A5039458658- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Virus-based gene therapy research
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Monoclonal and Polyclonal Antibodies Research
- Pluripotent Stem Cells Research
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- TGF-β signaling in diseases
- Chronic Myeloid Leukemia Treatments
- Multiple Myeloma Research and Treatments
- Acute Myocardial Infarction Research
- Cardiac Imaging and Diagnostics
- Diabetes and associated disorders
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Polyomavirus and related diseases
- Kruppel-like factors research
- Virology and Viral Diseases
University Hospital of Basel
2004-2020
University of Fribourg
2012
University Hospital of Geneva
2008
University Children’s Hospital Basel
2008
University Hospital Frankfurt
2008
Goethe University Frankfurt
2008
Amgen (United States)
2005
BC Cancer Agency
1998-2003
University of British Columbia
2000
Johannes Gutenberg University Mainz
2000
This study evaluated whether T-cell memory reflects increased precursor frequencies of specific long-lived T cells and/or a low-level immune response against some form persistent antigen. Antivirally protective CD8+ was analyzed mostly in the original vaccinated host to assess role antigen its maintenance. mediated resistance reinfection measured spleen and peripheral solid organs with protocols that excluded protection by antibodies. In vivo compared detectable cytotoxic T-lymphocyte...
Natural killer (NK) cells are increasingly used in clinical studies order to treat patients with various malignancies. The following review summarizes platform lectures and 2013-2015 consortium meetings on manufacturing use of NK Europe United States. A broad overview recent pre-clinical results cell therapies is provided based unstimulated, cytokine-activated, as well genetically engineered using chimeric antigen receptors (CAR). Differences donor selection, quality control for cancer...
In this report, we demonstrate that the Src homology 2 domain–containing inositol-5-phosphatase (SHIP) plays a critical role in regulating both B cell development and responsiveness to antigen stimulation. SHIP−/− mice exhibit transplantable alteration lymphoid results reduced numbers of precursor (fraction C) immature cells bone marrow. vitro, purified enhanced proliferation response receptor stimulation presence absence Fcγ IIB coligation. This enhancement is associated with increased...
We have previously shown that long-term in vitro proliferating fetal liver pre-B cell lines derived from autoimmune-prone (NZB x NZW)F1 (BW) mice, but not normal (B6 DBA2)F1 can differentiate severe combined immunodeficient (SCID) mice to produce elevated levels of serum immunoglobulin (Ig) M and IgG, high titers antinuclear antibodies The contribution parental NZB NZW strains B abnormalities BW hybrid was investigated here by preparing cells transferring them into SCID- RAG-2-targeted mice....
The effects of formalin on the infectivity and immunogenicity vesicular stomatitis virus (VSV) serotype Indiana were investigated. We found that inactivation VSV prevents infection Vero cells in a concentration- time-dependent manner, as shown by fluorometric cell analysis inhibition plaque formation. Inactivated failed to induce significant cytotoxic T-lymphocyte responses vivo or after restimulation vitro. In contrast, early immunoglobulin M (IgM) response, which is T help independent...
The size of the Ab repertoire has been estimated to comprise theoretically somewhere between > 10(10) and approximately 10(4) specificities, dependent on criteria used. In an attempt estimate anti-viral protective mouse B cell Ab-forming (AFC) frequencies neutralizing levels during course infection with vesicular stomatitis virus (VSV) were analyzed. Determination AFC frequencies, limiting dilution assays, adoptive transfer experiments SCID mice revealed that acute phase (day 8) immune...
Because of a high risk graft-versus-host disease (GVHD), donor lymphocyte infusions with unmodified lymphapheresis products are not used after haploidentical hematopoietic stem cell transplantation. Natural killer (NK) cells have antitumor activity and may consolidate engraftment without inducing GVHD. Production NK under good manufacturing practice (GMP) conditions in sufficient number is difficult.Twenty-four apheresis procedures subsequent NK-cell enrichment from 14 donors were performed....
The concept of tumor immunosurveillance has raised prospects for natural killer cell-based immunotherapy human cancer. cure acute myeloid leukemia may depend on eradication leukemic stem cells, the self-renewing component leukemia. Whether cells can recognize and lyse is not known. To develop strategies that effectively target leukemia-leukemic we investigated anti-leukemic effects alloreactive single KIR+ cells. Natural effectors with KIR specificity mismatched respect to HLA class I...
Multidrug-resistant (MDR) cytomegalovirus (CMV) emerged after transient responses to ganciclovir, foscarnet, and cidofovir in a CMV-seropositive recipient who underwent allogeneic hematopoietic stem cell transplantation from CMV-seronegative donor. Experimental treatments using leflunomide artesunate failed. Re-transplantation donor supported by adoptive transfer of pp65-specific T cells maribavir was followed lasting suppression. This case illustrates that successful MDR CMV therapy may...
To investigate the feasibility and safety of haploidentical natural killer (NK) cell infusions as consolidation immunotherapy after autologous stem transplant (ASCT) in patients with plasma myeloma.Ten (median age, 59 years) received induction treatment followed by high-dose melphalan (200 mg/m2) at day -1, ASCT 0 increasing NK doses (1.5 × 106, 1.5 107 multiple 1.0 108 cells/kg body weight) from +1 to +30 ASCT. cells were harvested purified peripheral blood donors expanded for 19 days...