A5101618958- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Bacillus and Francisella bacterial research
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Autoimmune and Inflammatory Disorders Research
- Computational Drug Discovery Methods
- Piperaceae Chemical and Biological Studies
- Plant Disease Resistance and Genetics
- Poxvirus research and outbreaks
- Animal Virus Infections Studies
- Alkaloids: synthesis and pharmacology
- Essential Oils and Antimicrobial Activity
- Insect and Pesticide Research
- Parvovirus B19 Infection Studies
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Plant-Microbe Interactions and Immunity
- Berberine and alkaloids research
- Respiratory viral infections research
Chinese Academy of Sciences
2018-2025
Wuhan Institute of Virology
2018-2025
University of Chinese Academy of Sciences
2018-2024
Shandong Agricultural University
2021-2022
Lassa virus (LASV) belongs to the Mammarenavirus genus (family Arenaviridae) and causes severe hemorrhagic fever in humans. At present, there are no Food Drug Administration (FDA)-approved drugs or vaccines specific for LASV. Here, high-throughput screening of an FDA-approved drug library was performed against LASV entry by using pseudotype bearing envelope glycoprotein (GPC). Two hit compounds, lacidipine phenothrin, were identified as inhibitors micromolar range. A mechanistic study...
The mosquito-borne Japanese encephalitis virus (JEV) causes serious illness worldwide that is associated with high morbidity and mortality. Currently, there are no effective drugs approved for the treatment of JEV infection. Drug-repurposing screening an alternative approach to discover potential antiviral agents.
Lassa virus (LASV) belongs to the Old World Mammarenavirus genus (family Arenaviridae). At present, there are no approved drugs or vaccines specific for LASV. In this study, high-throughput screening of a botanical drug library was performed against LASV entry using pseudotype bearing envelope glycoprotein complex (GPC). Two hit compounds, bergamottin and casticin, were identified as micromolar range inhibitors entry. A mechanistic study revealed that casticin inhibited by blocking low...
Objective: Lassa virus (LASV) glycoprotein complex (GPC) contains retained stable-signal peptide (SSP), 1 (GP1), and 2 (GP2). Through serial passaging of LASV with inhibitors, adaptive mutants were obtained, most which had mutations in the transmembrane (TM) domain GP2. Characterizing fusion inhibitor target within TM GP2 provided insights for development drugs vaccines. Methods: We conducted membrane fusion, IIH6 inhibition, thermostability, viral growth kinetics assays to characterize...
Abstract Monkeypox virus (MPXV), an orthopoxvirus that has long been endemic in Africa, posed a significant global health threat since 2022. The I7L protease, highly conserved cysteine proteinase essential for replication, represents promising target broad‐spectrum antiviral drug development. Here, the first crystal structure of MPXV protease is reported, revealing its unique dimeric form and different conformations cap region nearby active site. Molecular dynamics simulations AlphaFold3...
BackgroundSevere fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne pathogen that causes severe hemorrhagic in humans, but no FDA-approved specific antivirals or vaccines are available to treat prevent SFTS.MethodsThe plasmids construction and transfection were performed generate the recombinant SFTSV harboring nanoluciferase gene (SFTSV-Nluc). Immunostaining plaque assay was measure viral titers, DNA electrophoresis Sanger sequencing evaluate genetic stability....
An escalating pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has severely impacted global health. There is a lack of specific treatment options for diseases SARS-CoV-2. In this study, we used pseudotype virus (pv) containing SARS-CoV-2 S glycoprotein to screen botanical drug library 1037 drugs identify agents that prevent entry into cell. Our study identified four hits, including angeloylgomisin O, schisandrin B, procyanidin, and oleanonic acid, as...
Abstract Zika virus (ZIKV) is an infectious disease that has become important concern worldwide, it associates with neurological disorders and congenital malformations in adults, also leading to fetal intrauterine growth restriction microcephaly during pregnancy. However, there are currently no approved vaccines or specific antiviral drugs for preventing treating ZIKV infection. Here, we show two FDA-approved Na + /K -ATPase inhibitors, ouabain digoxin, can block infection at the replication...
Lujo virus (LUJV) belongs to the Old World (OW) genus Mammarenavirus (family Arenaviridae). It is categorized as a biosafety level (BSL) 4 agent. Currently, there are no U.S. Food and Drug Administration (FDA)-approved drugs or vaccines specifically for LUJV other pathogenic OW mammarenaviruses. Here, high-throughput screening of an FDA-approved drug library was conducted using pseudotype viruses bearing envelope glycoprotein (GPC) identify inhibitors entry. Three hit compounds, trametinib,...
Mosquito-borne Japanese encephalitis virus (JEV) causes serious illness worldwide and is associated with high morbidity mortality. To identify potential host therapeutic targets, a high-throughput receptor tyrosine kinase small interfering RNA library screening was performed recombinant JEV particles.
Abstract The functions of long noncoding (lnc) RNA in the response maize to infection by rice black streaked dwarf virus (RBSDV) is poorly understood, and therefore, a comprehensive disease‐responding lncRNA profile infected with RBSDV was investigated. A total 38,511 mRNAs 2717 lncRNAs were identified performing transcriptomic analysis leaves virus, including 558 8276 differentially expressed (DElncRs) (DEmRs). Functional enrichment DEmRs showed that multiple immune‐related pathways...
Background: The COVID-19 pandemic underscores the need for effective models to study SARS-CoV-2 infection and evaluate antiviral therapies. Objectives: This aimed develop a human nasal organoid model assess susceptibility its variants effects of compounds such as camostat, remdesivir, bergamottin. Methods: Nasal organoids were infected with HCoV-OC43 pseudoviruses, followed by wild-type variant strains in BSL-3 laboratory. Viral content was measured at 2, 24, 48 hours post-infection using...
<title>Abstract</title> The LASV glycoprotein complex (GPC) contains a retained stable signal peptide (SSP), GP1, and GP2. SSP interacts with GP2 provides an interface targeted by numerous fusion inhibitors. Serialpassaging of inhibitors allowed some adaptive mutants to be obtained, most which had mutations located in the transmembrane (TM) domain In current study, we focused on F446L mutant, is reported confer resistance ST-series We found that cells conferred cross-resistance structurally...
ABSTRACT An escalating pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is impacting global health. Specific treatment options for diseases SARS-CoV-2 are largely lacking. Herein, we used a pseudotype virus (pv) bearing S glycoprotein to screen botanical drug library identify an agent against entry. All four hits, including angeloylgomisin O, schisandrin B, procyanidin, and oleanonic acid, were identified effective inhibition of pv entry in micromolar...
Abstract Lassa virus (LASV) belongs to the Old World Mammarenavirus genus (family Arenaviridae ) and is classified as a category A biological threat agent. At present, there are no approved drugs or vaccines specific for LASV. In this study, high-throughput screening of botanical drug library was performed against LASV entry using pseudotype bearing envelope glycoprotein (GPC). Two hit compounds, bergamottin casticin, were identified inhibitors in micromolar range. mechanistic study revealed...
Abstract Lassa virus (LASV) belongs to the Mammarenavirus genus (family Arenaviridae) and causes severe hemorrhagic fever in humans. The glycoprotein precursor (GPC) contains eleven N-linked glycans that play essential roles GPC functionalities such as cleavage, transport, receptor recognition, epitope shielding, immune response. We used three mutagenesis strategies abolish individual glycan chains on found all mutations led cleavage inefficiency 2 nd , 5 th 8 glycosylation motifs. To...