A5101706976- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Microbial Natural Products and Biosynthesis
- Bacterial Genetics and Biotechnology
- Toxin Mechanisms and Immunotoxins
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Pneumonia and Respiratory Infections
- Telemedicine and Telehealth Implementation
- COVID-19 and Mental Health
- Stroke Rehabilitation and Recovery
- Plant-Microbe Interactions and Immunity
- Mobile Health and mHealth Applications
- Cancer Research and Treatments
- Allergic Rhinitis and Sensitization
- Genomics and Phylogenetic Studies
- Genetic Associations and Epidemiology
- Transgenic Plants and Applications
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Plant Molecular Biology Research
- Lower Extremity Biomechanics and Pathologies
- Family Caregiving in Mental Illness
- Foot and Ankle Surgery
- COVID-19 and healthcare impacts
Center for Clinical Research (United States)
2023
Stanford University
2023
Fair Haven Community Health Center
2021
University of Birmingham
2019
National Center for Immunization and Respiratory Diseases
2016-2017
Centers for Disease Control and Prevention
2016-2017
Brown University
2004
University of Leicester
1982-1993
Carnegie Mellon University
1987-1993
University of Massachusetts Amherst
1988
A methylase enzyme, responsible for autoimmunity in the thiostrepton producer Streptomyces azureus, renders ribosomes completely resistant to thiostrepton. This RNA-pentose modifies adenosine-1067 of Escherichia coli 23 S rRNA.
ObjectivesOur objective was to evaluate and exploit a whole genome sequence (WGS) bioinformatics pipeline for predicting antimicrobial resistance capsular serotypes from invasive group B streptococci (iGBS).MethodsFor 1975 iGBS recovered during 2015 CDC's Active Bacterial Core surveillance, we compared predictions with broth dilution testing. Fifty-six isolates earlier surveillance were included testing β-lactams. Conventional serotyping WGS-based assignments 302 isolates.ResultsAll 28...
Our whole genome sequence (WGS) pipeline was assessed for accurate prediction of antimicrobial phenotypes. For 2316 invasive pneumococcal isolates recovered during 2015 we compared WGS data to broth dilution testing (BDT) 18 antimicrobials. 11 antimicrobials categorical discrepancies were assigned when WGS-predicted MICs and BDT predicted different categorizations susceptibility, intermediate resistance or resistance, ranging from 0.9% (tetracycline) 2.9% (amoxicillin). β-lactam antibiotics,...
We have investigated the mechanisms whereby equimolar quantities of ribosomal proteins accumulate and assemble into ribosomes yeast Saccharomyces cerevisiae. Extra copies cry1 or RPL16 genes encoding rp59 L16 were introduced by transformation. Excess mRNA accumulated in polyribosomes these cells was translated at wild-type rates proteins. These excess degraded until their levels reached those other Identical results obtained when transcription RPL16A rapidly induced using GAL1-RPL16A...
Determinants of antibiotic resistance have been cloned from four antibiotic-producing streptomycetes into Streptomyces lividans. Biochemical analyses resistant clones revealed the presence enzymes that had previously characterized as likely determinants in producing organisms. These included: 23S rRNA methylases S. azureus and erythreus, which confer to thiostrepton erythromycin, respectively; viomycin phosphotransferase vinaceus; aminoglycoside acetyltransferase neomycin producer fradiae....
The Saccharomyces cerevisiae CRY1 gene encodes ribosomal protein rp59, a component of the 40S subunit. Mutations in can confer resistance to alkaloid cryptopleurine, an inhibitor elongation step translation. nucleotide sequence cloned was determined. predicted amino acid shows that 14,561-dalton polypeptide has 88% homology hamster or human S14 responsible for emetine and 45% Escherichia coli S11. Analysis DNA sequences upstream from revealed presence three sequences, HOMOL1 (consensus,...
The mode of action berninamycin on bacterial protein synthesis is related to that thiostrepton, a dissimilar compound. Both antibiotics bind the complex 23S RNA with L11 and both affect various functions ribosomal A site. Also, Streptomyces bernensis azureus (which, respectively, produce thiostrepton) possess similar methylases capable rendering ribosomes resistant these compounds. Resistance involves specific pentose-methylation RNA.
The antibiotics thiostrepton, siomycin, and sporangiomycin are closely related both in structure mode of action. Actinomycetes which produce this group compounds possess ribonucleic acid-pentose methylases, act upon 23S ribosomal acid render ribosomes resistant to the action these antibiotics. This is achieved via formation a single residue 2'-O-methyladenosine per ribosome.
Ribosomes from the extreme thermophile Thermus thermophilus are capable of translation in a coupled transcription–translation system derived Escherichia coli. At 45°C, T.thermophilus ribosomes translate at ∼25–30% maximal rate E.coli ribosomes, and synthesize full-length protein. subunits can be combined to effect translation, with spectrum proteins produced depending upon source 30S subunit. In this system, function concert translational factors tRNAs, elongation release being supplied...
The Saccharomyces cerevisiae CRY1 gene encodes ribosomal protein rp59, a component of the 40S subunit. Mutations in can confer resistance to alkaloid cryptopleurine, an inhibitor elongation step translation. nucleotide sequence cloned was determined. predicted amino acid shows that 14,561-dalton polypeptide has 88% homology hamster or human S14 responsible for emetine and 45% Escherichia coli S11. Analysis DNA sequences upstream from revealed presence three sequences, HOMOL1 (consensus,...
Indoor tanning beds cause more than 450,000 new skin cancers each year, yet their use remains common, with a global indoor prevalence of 10.4%. Social media provides an opportunity for cost-effective, targeted public health messaging. We sought to direct Instagram users at high risk accurate information about the risks and reduce bed use.
Abstract The Saccharomyces cerevisiae CRY1 gene encodes the 40S ribosomal subunit protein rp59 and confers sensitivity to synthesis inhibitor cryptopleurine. A yeast strain containing cry1-delta 1::URA3 null allele is viable, cryptopleurine sensitive (CryS), expresses mRNA, suggesting that there a second functional CRY gene. CRY2 has been isolated from genomic library cloned in bacteriophage lambda, using DNA probe. sequence of contains an open reading frame encoding 59 differs at five...
Journal Article Sequence and genetic analysis of a dispensible 189 nucleotide snRNA from Saccharomyces cerevisiae Get access J. Ryan Thompson, Thompson † Present address: Department Biological Sciences, Carnegie-Mellon University, Pittsburgh, PA 15213, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar John Zagorski, Zagorski L. Woolford, Woolford 1Department UniversityPittsburgh, Maurille Fournier * To whom correspondence should be addressed Nucleic Acids...
Background Early intervention services aim to improve outcomes for people with first episode psychosis and, where possible, prevent psychiatric hospital admission. When hospitalisation does occur, inpatient staff are required support patients and families who may be less familiar services, uncertain about possible outcomes, experiencing a the time. Aims Our study aimed understand process of in early psychosis, from perspective nursing staff. We were particularly interested their experiences...
Correction for ascertainment bias is a vital part of the analysis genetic epidemiology studies that needs to be undertaken whenever subjects are not recruited at random. Adjustment often requires extensive numerical integration, which can very slow or even computationally infeasible, especially if model includes many fixed and random effects. In this paper we propose two-stage method correction. first stage estimate parameters pertain ascertained population, population would selected into...
Click to increase image sizeClick decrease sizeKeywords: Footwearinjurykneerunninggait Disclosure statementNo potential conflict of interest was reported by the author(s).
Program Goals: During the COVID-19 pandemic, anticipatory guidance, developmental surveillance andimmunizations may be postponed At Fair Haven Community Health Care (FHCHC), a multi-site communityhealth center (CHC) in New Haven, CT, serving approximately 18,000 patients (9,000 children), weimplemented cohorting during period of greatest local impact pandemic Our goals were to (1)prioritize well-child care for children due immunizations, (2) increase capacity telemedicine forpatients with...