A5016337580- Biosensors and Analytical Detection
- Fecal contamination and water quality
- Advanced biosensing and bioanalysis techniques
- Water Treatment and Disinfection
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Aquaculture disease management and microbiota
- Pharmaceutical and Antibiotic Environmental Impacts
- Vibrio bacteria research studies
- Animal Genetics and Reproduction
- Genetically Modified Organisms Research
- Ionic liquids properties and applications
- Spectroscopy and Chemometric Analyses
- Immune Response and Inflammation
- Identification and Quantification in Food
- Coastal wetland ecosystem dynamics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Maritime Navigation and Safety
- Antibiotic Resistance in Bacteria
- Aquatic Ecosystems and Phytoplankton Dynamics
- Wastewater Treatment and Reuse
- Microfluidic and Bio-sensing Technologies
- SARS-CoV-2 detection and testing
- Antimicrobial Peptides and Activities
- Cassava research and cyanide
Karl Landsteiner University of Health Sciences
2023-2025
TU Wien
2014-2025
Interuniversity Cooperation Centre Water & Health
2017-2024
Universitätsklinikum Tulln
2014-2020
BOKU University
2020
Czech Academy of Sciences, Institute of Analytical Chemistry
2014
The global spread of antimicrobial resistance (AMR) in the environment is a growing health threat. Large rivers are particular concern as they highly impacted by wastewater discharge while being vital lifelines serving various human needs. A comprehensive understanding occurrence, and key drivers AMR along whole river courses largely lacking. We provide holistic approach studying spatiotemporal patterns hotspots antibiotic genes (ARGs) 2311 km navigable Danube River, combining longitudinal...
Faecal pollution of water and the resulting potential presence human enteric pathogens is a predominant threat to public health. Microbiological quality can be assessed by detection standard faecal indicator bacteria (SFIB) such as E. coli or certain Enterococcus species. In recent years, isothermal amplification methods have become useful alternative polymerase chain reaction (PCR), allowing molecular diagnostics with simple no instrumentation. this study, novel screening method for spp....
Molecular diagnostic tools in the field of food and water quality analysis are becoming increasingly widespread. Usually, based on DNA amplification techniques such as polymerase chain reaction (PCR), these methods highly sensitive versatile but require well-equipped laboratories trained personnel. To reduce time avoid expensive equipment, isothermal for detecting various target organisms have been developed. However, to make molecular diagnostics suitable low-resource settings in-field...
Abstract DNA aptamers generated by cell-SELEX against bacterial cells have gained increased interest as novel and cost-effective affinity reagents for cell labelling, imaging biosensing. Here we describe the selection identification of using a combined approach based on cell-SELEX, state-of-the-art applications quantitative real-time PCR (qPCR), next-generation sequencing (NGS) bioinformatic data analysis. This is demonstrated Enterococcus faecalis ( E. ), which served target in eleven...
Human norovirus (HNoV) and human adenovirus (HAdV) are major causes of acute viral gastroenteritis globally environmentally transmitted via the faecal-oral route through contaminated food water. Recent evidence these enteric viruses residing within environmental free-living amoebae (FLA) specifically Vermamoeba vermiformis, Acanthamoeba polyphaga, Willaertia magna has significant implications for virology public health. The incorporation HNoV into cytoplasm vacuoles V. vermiformis A. as well...
The extraction of nucleic acids from microorganisms for subsequent molecular diagnostic applications is still a tedious and time-consuming procedure. We developed method the rapid preparation genomic DNA bacteria based on hydrophilic ionic liquids (ILs). First, we tested eight ILs in different buffer systems their inhibitory effects quantitative PCR. cell lysis potential IL/buffer combinations was assessed by application Enterococcus faecalis as model organism Gram-positive bacteria. two...
Abstract Over the last decades, various PCR-based methods have been proposed that can identify sources of faecal pollution in environmental waters. These microbial source tracking (MST) are powerful tools to manage water quality and support public health risk assessment. However, their application is limited by lack specialized equipment trained personnel laboratories performing microbiological Here, we describe a novel molecular method combines helicase-dependent amplification (HDA) with...
The contribution of ships to the microbial faecal pollution status water bodies is largely unknown but frequently human health concern. No methodology for a comprehensive and target-orientated system analysis was available so far. We developed novel approach integrated multistage impact evaluation. includes, i) theoretical source profiling (PSP, i.e., size capacity estimation from municipal vs. ship sewage disposal) scenario hypothesis generation, ii) high-resolution field assessment levels...
Abstract Vibrio cholerae are natural inhabitants of specific aquatic environments. Strains not belonging to serogroups O1 and O139 usually unable produce cholera toxin cause cholera. However, non‐toxigenic V. (NTVC) able a variety mild‐to‐severe human infections (via seafood consumption or recreational activities). The number unreported cases is considered substantial, as NTVC notifiable physicians mostly unaware this pathogen. In the northern hemisphere, have been reported increase due...
Running cold and hot water in buildings is a widely established commodity. However, interests regarding hygiene microbiological aspects had so far been focussed on water. Little attention has given to the microbiology of domestic hot-water installations (DHWIs), except for pathogenic Legionella. World-wide, regulations consider (or warm) as 'heated drinking water' that must comply (cold) (DW) standards. few reports exist indicate presence growth microbial flora DHWIs, even when supplied with...
A novel DNA strip test enables the detection of low amounts 35S promoter genetically modified organisms.
Acting through a combination of direct and indirect pathogen clearance mechanisms, blood-derived antimicrobial compounds (AMCs) play pivotal role in innate immunity, safeguarding the host against invading microorganisms. Besides their activity, some AMCs can neutralize endotoxins, preventing interaction with immune cells avoiding an excessive inflammatory response. In this study, we aimed to investigate influence unfractionated heparin, polyanionic drug clinically used as anticoagulant, on...
We report a novel molecular assay, based on helicase-dependent amplification (HDA), for the detection of enterococci as markers fecal pollution in water. This isothermal assay targets same Enterococcus 23S rRNA gene region existing quantitative polymerase chain reaction (qPCR) assays U.S. Environmental Protection Agency Methods 1611 and 1609 but can be entirely performed simple heating block. The developed HDA successfully discriminated 15 enterococcal from non-enterococcal reference strains...
Abstract During recent years, mid-infrared (MIR) spectroscopy has matured into a versatile and powerful sensing tool for wide variety of analytical tasks. Attenuated total reflection (ATR) techniques have gained increased interest due to their potential perform non-destructive tasks close real time. In ATR, the essential component is sampling interface, i.e., ATR waveguide its material properties interfacing sample with evanescent field ensuring efficient photon-molecule interaction. Gallium...
<title>Abstract</title> Background Spores produced by the filamentous fungus <italic>Aspergillus niger</italic> are abundant in a variety of environments. The proliferation this indoor environments has been associated to health risks and its conidia can cause allergic reaction severe invasive disease animals humans. Therefore, detection monitoring <italic>Aspergillus</italic> is utmost importance prevent serious fungal infections contaminations. Among others, aptamers could serve as...
Abstract Background Spores produced by the filamentous fungus Aspergillus niger are abundant in a variety of environments. The proliferation this indoor environments has been associated to health risks and its conidia can cause allergic reaction severe invasive disease animals humans. Therefore, detection monitoring is utmost importance prevent serious fungal infections contaminations. Among others, aptamers could serve as biosensors for specific spores. Results In study, DNA A. were...
Abstract DNA- and RNA-based diagnostics play a pivotal role in accurately detecting characterizing health-relevant bacteria, offering insights into bacterial presence, viability treatment efficacy. Herein, we present the development of novel extraction protocol for both DNA RNA, designed to enable simple rapid molecular diagnostics. The method is based on hydrophilic ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate silica-coated magnetic beads. First, developed an IL-based cell lysis...