A5091148477- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Receptor Mechanisms and Signaling
- Axon Guidance and Neuronal Signaling
- SARS-CoV-2 and COVID-19 Research
- Lipid Membrane Structure and Behavior
- Neurogenetic and Muscular Disorders Research
- Viral Infections and Outbreaks Research
- Amyotrophic Lateral Sclerosis Research
- Advanced Fluorescence Microscopy Techniques
- Biotin and Related Studies
- Cellular transport and secretion
- Retinal Development and Disorders
- ATP Synthase and ATPases Research
- Renal Diseases and Glomerulopathies
- Single-cell and spatial transcriptomics
University of Cambridge
2019-2024
Super-resolution optical imaging reveals the accumulation of SARS-CoV-2 nucleocapsid protein at viral replication organelles.
Extrinsic cues trigger the local translation of specific mRNAs in growing axons via cell surface receptors. The coupling ribosomes to receptors has been proposed as a mechanism linking signals but it is not known how broadly this operates, nor whether can selectively regulate mRNA translation. We report that receptor-ribosome employed by multiple guidance cue and interaction mRNA-dependent. find different associate with distinct sets RNA-binding proteins. Cue stimulation
Abstract Immunofluorescence microscopy is routinely used in the diagnosis of and research on renal impairments. However, this highly specific technique restricted its maximum resolution to about 250 nm lateral 700 axial directions thus not sufficient investigate fine subcellular structure kidney’s glomerular filtration barrier. In contrast, electron offers high resolution, but comes at cost poor preservation immunogenic epitopes antibody penetration alongside a low throughput. Many these...
Aberrant condensation and localization of the RNA-binding protein (RBP) fused in sarcoma (FUS) occur variants amyotrophic lateral sclerosis (ALS) frontotemporal dementia (FTD). Changes RBP function are commonly associated with changes axonal cytoskeletal organization branching neurodevelopmental disorders. Here, we asked whether defects also vivo a model FUS-associated disease. We use two reported Xenopus models ALS/FTD (of either sex), ALS-associated mutant FUS(P525L) mimic hypomethylated...
Abstract During neuronal wiring, extrinsic cues trigger the local translation of specific mRNAs in axons via cell surface receptors. The coupling ribosomes to receptors has been proposed as a mechanism linking signals but it is not known how broadly this operates, nor whether can selectively regulate mRNA translation. We report that receptor-ribosome employed by multiple guidance cue and interaction mRNA-dependent. find different bind distinct sets RNA-binding proteins. Cue stimulation...
Abstract Despite being the target of extensive research efforts due to COVID-19 pandemic, relatively little is known about dynamics SARS-CoV-2 replication within cells. We investigate and characterise tightly orchestrated sequence events during different stages infection cycle by visualising spatiotemporal four structural proteins at high resolution. The nucleoprotein expressed first accumulates around folded ER membranes in convoluted layers that connect viral RNA foci. find three...
Summary Aberrant condensation and localisation of the RNA-binding protein fused in sarcoma (FUS) occur variants amyotrophic lateral sclerosis (ALS) frontotemporal dementia (FTD). ALS is also associated with cytoskeletal defects, genetically through observations compromised axonal transport. Here, we asked whether organisation an early feature FUS-associated ALS/FTD. We used ALS-associated mutant FUS(P525L) FTD-mimic hypomethylated FUS, FUS(16R), to investigate common distinct changes found...
Extrinsic cues trigger the local translation of specific mRNAs in growing axons via cell surface receptors. The coupling ribosomes to receptors has been proposed as a mechanism linking signals but it is not known how broadly this operates, nor whether can selectively regulate mRNA translation. We report that receptor-ribosome employed by multiple guidance cue and interaction mRNA-dependent. find different associate with distinct sets RNA-binding proteins. Cue stimulation Xenopus retinal...