A5007722996- Lipid Membrane Structure and Behavior
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Lysosomal Storage Disorders Research
- Neuroscience and Neural Engineering
- Viral Infections and Outbreaks Research
- Neuroinflammation and Neurodegeneration Mechanisms
- EEG and Brain-Computer Interfaces
- Bacteriophages and microbial interactions
- Electrochemical Analysis and Applications
- Neural dynamics and brain function
- Conducting polymers and applications
- Nanoparticle-Based Drug Delivery
University of Cambridge
2021-2025
Super-resolution optical imaging reveals the accumulation of SARS-CoV-2 nucleocapsid protein at viral replication organelles.
Although fusogenic liposomes offer a promising approach for the delivery of antibiotic payloads across cell envelope Gram-negative bacteria, there is still limited understanding individual nanocarrier interactions with bacterial target. Using super-resolution microscopy, we characterize interaction dynamics positively charged (Escherichia coli) and Gram-positive (Bacillus subtilis) bacteria. The merge outer membrane (OM) while attachment or lipid internalization observed in cells. Employing...
Microelectrode arrays (MEAs) permit recordings with high electrode counts, thus generating complex datasets that would benefit from precise neuronal spike sorting for meaningful data extraction. Nevertheless, conventional methods face limitations in recognizing diverse shapes. Here, we introduce PseudoSorter, which uses self-supervised learning techniques, a density-based pseudolabeling strategy, and an iterative fine-tuning process to enhance accuracy. Through benchmarking, demonstrate the...
Abstract Simultaneously recording network activity and ultrastructural changes of the synapse is essential for advancing understanding basis neuronal functions. However, rapid millisecond‐scale fluctuations in subtle sub‐diffraction resolution synaptic morphology pose significant challenges to this endeavor. Here, specially designed graphene microelectrode arrays (G‐MEAs) are used, which compatible with high spatial imaging across various scales as well permit temporal electrophysiological...
Abstract Simultaneously recording network activity and ultrastructural changes of the synapse is essential for advancing our understanding basis neuronal functions. However, rapid millisecond-scale fluctuations in subtle sub-diffraction resolution synaptic morphology pose significant challenges to this endeavour. Here, we use graphene microelectrode arrays (G-MEAs) address these challenges, as they are compatible with high spatial imaging across various scales well temporal...
ABSTRACT Although fusogenic liposomes offer a promising approach for the delivery of antibiotic payloads across cell envelope Gram-negative bacteria, there is still limited understanding individual nanocarrier interactions with bacterial target. Using super-resolution microscopy, we characterize interaction dynamics positively charged ( Escherichia coli ) and Gram-positive Bacillus subtilis bacteria. The merge outer membrane (OM) while attachment or lipid internalization observed in cells....
Abstract Extracellular recordings of neuronal activity constitute a powerful tool for investigating the intricate dynamics neural networks and individual neurons. Microelectrode arrays (MEAs) allow with high electrode count, ranging from 10s to 1000s, generating extensive datasets information. Furthermore, MEAs capture extracellular field potentials cultured cells, resulting in highly complex signals that necessitate precise spike sorting meaningful data extraction. Nevertheless,...
Abstract Despite being the target of extensive research efforts due to COVID-19 pandemic, relatively little is known about dynamics SARS-CoV-2 replication within cells. We investigate and characterise tightly orchestrated sequence events during different stages infection cycle by visualising spatiotemporal four structural proteins at high resolution. The nucleoprotein expressed first accumulates around folded ER membranes in convoluted layers that connect viral RNA foci. find three...