A5030406685- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Eosinophilic Disorders and Syndromes
- Immune Cell Function and Interaction
- Immune cells in cancer
- Atherosclerosis and Cardiovascular Diseases
- Hematological disorders and diagnostics
- Single-cell and spatial transcriptomics
- Eosinophilic Esophagitis
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
Northwestern University
2021-2024
Midwestern University
2019
Femoral atherosclerotic plaques are less inflammatory than carotid histologically, but limited cell-level data exist regarding comparative immune landscapes and polarization at these sites. We investigated intraplaque leukocyte phenotypes transcriptional in 49 patients undergoing femoral (n = 23) or 26) endarterectomy using single-cell RNA-Seq (scRNA-Seq; n 13), flow cytometry 24), IHC 12). Comparative scRNA-Seq of CD45+-selected leukocytes from 9; 35,265 cells) 4; 30,655 plaque revealed...
Abstract Bone marrow is a hematopoietic site harboring multiple populations of myeloid cells in different stages differentiation. Murine bone eosinophils are traditionally identified by Siglec-F(+) staining using flow cytometry, whereas neutrophils characterized Ly6G(+) expression. However, cytometry to characterize wild-type mice, we found substantial gray areas identification these cells. mature eosinophil population constituted only minority Lin(+)CD45(+) pool (5%). A Siglec-F(−) was...
Eosinophils play surprisingly diverse roles in health and disease. Accordingly, we have now begun to appreciate the scope of functional phenotypic heterogeneity plasticity these cells. Along with tissue-recruited subsets during inflammation, there are tissue resident eosinophil phenotypes potentially longer life spans less dependency on IL-5 for survival. Current models study murine eosinophils ex vivo rely IL-5-sustained expansion from bone marrow hematopoietic progenitors. Although it does...
Abstract Glucose is a key source of energy in systemic and cellular metabolism has known pro-inflammatory properties. Metabolic disorders (diabetes, insulin resistance) associate with asthma other allergic diseases. This study sought to understand the role hyperglycemia response. First, we injected fasted Balbc/J mice intraperitoneally (i.p.) 2g/kg dextrose test whether promotes antigen sensitization. Within one hour injection, detected rapid increase blood glucose levels followed by an...
Abstract Glucose is a key source of energy in systemic and cellular metabolism has known pro-inflammatory properties. It recently been acknowledged that associations exist between metabolic disorders (diabetes, insulin resistance, obesity), asthma other allergic diseases. As the prevalence both increases, it important to understand how can influence development allergy. First, we injected fasted wild type Balbc/J mice intraperitoneally (i.p.) with 2g/kg dextrose (standard hyperglycemic...
Abstract Bone marrow, while known to be a hematopoietic site for eosinophils, also harbors resident populations of these cells. Identification such eosinophils typically requires flow cytometry-assisted sorting, with Siglec-F as key marker identification murine eosinophils. At baseline, we found that bone marrow described Siglec-F(+) constitute about 5% CD45 Although this identifies mature it may not sufficient represent all phenotypes. Using sequencing and FACS sorting approaches, the...
Abstract In allergic inflammation, eosinophils interacting with epithelial barriers experience exposure to cytokines and the extracellular milieu of airway remodeling. To better understand eosinophil activation biology in tissue, we modeled synergistic opposing regulation naïve by both sub-epithelial matrix protein tenascin C (TNC), a key player regeneration, IL-33, an damage-induced cytokine. IL-5-differentiated bone marrow-derived murine were cultured recombinant TNC (25 ug/ml), IL-33 (100...