage, maintains embryonic potential can form embryos absence developing proper. We report a mutant called meerling-1 (mrl-1), which shows high penetrance suspensor-derived polyembryony due delayed Eventually, from both apical lineages...
Plakoglobin (γ-catenin), a member of the armadillo family proteins, is constituent cytoplasmic plaque cardiac junctions and involved in anchorage cytoskeletal filaments to specific cadherins. Its genetic inactivation leads an embryonic lethal phenotype due heart dysfunction related impairment architecture intercalated discs stability tissue. To elucidate functional consequences loss plakoglobin for myofibrillar function, we monitored passive stress–strain relationship contractility...