A5061745188- Receptor Mechanisms and Signaling
- Lipid Membrane Structure and Behavior
- Extracellular vesicles in disease
- Analytical Chemistry and Sensors
- Blood properties and coagulation
- Water Treatment and Disinfection
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Gene expression and cancer classification
- Clinical Laboratory Practices and Quality Control
- Advanced Fluorescence Microscopy Techniques
- Wastewater Treatment and Nitrogen Removal
- Neurological disorders and treatments
- bioluminescence and chemiluminescence research
- MicroRNA in disease regulation
- Meta-analysis and systematic reviews
- Olfactory and Sensory Function Studies
- Advanced biosensing and bioanalysis techniques
- Nanopore and Nanochannel Transport Studies
- Microbial Community Ecology and Physiology
- Neurobiology and Insect Physiology Research
- Genomics and Chromatin Dynamics
- Molecular Junctions and Nanostructures
- Nanoplatforms for cancer theranostics
- Genomic variations and chromosomal abnormalities
AC Immune (Switzerland)
2018
École Polytechnique Fédérale de Lausanne
2010-2017
We report on a generic method to detect and identify the molecular profile of exosomes either derived from cultured cell lines or isolated biofluids. Exosomes are nanovesicles shed by cells into their microenvironment carry identity mother cells. These vesicles actively involved in intercellular communication under physiological conditions ultimately spread various diseases such as cancer. As they accessible most biofluids (e.g., blood, urine, saliva), these biological entities promising...
Cells secrete extracellular vesicles (EVs) into their microenvironment that act as mediators of intercellular communication under physiological conditions and in this context also actively participate spreading various diseases. Large efforts are currently made to produce reliable EV samples develop, improve, standardize techniques allowing biophysical characterization. Here, we used ultrafiltration size-exclusion chromatography for the isolation a model-free fluorescence fluctuation...
Running cold and hot water in buildings is a widely established commodity. However, interests regarding hygiene microbiological aspects had so far been focussed on water. Little attention has given to the microbiology of domestic hot-water installations (DHWIs), except for pathogenic Legionella. World-wide, regulations consider (or warm) as 'heated drinking water' that must comply (cold) (DW) standards. few reports exist indicate presence growth microbial flora DHWIs, even when supplied with...
Olfactory receptors (ORs) constitute the largest family of sensory membrane proteins in mammals. They play a key role within olfactory system recognizing and discriminating nearly unlimited number structurally diverse odorous molecules. The molecular basis OR-mediated signal detection transduction is poorly understood. This due to difficulties functional expression ORs high yields, preventing structural biophysical studies at level receptor protein. Here we report on recombinant mouse...
Cellular signaling is classically investigated by measuring optical or electrical properties of single populations living cells. Here we show that ligand binding to cell surface receptors and subsequent activation cascades can be monitored in single, (sub-)micrometer sized native vesicles with single-molecule sensitivity. The are derived from live mammalian cells using chemicals tweezers. They comprise parts a cell's plasma membrane cytosol represent the smallest autonomous containers...
Abstract Tissue transglutaminase (tTGase) catalyzes both deamidation and transamidation of peptides proteins by using a peptidyl glutamine as primary substrate. A precise consensus sequence for the enzyme is unknown ratio between deamidated transamidated (or cross‐linked) reaction products highly substrate‐dependent. Due to its overlapping body distribution with tTGase ease manipulation tandem mass spectrometry, we used neuropeptide substance P model investigate associated enzymatic kinetics...
Automated flow cytometry (FCM) adapted to real-time quality surveillance provides high4 temporal-resolution data about the microbial communities in a water system. The cell concentration calculated from FCM measurements indicates sudden increases number of bacteria, but can fluctuate significantly due man-made and natural dynamics; it thus obscure presence anomalies. Cytometric fingerprinting tools enable detailed analysis aquatic communities, could distinguish between normal abnormal...
Cheap and massively parallel methods to assess the DNA-binding specificity of transcription factors are actively sought, given their prominent regulatory role in cellular processes diseases. Here we evaluated use protein-binding microarrays (PBM) probe association tumor suppressor AP2α with 6000 human genomic DNA sequences. We show that PBM provides accurate relative binding affinities when compared quantitative surface plasmon resonance assays. A PBM-based study healthy breast tissue...
Keywords: arrestin ; cell-derived plasma membrane vesicle detergent drug delivery FRET fluorescence correlation spectroscopy microscopy G-protein-coupled receptor G-protein activation giant unilamellar ligand binding protein micelle model system optical tweezer surface plasmon resonance transmembrane signaling These Ecole polytechnique federale de Lausanne EPFL, n° 5394 (2012)Programme doctoral Chimie et Genie chimiqueFaculte des sciences baseInstitut ingenierie chimiquesLaboratoire chimie...
The accumulation of aggregated alpha-synuclein (aSyn) in form Lewy bodies and neurites is the pathognomonic signature patients with Parkinson's disease (PD) other synucleinopathies. Currently, diagnosis PD synucleinopathies largely based on symptomatic evaluation patients. direct association between progressive formation aSyn inclusions severity clinical symptoms supports development PET tracers for non-invasive examination aggregates human brain as an objective diagnostic tool applications....